We narrowed to 659 results for: Clu

1. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

   Type

   Blog Post

   Published

   Dec. 16, 2015, 3:30 p.m.

   ...decrease these so-called “off-target effects” have included using a Cas9 nickase variant, lowering Cas9 expression...

2. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

   Type

   Blog Post

   Published

   April 22, 2015, 2:06 p.m.

   ... Someone could take on an engineering project including hundreds of genes and regulation and resource ...

3. Plasmids 101: Cre-lox

   Type

   Blog Post

   Published

   Jan. 13, 2015, 3:47 p.m.

   ...This has been used for a wide range of purposes, including activating an oncogene only in a particular organ...

4. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

   Type

   Blog Post

   Published

   May 1, 2018, 1:57 p.m.

   ...Split-BioID’s key advantage over other PPI methods, including BioID, is its spatiotemporal controlled labeling...

5. Lab Automation at Addgene

   Type

   Blog Post

   Published

   March 22, 2018, 1:28 p.m.

   ...your lab can do to get into the robotics spirit include: Start using electronic multichannel and single...

6. Adenoviral Vector Production and Troubleshooting

   Type

   Blog Post

   Published

   June 14, 2018, 11:44 a.m.

   ...Successfully infected cells will become round and clump together ~3 days post infection. The amplified virus...

7. Educational Planarian Tools for Teaching Developmental Biology Techniques

   Type

   Blog Post

   Published

   May 24, 2018, 1:20 p.m.

   ... Find plasmids for other developmental models including flies, nematodes, and zebrafish Find plasmids ...

8. Plasmids for Endogenous Gene Tagging in Human Cells

   Type

   Blog Post

   Published

   April 6, 2017, 1:02 p.m.

   ...Addgene’s CRISPR-based Protein Tagging collection includes systems for tagging mammalian cell lines, Drosophila...

9. Science communication: 9 Strategies to Get Your Foot in the Door

   Type

   Blog Post

   Published

   Sept. 27, 2017, 1:23 p.m.

   ...non-profits like Phipps Conservatory or the Brilliant Club and share your knowledge at local schools. Or work...

10. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type

    Blog Post

    Published

    Aug. 20, 2019, 12:31 p.m.

    ...defined manner. This allows scientists to draw conclusions due to gene expression in a specific brain area...

11. Hot Plasmids - October 2020

    Type

    Blog Post

    Published

    Oct. 13, 2020, 1:15 p.m.

    ...page.  CasPhi is 70 kDa Cas protein identified exclusively in huge bacteriophage genomes. It’s active in...

12. Antibodies 101: Stripping and Reprobing Western Blots

    Type

    Blog Post

    Published

    Aug. 1, 2024, 1:15 p.m.

    ...these solutions below. Alternatively, companies including ThermoFisher, Millipore Sigma, and Azure Biosystems...

13. Plasmids 101: Modular Cloning

    Type

    Blog Post

    Published

    March 12, 2024, 1:15 p.m.

    ...example of an individual part, this category also includes promoters, UTRs, terminators, etc. Once selected...

14. Progress Towards a PAM-Free CRISPR

    Type

    Blog Post

    Published

    March 7, 2024, 2:39 p.m.

    ...selected by the ‘quality’ of the gRNA sequence, including propensity for off-targets, secondary structures...

15. Viral Vectors 101: Calcium Sensors

    Type

    Blog Post

    Published

    Aug. 10, 2023, 1 p.m.

    ...events-of-interest. Sensors suitable for ER measurements include: CEPIA (additional versions are available to simultaneously...

16. Viral Vectors 101: Virus Safety

    Type

    Blog Post

    Published

    June 22, 2023, 1:15 p.m.

    ...replicating and spreading in non-target cells, including yourself as the researcher.   Figure 1:...

17. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type

    Blog Post

    Published

    May 4, 2021, 1:15 p.m.

    ...creating plasmids encoding affinity reagents. These include conventional heavy and light chain recombinant ...

18. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type

    Blog Post

    Published

    July 23, 2020, 1:20 p.m.

    ...from pathogenic bacteria’s CRISPR-Cas system. Conclusion CRISPR is a great way to make those genome edits...

19. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type

    Blog Post

    Published

    June 25, 2020, 1:15 p.m.

    ...leave academia, and once she joined an investment club and discovered the business side of science, she...

20. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type

    Blog Post

    Published

    March 12, 2020, 1:15 p.m.

    ...promoters across these three species, the Roska lab concluded that a promoter’s ability to target a cell class...