We narrowed to 725 results for: Cel

...small Cas protein, is active in E. coli and human cells Two years ago, Jennifer Doudna’s lab discovered ...demonstrating that CasX is active in both E. coli and human cells. Using the structure of CasX at eight different ...

... repository of CRISPR screens Cellosaurus: A knowledge resource for cell lines IBEX Knowledge Base: A ...

...outdoors in nature or visiting museums can be excellent methods to detach from the constant stream of ...to pinpoint areas of my career and life where I excel (and thus feel grateful) and identify areas in need...

... remember what happened when drug X was added to cell line Y, or the phenotype of a mouse with a specific...comparison of the same experiment from different cell lines/model systems ReFigure allows you to make...

...reading Toxic Academic Mentors by @drmellivora, an excellent blog post about workplace bullying featured on...the first place. The good news There are many excellent mentors out there. With some good pre-work you...

...these issues by creating immortal antibody-producing cell lines (hybridomas) that secrete antibodies in culture...reproducible production. However, even clonal hybridoma cell lines can produce more than one mAb (Bradbury et...

...about synthetic promoter AAVs for cell-type expression in retinal cells Resources on Addgene.org Find ...

... life that remained unexplored. Using new single-cell sequencing approaches, the lab was able to eliminate...sample was separated into smaller samples of 5-10 cells each for sequencing, allowing for lower complexity...

...these efforts is the Golden Stopwatch Award to celebrate exemplary TTOs. Our new Golden Stopwatch Award...The Golden Stopwatch Award highlights TTOs that accelerate science by processing agreements quickly! If ...

... can be used to visualize cells and to quantify targeting of particular cell types. For example, the mDlx...

.... This tag is not suitable for use in apoptotic cells as Caspase3/7 both cleave after the DVPD sequence...fluorescent marker. The GFP tag is ideal for live cell imaging, FRET, flow cytometry, or immunofluorescence...

...successfully used this plasmid to biotinylate the extracellular domain of the erythropoietin receptor and subsequently... Eye Institute and is particularly interested in cell signaling.     Additional Resources on the Addgene...

...would rather fund excellent people with mediocre ideas than mediocre people with excellent ideas. I am lucky...

...  There are many online guides – including an excellent series of posts by Joachim Goedhart on the Addgene...Associate at Harvard Medical School. He manages the Cell Biology Microscopy Facility, specializing in advanced...

...engineering project at BosLab. Once he optimizes plant cell culture and transgenesis he wants to share his knowledge... in an Open Source Lab: from CRISPR to Cats Stem Cell Models for Disease & Open Science: Interview with...

...EvolvR could also be a tool for lineage tracing cells that do not tolerate double-strand DNA breaks by...researchers to trace the progeny of a single starting cell. Additionally, multiplexing of guides allows EvolvR...

...allow scientists to express a gene of interest in a cell or animal model. AAV plasmids contain several important...Integrity on rAAV8 Production Using the Baculovirus/Sf9 Cells System. Hum Gene Ther Methods, 28(5), 277–289. https...

...as an opportunity to practice communicating.  Excellent scientists learn from advisers and other mentors...help graduate students in all STEM fields become excellent science communicators. To empower graduate students...

...team potential. I often thought that performing a cell culture experiment myself would take less time than...Another team member was highly self-motivated and excelled when given the freedom to explore projects autonomously...

...of Professor Richard Murray. Murray’s lab uses cellular extracts to perform transcription and translation...develop and test hypotheses. Students then use cellular extracts as a means to rapidly prototype DNA circuits...