Overcoming the AAV Size Limitation for CRISPR Delivery
Type
Blog Post
Published
Sept. 16, 2020, 1 p.m.
...immunogenicity and range of serotypes allowing preferential infection of certain tissues. However, packaging...equally potent Cas9 protein, the Zhang lab took a different approach. They analyzed over 600 Cas9 orthologs...Sontheimer lab used NmeCas9 with AAV to edit two different disease-causing loci in mouse liver.
Two other...have also been used to express base editors.
References
Chew WL, Tabebordbar M, Cheng JKW, Mali P, Wu...