We narrowed to 632 results for: ncl

...decrease these so-called “off-target effects” have included using a Cas9 nickase variant, lowering Cas9 expression...

... Someone could take on an engineering project including hundreds of genes and regulation and resource ...

...This has been used for a wide range of purposes, including activating an oncogene only in a particular organ...

...these solutions below. Alternatively, companies including ThermoFisher, Millipore Sigma, and Azure Biosystems...

...example of an individual part, this category also includes promoters, UTRs, terminators, etc. Once selected...

...selected by the ‘quality’ of the gRNA sequence, including propensity for off-targets, secondary structures...

...events-of-interest. Sensors suitable for ER measurements include: CEPIA (additional versions are available to simultaneously...

...three or four base-pair sequences, and exclude or include specific overhang sequences, allowing it to be ...

...al., 2016).   DREADD ligands Several DREADDs, including hM3D(Gq), have been derived from muscarinic receptors...

...replicating and spreading in non-target cells, including yourself as the researcher.   Figure 1:...

... Find plasmids for other developmental models including flies, nematodes, and zebrafish Find plasmids ...

...Split-BioID’s key advantage over other PPI methods, including BioID, is its spatiotemporal controlled labeling...

...your lab can do to get into the robotics spirit include: Start using electronic multichannel and single...

...promoters across these three species, the Roska lab concluded that a promoter’s ability to target a cell class...

...Twitter list contains >300 Twitter literature bots, including a bot for biosensor papers, microbe articles, ...

...creating plasmids encoding affinity reagents. These include conventional heavy and light chain recombinant ...

...defined manner. This allows scientists to draw conclusions due to gene expression in a specific brain area...

...Addgene’s CRISPR-based Protein Tagging collection includes systems for tagging mammalian cell lines, Drosophila...

...unfortunate experience of witnessing…nothing. For some unclear reason, cells are not getting transduced. Here,...

...pair of loxP sites and a pair of FRT sites and include both Cre and FLP recombinases.   Figure ...