...endogenous levels (Laflamme et al., 2019). CRISPR-Cas9 approaches are commonly used to produce KO cell ...is not amenable to knockout approaches. In these cases, we would recommend a knockdown approach instead...lines based on expression of the target. In this case, you could compare the antibody’s labeling of the...Biddle, M. S., Smallwood, D. T., Weston, C. A., Castells, E., Bowman, V. W., McCarthy, J., Amrani, Y., ...

... lentiviral transfer vector used to deliver both Cas9 and your gRNA of interest to mammalian cells. It... blog post on Genome-wide Screening Using CRISPR/Cas9. If you need help cloning a gRNA into lentiCRISPR...References 1. Shalem, Ophir, et al. "Genome-scale CRISPR-Cas9 knockout screening in human cells." Science 343.6166...

... frequently used elements in plant expression cassettes: plant promoters, N-terminal tags, coding sequences...C-terminal tags, plant terminators and resistance cassettes for selection of transgenic plants. The complete... gives the option to stack several expression cassettes onto a single T-DNA in subsequent rounds, dramatically...

...have one associated with the gRNA), application, cas9 species, PubMed ID, and depositor. The CRISPR applications...nick, purify, tag, scaffold, CRISPR-display, and dCas9-FokI. This table is both sortable and searchable...alphabetically or in ascending or descending order, in the case of numeric columns.  The data in any column are ...

...simultaneous gene activation in plants.  FnCas12a, unlike other Cas12a nucleases identified, functions at temperatures...editing in moderate thermophiles.  These evolved SpCas9 variants have improved activity on NAG PAMS and...

...Camelid family is composed of camels, llamas, and alpacas (and their undomesticated counterparts) - not exactly...soon after found similar antibodies in llamas and alpacas (Figure 1). At the time, researchers were already...started with immunizing a camelid (usually llamas or alpacas). But because sdAbs are so amenable to recombinant...

...at eScience Labs also write test question banks, case studies, and experiments for labs, in addition to...comprehensive exam proposals, a few PhD theses, and occasionally posting something science-related on Facebook...science education tools? Check out this Addgene podcast with educator and science rap mastermind Tom McFadden...

...skill acquisition. Classes covered filmmaking, podcasting, philosophy of science, creative writing, designing...an animated video depicting nuclear fission; a podcast with a genetics researcher on parasitoid wasps;...the face of disparate spheres of research. In my case, an interest in neuroscience was carried over and...

...unmeasurable skills and productivity coming from that casual discussion on the way to the office or in the kitchen... flexible work schedule the more we realize how casual chatter is very much missed in the virtual world...widely used and could bring back a taste of that casual kitchen chatter, ultimately sparking new ideas....

...Th2) cells from mice that constitutively express Cas9 and screen for genes that affect Th2 differentiation...Wang Lab created a tripartite system consisting of dCas9, a gRNA fused to an RNA binding motif, and a RNA-binding...protein-bEFP fusion. Thus, the bEFPs bind the UAS via the dCas9-gRNA complex which allows transcription from the...