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Showing: 281 - 290 of 384 results
  1. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ... 293HEK Inhibits termination step of translation 2-10 ug/mL G418/Geneticin neo HeLa, NIH3T3, CHO, 293HEK...called transfection. Transfection is somewhat comparable to bacterial transformation (the introduction... a problem depending on the duration or other parameters of your experiment. Mammalian cells double at...reduces the rate of plasmid dilution but does not guarantee 100% transfection efficiency. Stable transfection...
  2. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ...create a recombinant antibody.     Figure 2: Changes to the heavy chain or light chain genes within...the antigen-binding sites of the antibody - the paratopes - may change and impact the specificity and avidity..., Aldred SF, Bestagno M, Burrone O, Maynard J, Ferrara F, Trimmer JS, Görnemann J, Glanville J, Wolf P...Versus Polyclonal Antibodies: Distinguishing Characteristics, Applications, and Information Resources. ILAR...
  3. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ..., N. and Hamilton, D. 1981. PubMed PMID: 6276558. 2. Site-specific DNA recombination in mammalian cells... it, allowing for two separate recombination events to be controlled in parallel. Have you used the Cre-lox...consisting of two 13-bp long palindromic repeats separated by an 8-bp long asymmetric core spacer sequence... which can be located either on the same or on separate pieces of DNA. Both 13bp repeat sequences on a...forming a dimer. The two loxP sites then align in a parallel orientation, allowing the four Cre proteins to...maintained).  Translocation: If the sites are on separate DNA molecules, a translocation event is generated...Cre into cells of interest. What if I need two separate recombination events? One potential limitation...
  4. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    Published
    Feb. 14, 2019, 2:35 p.m.
    ...the world’s population, we account for an estimated 2% of global plastic generation. Less than 9% of plastics... References Geyer, Roland, Jenna R. Jambeck, and Kara Lavender Law. "Production, use, and fate of all ...
  5. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    Published
    May 18, 2023, 1:15 p.m.
    ...affinity and specificity. Trends in Immunology, 29(2), 91–97. https://doi.org/10.1016/j.it.2007.11.004 ...kill the target cell. If the pathogen is a large parasite, ADCC can be induced through a very similar interaction...
  6. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    Published
    Nov. 22, 2016, 3:30 p.m.
    ...easily understood and consistent in design and layout. 2. Be concise in your writing  When writing a manuscript...worthless. But simply writing down your data doesn’t guarantee a publication. There is an art to composing manuscripts...
  7. 10 Ways to Share Your Science!

    Type
    Blog Post
    Published
    May 13, 2014, 8:34 p.m.
    ...but also something about how science really works. 2)   Read science writing in magazines or newspapers...people like a good story and science involves characters – in this case you – on a quest to ask and answer...
Showing: 281 - 290 of 384 results