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Showing: 281 - 290 of 422 results

  1. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ...ensuring the cells used are meant for repetitive sequences (such as STBL4 or Endura Duo), growing your bacteria... pCMV-Rep 78/68 SSM Library Amplification and Sequencing Protocol (DOCX, 32 KB) Screening Rinehart...

  2. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...itself and the degron off at the protease cleavage sequence. The tag is then shuttled to the proteasome due...the protein around, leaving a minimal additional sequence (7 kDa). This system also requires less genetic...

  4. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...circuits. Epitope tags are short antigenic peptide sequences, attached to a protein of interest (POI), that...of proteins that are highly similar in terms of sequence and thus very difficult to distinguish using standard...

  5. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...cytometric antibodies, downstream of the TUJ1 coding sequence. ES cells that stably express the dCas9-Suntag...cells were then isolated and their genomic DNA sequenced. Guide RNAs targeting several known and some unknown...

  6. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ... and G Drive. ‘Big’ data like image banks and sequencing files is likely stored in institutional and lab...to the associated data (spreadsheets, images, sequencing data, etc.) used and/or referenced in the experiment...

  8. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...Droplets that fluoresce contain the amplified target sequence. After all of the droplets have been read, the...reason, it can be used in the detection of rare sequences and single cell analysis. It is also being used...

  9. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...positive or negative selection). Next-generation sequencing (NGS) to identify genes of interest. ...Kampmann, M. (2023). CRISPR screening by AAV episome-sequencing (CrAAVe-seq) is a highly scalable cell type-specificin...

  10. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...examined approximately 1,900 sgRNAs and identified sequence features and adjustments that improved sgRNA activity...Tanenbaum et al., 2014). The sgRNA or tracrRNA sequences can also be modified to include RNA domains that...
Showing: 281 - 290 of 422 results