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  1. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...Interview with Nicola Patron on MoClo Plasmids 101: Golden Gate Cloning ... Susanna Stroik...

  2. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...want to knock out a gene, a frameshift mutation introduced anywhere within the early coding region will ...  Susanna Stroik...

  4. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ...into the robotics spirit include: Start using electronic multichannel and single channel pipettes. These...by one. It seems very simplistic and it isn’t electronic but it is one of the most helpful machines I ...

  5. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...sites on the target protein they tend to have a strong signal and to work in a variety of applications...Postembedding Immunocytochemical Techniques for Light and Electron Microscopy. In Methods in Cell Biology (pp. 283...

  7. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    Published
    June 25, 2020, 1:15 p.m.
    ...Sequencing Analyst intern. As a member of the Quality Control team, I help make sure the plasmids that go out...was really interested to learn more when I was introduced to bioinformatics in the previous programs. I...

  10. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ... easiest to adapt to genome editing, but the introduction of type V Cpf1-driven systems added another ... endjoining (NHEJ). Cas9-mediated NHEJ usually destroys the PAM site due its proximity to the cleavage...

  11. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    Published
    Jan. 21, 2015, 1:30 p.m.
    ...who spend most of their time moaning about their troubles, Silent or unresponsive clams, who seem to ignore...Indecisives, who keep changing their minds and have trouble making decisions. In my work as a thesis coach...

  12. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...ll need to produce your viruses in-house.   Introducing the systems There are many ways that viruses ...insectspodoptera frugiperda (lepidoptera; noctuidae). In Vitro, 13(4), 213–217. https://doi.org/10.1007/bf02615077...

  14. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...apart is the way they generate their color: they strongly absorb visible light to give colors in ambient...genome, it is not expressed as it is under the control of a rhamnose-inducible promoter. The resolution...

  15. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...not that I would know about this… A no template control (NTC) should always be included. While the physical...recombinant adeno-associated viral vectors for in vitro and in vivo use. Current protocols in molecular ...

  16. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...protein’s structure into experimentally determined electron density, have been known to say something silly...educators. Using these hands-on tools, educators introduce their students to this otherwise invisible molecular...

  17. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...used to tag endogenous genes and simultaneously introduce a selection marker for screening modified strains...with a Cas9 expression vector. They can also be introduced directly into a Drosophila S2 cell line stably...

  19. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...image acquisition is performed using cameras with electron multiplying couple-charged device (EMCCD) or complementary...use of viruses allows much better spatiotemporal control over labeling methods as compared to transgenic...
Showing: 541 - 560 of 739 results