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Showing: 561 - 580 of 779 results

  1. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...unpublished results at a conference? Post your working manuscript as a preprint too and you can share ...American Society for Cell Biology in a position working with preprints in life sciences. He also posted...

  4. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ... ability to study and understand the complex interworking of the 86 billion neurons in the human brain... opens the door to in vivo experiments such as tracking the synaptic maturation pattern of hippocampal...

  7. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ... to own the process entirely remains a large undertaking. Priority number one was to ensure that this ... load it on to our new MiSeq and set up the run making sure to note the appropriate barcode for each sample...

  11. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...detection We and other members of the lab had been working on advancing the paper-based platform toward a ...when we needed additional expertise. Perhaps most striking is that there were seven institutions involved...

  12. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ...has slightly different activation/inactivation kinetics. For example, the light-gated cation channel, ...protein-coupled vertebrate rhodopsin in cerebellar Purkinje cells. The Journal of biological chemistry. 2011...

  14. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...setup. This requires a less involved setup when working with mice and slightly less red tape for approval...has psuedotypes. Psuedotyping is the process of making a hybrid AAV, with the genome from one serotype...

  15. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ... fluorescent proteins.  Currently, the team is working to identify how MagLOV’s structure changes under...starting point for much more. If you’re interested in working on these questions or collaborating, the York Lab...

  16. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ...bacteria can grow and compete in the same container, making it easier for a smaller, recombined plasmid to ...developing your amplification protocol! We recommend looking at protocols used for libraries that are similar...

  17. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...binding distinct PAM sequences. Now, Hu et al., working in David Liu’s lab, have gone one step further,...Once you’ve tried it, let us know how xCas9 is working for you in the comments section! References Hu...

  18. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    Published
    Aug. 4, 2016, 2:30 p.m.
    ... side, successful indel generation and epitope knockin was achieved by a few respondents suggesting that...inconvenient. Another possible reason for the trickiness of NgAgo is the inability of mature NgAgo protein...

  19. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    Published
    Sept. 24, 2015, 2:30 p.m.
    ...more CRISPR tools are being published each month, making CRISPR a great choice for your next experiment!...ties directly back to your specific goal. When thinking about step 3, however, you may be surprised at...

  20. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...don’t require a PAM sequence at the target locus, making them more flexible than Cas9/Cpf1. Some Cas13 enzymes...prefer targets with a given single base protospacer flanking site (PFS) sequence, but orthologs like LwaCas13a...
Showing: 561 - 580 of 779 results