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  1. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ...offerings. The viral vector team is publishing research, hosting conferences, and are expanding our catalog...on the Addgene website Find plasmids for your research  Learn about our viral service Check out job openings...

  2. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...make room for a gene of interest, AAVs used in research have their genomes “gutted”, meaning that all ...trunk” space for genetic cargo. Typically, when researchers speak about AAVs, they are actually referring...

  3. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...to sense their formation, and how clinicians, researchers, and even farmers are harnessing these molecules...the introduction of dsRNA. Synthetic dsRNA Researchers have been harnessing the power of dsRNA for a...

  4. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...and bind proteins. They’re used in a number of research and clinical applications. Most of the time, when...You never know what problems will arise in your research or what data you may need to collect. Understanding...

  5. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ... carefully track samples through our lab using barcoded tubes and plates. The critical pieces of information... to Verify Your Plasmid Learn How Addgene Uses Barcodes to Track Samples Resources on the Addgene Website...

  6. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...the accumulation of errors in DNA sequences. In research, these errors can affect experimental processes...importance of QC Plasmids are a fundamental part of research. They are easy to produce, easy to edit, and easy...

  7. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...! First, a quick refresher: prime editing is a “search and replace” genome editing technique that relies...Newby, G. A., Raguram, A., & Liu, D. R. (2019). Search-and-replace genome editing without double-strand...

  8. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ...gleaned from the literature and engagement with researchers who study the protein or structure of interest...introducing tags into your gene of interest. Researchers have developed a variety of endogenous gene tagging...

  9. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...This post was originally written by Tyler Ford in March 2017 and updated by Angela Holmes and Christina ...Addgene Blog Find Articles Citing Addgene Plasmids Searchable and Sortable gRNAs for Your Next CRISPR Experiment...

  10. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ...selection and expression in your target cell line, researchers often find themselves faced with a roadblock....cell line that is used for titering. Typically researchers will choose a relatively permissive cell line...

  13. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ...ever do! However, there is quite a bit of upfront research you have to do to find said robot and to find ...Biology Tips from the Repository Trenches: Using Barcodes to Track Samples Resources at Addgene.org Find...

  14. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...refractory to gene delivery methods, but the researchers was able to edit hiPSCs, mouse bone marrow (BM...plasmids. These results are in line with previous research showing that the method of Cas9 and gRNA delivery...

  15. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...fluorescent proteins derived from StayGold. ResearchSquare (Preprint). https://doi.org/10.21203/rs.3.rs... brilliance By Hridaya Patel (Addgene co-op) Researchers in the Gradinaru lab have developed an engineered...

  16. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...excitatory synapses or that are Cre-inducible, researchers can explore synaptic integration and connectivity...fluorescence. Image from Eastman et al., 2020. The researchers tested several scenarios from embryo development...

  17. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...sequences for Illumina sequencing as well as unique barcodes per sample so we can assign sequencing reads to...up the run making sure to note the appropriate barcode for each sample. About 24 hours later we unload...

  18. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...mutant allele. When all was said and done, the researchers had cured 24 mice of their disease. Meanwhile...traditional gene therapy pipelines (viral), and researchers are currently working on giving this treatment...

  19. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ...targeting. By employing various AAV serotypes, researchers can precisely direct the calcium sensor to specific...complex brain circuit dynamics in vivo. Neuroscience Research. 2021 Aug 1;169:2-8. Suzuki J, Kanemaru K, Iino...

  20. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...switches were designed as a genetic tool for researchers to conditionally manipulate gene expression in...allowing for expression of GeneR. This allows a researcher to test whether or not expressing GeneR alone...
Showing: 561 - 580 of 795 results