Plasmids 101: Mammalian Vectors
Type
Blog Post
Published
March 25, 2014, 3:15 p.m.
... 293HEK
Inhibits termination step of translation
2-10 ug/mL
G418/Geneticin
neo
HeLa, NIH3T3, CHO, 293HEK...called transfection. Transfection is somewhat comparable to bacterial transformation (the introduction... a problem depending on the duration or other parameters of your experiment. Mammalian cells double at...reduces the rate of plasmid dilution but does not guarantee 100% transfection efficiency.
Stable transfection...