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We narrowed to 856 results for: ELL

Showing: 561 - 580 of 856 results

  1. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

    Type
    Blog Post
    Published
    Jan. 8, 2014, 3:10 p.m.
    ... (iPS) Cells - 2008 was marked by the influx of plasmids for reprogramming differentiated cells into pluripotent... pluripotent stem cells. Plasmids from Shinya Yamanaka's lab and James Thomson's lab were the most frequently...infographic created by our own Addgenie, Laura Veckerelli. Click on the picture below to see the full graphic...

  2. Editor's Choice, August 2016

    Type
    Blog Post
    Published
    Sept. 2, 2016, 1:32 p.m.
    ...Clearly we have all of our writers to thank for the excellent content they’ve churned out in the last month....contributed by guest blogger Kristian Laursen from Cornell University, is a bit old school and provides a ...or G->A transitions in a target sequence in live cells. This technique uses catalytically inactive CRISPR...and other means of generating mutations in live cells are all discussed further in this informative post...

  3. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    Published
    Jan. 8, 2015, 2:34 p.m.
    ...down to one, key concept: Creating a culture of excellent, effective communication between all members of...ensuring that the group always conducts exceptionally well-managed meetings. Members face one another, and...engagement in the issues at hand and eagerness to get, as well as give, information to make improvements. Members...meetings with team members (don’t become known for cancelling these); ask for feedback and input on multiple...

  4. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    Published
    April 27, 2017, 2:30 p.m.
    ... the News Chapter 1: Introduction - Vini and Amy tell us a little about themselves. Chapter 2: What is...brings in scientists from a variety of fields as well as students from the ed school, design school, and...context. Episodes are driven by narrative and story telling. Chapter 5: The SITN Blog - Vini and Amy describe...scientific information for the general public as well as a platform for scientists to be viewed as people...

  5. "What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring

    Type
    Blog Post
    Published
    Dec. 17, 2013, 2:47 p.m.
    ...goals/needs/situation in mind, rather than (or as well as) their own.” “Someone to act as your ‘cheerleader...Apparently, a good Jedi does not necessarily an excellent mentor make. Are your current mentors/advisors...concrete goals and helps you reach your goals Listens well and communicates clearly and openly Gives honest...What Kind of Mentor Do I Want To Be?” In this excellent essay Acclimatrix, an assistant professor at a...

  6. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    Published
    Dec. 5, 2019, 1:49 p.m.
    ...make-over as well. While in the course of redesigning the homepage, and striving to tell our story in ...navigation, while also updating brand messaging, as well as simplifying the page, and making it all look ...content to dictate the space. We wanted to comfortably tell our story, to get scientists excited about what ...

  7. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    Published
    April 1, 2014, 5:33 p.m.
    ...we'd like to extend a special thank you to Dr. Tom Ellis (Imperial College London), Dr. Connie Cepko (Harvard...asked leading professors for their top 3 tips. Tom Ellis 1. Keep up with the literature. He recommends setting...mentees who have goals outside of the university? Ellis recommends that a student interested in leaving ...Acknowledgments Thank you again to Connie Cepko, Tom Ellis, and George Church for answering our burning questions...

  8. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ... GFP, enabling you to select cells with high GFP expression. These cells have the highest levels of Cas9... on the same plasmid, you’ll make sure that each cell that gets the plasmid contains all of the desired...gRNA, which causes hypopigmentation, thus marking cells that have undergone genomic modification. In this..., et al. “Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system.” Scientific...

  9. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    Published
    June 19, 2018, 1:38 p.m.
    ...Signal/Imaging: Fluorescent reporters are not as well suited for imaging organs located deep in the body...long vesicles have hollow interiors and a protein shell that excludes water but is gas permeable. These ...These vesicles are primarily used by water-dwelling photosynthetic bacteria and archaea to regulate their ...limit of detection for arg1 expressing ECN is 109 cells/ml, which is within the range of  the ~1011 commensal...

  10. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...antigens in living cells or tissues. sdAbs can also be used to manipulate cellular functions, like in ...sdAbs over conventional antibodies include:  Excellent tissue penetration, thanks to their small size... their way out of the lab and into the clinic as well. The first sdAb based therapeutic was approved in...

  11. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    Published
    April 25, 2024, 1:15 p.m.
    ...tangential subjects, and let curiosity guide your intellectual journey. Practical approaches One approach...outdoors in nature or visiting museums can be excellent methods to detach from the constant stream of ...unlocking the next frontier of knowledge. Matteo Tardelli authored the book "Beyond Academia: Stories and...molecular biology and metabolism. He now guides fellow PhDs in their careers with actionable steps and...

  12. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

    Type
    Blog Post
    Published
    June 11, 2019, 1:16 p.m.
    ...human body, cellular biology, medical specialities, and general items. Individual images as well as collections...website has many tutorials and Lynda has a tutorial as well, which you may have access to through your university...discussed? Or know of a tool or resource not mentioned? Tell us about it in the comments section!  Additional...

  13. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    Published
    Sept. 12, 2023, 1:15 p.m.
    ... repository of CRISPR screens Cellosaurus: A knowledge resource for cell lines IBEX Knowledge Base: A ...publications and commercially available sources, as well as those deposited with Addgene. NCBI BLAST: Compares... stand-alone sites for general primer design, as well as primer design for different cloning applications...

  14. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    Published
    May 2, 2023, 1 p.m.
    ...50% of predicted genes have unidentified roles as well (Vanni, et al). Is it true that this many genes ...fluorescent protein (GFP) was the reporter gene and green cells were observed in the kidneys at a specific developmental...genes of interest may not be expressed in the tissue/cell line you are working with. Development-specific...10.7554/eLife.67667 Springer, S. P. Gene Traps. Plant Cell. (2000) 12(7): 1007-1020. DOI: 10.1105/tpc.12.7.1007...

  15. 10 Ways to Share Your Science!

    Type
    Blog Post
    Published
    May 13, 2014, 8:34 p.m.
    ...scientists to get out there and talk about their work. To tell others why that work is cool and why it really matters...seem all that interested in the details, work on telling it another way. You’ll know you are on the right...Plan to write regularly and, when you are ready, tell your friends or others in the field where to find... science writing, look into a AAAS Mass Media Fellowship or maybe an internship at a place like Science...

  16. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ...devise new strategies to reproduce within a host cell. A few virus particles have learned to circumvent...deliver a highly specific, programmable platform well-suited for various innovative biological and translative...2011 Jun; 9, 467-477. Bondy-Denomy J, Pawluk A, Maxwell KL, Davidson AR.  Nature. 2013 Jan; 493, 429–432...): 819–23. Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville JE, Church GM. Science. ...

  17. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type
    Blog Post
    Published
    June 27, 2018, 1:16 p.m.
    ...Nanobody toolkit to monitor retrograde transport at the cell surface Article contributed by Marcy Patrick  Listen...nanobodies to study retrograde transport from the cell surface. Their toolkit includes a set of plasmids...new technologies to quantify proteins in tissues, cells, and biofluids. Recently, Sam Myers, a Scientist...an sgRNA expressed from a separate plasmid. When cells are treated with biotin–phenol and H2O2, APEX2 labels...

  18. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ... useful and productive, forming a different constellation of interests and skills than what fits for applied...Queen” shows how punctuation can be wildly funny as well as financially critical. Those who work on the publishing...outlets (AAAS Mass Media Science & Engineering Fellows Program). Science journalism programs at various...sciences, viewed through the lens of development, cell, and molecular biology. Beyond that, she is also...

  19. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type
    Blog Post
    Published
    Sept. 5, 2019, 1:06 p.m.
    ...closely with TTOs to help protect our depositors’ intellectual property, and to help requesting institutions... research; provide specific examples if possible Tell them about the Golden Stopwatch! Any organization...University  Princeton University  Purdue University Rockefeller University  Salk Institute for Biological Studies...agreement Technology Transfer: the exchange of intellectual property (as inventions, ideas, plasmids) among...

  20. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ...thermoplastic polymers that can work with all the bells and whistles. The fibers are made from a process...later! Next, additional components are added - the ‘bells and whistles’ – including microscale light emitting...of the stiffness the fibers would need to be, as well as other features necessary for implantation in ...and chemically excited a class of enteroendocrine cells within the intestines known as neuropods. They observed...
Showing: 561 - 580 of 856 results