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We narrowed to 759 results for: tro

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Showing: 541 - 560 of 759 results

  1. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ...other than to sustain those techniques. We feel strongly that the scientific and the agricultural community...aesthetics with this technology and giving consumers control over their interaction with it, we hope to inspire...

  2. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ..., Wang, Hou, Zhonggang, Xiao, Yibei, et. al. Introducing a Spectrum of Long-Range Genomic Deletions in...  Susanna Stroik...

  3. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...of the two fragments. However, rigidity can be introduced through mutation, allowing diabodies to be used...FluorophoresAntibodies 101: scFVs Antibodies 101: Introduction to Antibodies Additional resources on addgene.org...

  4. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...for cellular delivery of RNAs By Mike Lacy The retroviral-like protein PEG10 specifically binds its own...small insertions to allow in-frame deletions and introduce epitope tags and RNA transcription start sites...

  5. Click Biology: How to Click and Play to Explore Biology

    Type
    Blog Post
    Published
    Oct. 21, 2025, 1:15 p.m.
    ...triggering new cellular behavior like degradation or electrophile signaling. Many of these tools can be found ...References Devaraj, N. K., & Finn, M. G. (2021). Introduction: Click Chemistry. Chemical Reviews, 121(12),...

  6. Antibodies 101: Fab Fragments

    Type
    Blog Post
    Published
    Sept. 5, 2024, 1:15 p.m.
    ... means that F(ab’)2 fragments may have overall stronger interaction with their antigens than a Fab or ....2022.100301  Additional resources on the Addgene blog: Introduction to AntibodiesSingle Chain Fragment Variables...

  7. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...degraded. To effectively isolate intact RNA, a fast, strong protein denaturant would be required- something...late 1970’s, Chirgwin and colleagues showed that a strong protein denaturant, guanidinium thiocyanate, did...

  9. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...successfully labeled protein-coding genes with both intronic and exonic gRNAs. In fact, the method is specific...of the Pitfalls When Using Fluorescent Proteins Control Protein Activity with GFP Resources on Asddgene.org...

  10. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...the lab (for a refresher see Antibodies 101: Introduction to Antibodies.) Unfortunately, for some proteins...Protein Tags Antibodies 101: ChIP Antibodies 101: Introduction to Antibodies References Hochuli E, Bannwarth...

  11. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ...the hands of our finance team, now 3.5 Addgenies strong. The Addgene Board of Directors is mature and...and I believe they know and trust this. We’ve introduced cutting edge practices and benefits and these...

  12. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...phenotype in a scalable way. This blog post will introduce you to this new technology, and guide you through... Luke A., et al. "Genome-scale CRISPR-mediated control of gene repression and activation." Cell 159.3 ...

  13. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    Published
    Oct. 20, 2015, 2:30 p.m.
    ...Department at University of Maryland College Park, is a strong supporter of increasing the number of postdoctoral...postdoc-related data collection and analysis. He is also a strong supporter of science Master’s programs. This will...

  14. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ...their goals.  Check out Joanne's Reddit AMA I am a strong advocate for peer mentoring, but volunteer mentors...contact is Twitter’s @DrQualls. Marquita Qualls of Entropia Consulting works remotely, will travel for speaking...

  15. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    Published
    Feb. 11, 2014, 12:13 p.m.
    ...institution. Moreover, avoidance of ‘to be’ verbs creates strong, well-directed meaning and delivers strength to...will convince the reviewers that the proposal is strong, well-thought out, and will be successful when ...

  17. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ...into the robotics spirit include: Start using electronic multichannel and single channel pipettes. These...by one. It seems very simplistic and it isn’t electronic but it is one of the most helpful machines I ...

  19. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...Interview with Nicola Patron on MoClo Plasmids 101: Golden Gate Cloning ... Susanna Stroik...

  20. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...want to knock out a gene, a frameshift mutation introduced anywhere within the early coding region will ...  Susanna Stroik...
Showing: 541 - 560 of 759 results