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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 792 results for: por

Showing: 541 - 560 of 792 results

  1. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ... provides a useful yet low-pressure teaching opportunity for lab members or students new to protein expression...induce high levels of gene activation for both reporter and endogenous genes. It is highly specific in...

  2. Clear and Accessible Writing

    Type
    Blog Post
    Published
    Feb. 15, 2024, 2:15 p.m.
    ...reader would glean from the image itself.  As we incorporated alt text into our work, we began to realize ..., and we’d love to hear yours as well.  More importantly, you can use these four steps for any type of...

  3. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...the target cell or transiently expressed. It’s important to note that for all the applications we discuss... plasmids can induce gene-specific silencing temporarily, long-term, and even inducibly depending on the...

  4. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ... cell membrane. Heterotrimeric G proteins are important molecular switches in signal transduction pathways...which can then activate expression of a luciferase reporter in the nucleus. The Roth lab showed that this ...

  5. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    Published
    Dec. 11, 2018, 12:46 p.m.
    ...NPR, articles in magazines like Fast Company, opportunities to speak at conferences, and offers for postdoc...can fit into a researcher’s work day. It’s also important to remember that it’s not the amount of time you...

  6. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ...from the mol bio side you’re sure to have many opportunities to learn about and develop technologies that...FRET-based biosensing technique can be applied to an important neuronal signalling pathway - if you’ve got a ...

  7. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...sequence flexibility indicates that gRNAs could be incorporated into these constructs without compromising cleavage...slightly higher mutation rates than they’d previously reported in protoplasts (15% and 9% of target sites.) How...

  8. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...process involve repairing chromosomal DSBs. It is important to know that cells possess two major repair pathways...chance of causing a frameshift mutation. Of some importance, the deletion can be less heterogeneous when ...

  9. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...): These are considered low copy vectors and incorporate part of an ARS along with part of a centromere...nutrient, the host cells will die unless they have incorporated the plasmid carrying the required gene.  The...

  10. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ...plasmids have been introduced by transfection or electroporation. All you do to create a protein that binds ...where particular variants or mutations have been reported in people, we can use this kit to knock in those...

  11. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ...biology known as bioelectricity is revealing the important role of bioelectrical signaling mediated by ion-channels...technologies are now available for fluorescent reporting and genetically encoded fluorescent voltage indicators...

  13. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...interacting proteins, which results in poor spatiotemporal resolution for in vivo work. APEX2, a faster...identify highly biotinylated background proteins reported by other research groups that performed BirA*-...

  14. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...combinatorial libraries, and high specificity has been reported for a number of targets. Due to their size and...ain't broke, don't fix it!  That being said, it's important to know all your options when planning and running...

  15. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...tended to be very large and often required the incorporation of a cleavage site and a post-purification cleavage...very pure sample. Because binding strength is proportional to the length of the tag, with longer tags you...

  16. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...for IgG subtypes from different animals so it’s important to choose the reagent that works for your species...sure the staining you are seeing is real. It’s important to include a negative control using serum from...

  19. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ...Thesis (who has also blogged for us) offers a free report on her website called “Secrets to Success in Graduate...be helpful having a coach that can offer extra support for issues of diversity in science.  A great person...
Showing: 541 - 560 of 792 results