Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 930 results for: Gene

Showing: 561 - 580 of 930 results

  1. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...IPTG. T7 RNAP is then available to transcribe the gene of interest from a T7 promoter on the plasmid. Many...::T7 gene1 [lon] ompT gal sulA11 R(mcr-73::miniTn10--TetS)2 [dcm] R(zgb-210::Tn10--TetS) General protein...cytosine within a particular sequence. Browse Addgene's curated list of Bacterial Expression Systems Table...DE3) F- ompT lon hsdSB (rB- mB-) gal dcm (DE3) General protein expression BL21 (DE3) pLysS* Chloramphenicol...ompT lon hsdSB (rB- mB-) gal dcm rne131 (DE3) General expression; not recommended for toxic proteins ...lon hsdSB (rB- mB-) gal dcm araB::T7RNAP-tetA General protein expression  BLR (DE3) Tetracycline RecA-deficient...overexpression? The strains described above should generate sufficient expression levels for most purposes...

  2. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    Published
    April 9, 2019, 1:13 p.m.
    ...mediated HDR) enables the tagging of endogenous genes close to native expression levels. However, it is...chimera are encoded by DNA. Since researchers can generate almost any DNA sequence in the way that they like...proteins is relatively straightforward. However, generating a fusion while keeping all of the native properties...challenging. In this blog I discuss strategies to generate fusion proteins and highlight some aspects of ... from Aequorea victoria and its variants are genetically encoded fluorescent probes. One of the limitations...restriction enzymes in the multiple cloning site for generating fusions. For the POI-fluorescent protein fusions...sufficient. For the fluorescent protein-POI fusions, we generate straight fusions, without any linker. When both...

  3. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...achieved through, for example, a previously induced gene knockout. Besides amine- and DNA-binding dyes, nucleoside... on Addgene.org Ready-to-use recombinant antibodies at Addgene Addgene's Antibody Guide Addgene's Antibody... combines a marker with a signal. For clarity, Addgene uses 'labeling' to refer to antibodies and 'staining...Find streptavidin plasmids and biotin plasmids at Addgene!     Figure 1: Indirect detection of target...signal. Dump gating When you are working with heterogeneous cell mixes, like bulk lympho-/splenocytes, you...Antibody Plasmid Collection Resources on the Addgene blog Antibodies 101: Reading a Flow Plot Antibodies...

  4. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...do not naturally express the protein or have had gene expression knocked down or knocked out. If you are...antibodies Resources on Addgene.org Find written and video protocols Visit Addgene's antibodies collection...plasmid encoding your protein of interest from Addgene, transfected it into your target cells, now what...wet, semi-dry, and dry but all follow the same general principle where the gel and membrane are “sandwiched...may even find plasmids encoding antibodies from Addgene that you can make on your own.  To narrow your ... found this blog helpful be sure to check out Addgene’s blog for overviews of other antibody topics. ...10.4103/1947-2714.100998 Additional resources on the Addgene blog Find other tips and tricks for molecular ...

  5. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...which come from naturally-occurring immunoglobulin genes, recombinant antibodies are plasmid-based. Their... What is an Antibody? Resources on Addgene.org: Browse Addgene’s Antibody Plasmid Collection Find antibodies...cascades that clear these infections. Anything that generates an antibody response in the immune system is referred...in the DNA of the hybridoma can occur, known as genetic drift, meaning that over time, the antibody you...monoclonal antibodies, recombinant antibodies are a homogeneous population. However, unlike monoclonal antibodies...antigen binding, the variable regions alone can be generated as a fusion protein. An ScFv is made up of the...detect the presence of a single protein from a heterogeneous mixture - for example, cell lysate or media ...

  6. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ... 18 lung cancer patients. This study used CRISPR gene editing to knockout PD-1 on immune cells harvested... Cell heterogeneity Tumors are composed of a wide variety of cell types. This cell heterogeneity is an...cells that escape the elimination stage acquire genetic alterations and alter cell-surface antigen production... promotes the production of VEGF to increase angiogenesis around the tumor. This in turn regulates the... immunotherapy. Using activated DCs ex vivo to generate cytotoxic effector T cells, and targeting specific...played a huge role. For instance, researchers are genetically altering tumor cells and immune cells to knockout...element of successful tumor progression. A tumor’s genetic composition is constantly in flux and is dependent...

  7. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...proteins or cloning backbones to study your gene of interest with these tools. Conclusion Researchers and... Ligands GCE4All: Making Genetic Code Expansion Accessible Addgene's Genetic Code Expansion Collection... & Small Molecule Fluorophores. Addgene Blog. https://blog.addgene.org/better-dyeing-through-chemistry-small-molecule-fluorophores... is now the most popular self-labeling tag in Addgene’s collection. CLIP-tag: Also in 2008, Kai Johnsson...specific with a small and minimally-disruptive genetic tag on the protein of interest. Table 1: Summary...are the most popular self-labeling enzymes in Addgene’s collection, so you may be able to get a plasmid...ligand to use for your experiment, and explore Addgene's collection to find self-labeling fusion proteins...

  8. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...procedure and application to the major ALS/FTD disease gene C9ORF72. eLife, 8, e48363. https://doi.org/10.7554...101: Validation Resources on addgene.org Addgene's Antibody Guide Addgene's Antibody Protocols... outlined below. Knockout cell line approach Genetic knockout-based validation can conclusively prove...you are working with cells that are amenable to genetic knockout, this represents a very robust approach...to start with a screening procedure in easily genetically modified cell types (top). The specific approach...10.1038/s41598-019-55133-7 More resources from the Addgene blog Introduction to Gating in Flow Cytometry Antibodies...

  9. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ...transition from weak, random binding to precision gene editing requires three major steps: RNA loading,...resources on addgene.org  Addgene's CRISPR Guide Addgene's CRISPR 101 eBook  Resources on the Addgene blog Components...from Streptococcus pyogenes.   Figure 2:  Crystal structure of S. pyogenes Cas9 in the apo state...    Figure 3: Crystal structure of S. pyogenes Cas9 bound to guide RNA (PDB ID 4ZT0), shown opaque...sequence isn’t in place yet.     Figure 4: S. pyogenes Cas9 in the apo state (PDB ID 4CMP, top) compared... residues in the CTD can get cozy with DNA. S. pyogenes Cas9 recognizes the PAM sequence 5’-NGG-3’, where...complementary strand is mismatched.   Figure 5: S. pyogenes Cas9 bound to guide RNA and target DNA (PDB ID...

  10. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    Published
    Nov. 3, 2015, 3:30 p.m.
    ...of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation." Nature biotechnology (2014). PubMed...to select a tool based on your project and not general opinion. I could spend a lot of time running through... Online tools dominate the software space but generally rely on less powerful algorithms to detect off-targets...tools available mostly work on any computer but generally require working knowledge of the command line ...tools require some kind of data input in order to generate results. While different types of input are more...focus on specific organisms. For this reason I generally recommend that laboratories that have already ...PubMed Central PMCID: PMC4262738. Resources at Addgene Get Tips on How to Design a gRNA Read Our CRISPR...

  11. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...). Efficient site-specific integration of large genes in mammalian cells via continuously evolved recombinases...Over 75,000 pathogenic genetic variants have been identified in humans and cataloged in the ClinVar database...editing tools. We’ll summarize the major ones in Addgene’s repository later in the post. Advantages of prime...by double strand breaks has been widely used to generate precise changes. However, the efficiency of Cas9...Prime editing has a lot of promise for treating genetic diseases. In our summer Hot Plasmids post, we covered...demonstration of prime editing used to rescue a genetic disease phenotype in an animal model, which was...s41587-022-01527-4 Additional resources on the Addgene blog PRIDICT: Predicting Efficiencies of Prime...

  12. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...Resources on Addgene.org Addgene’s Viral Services Addgene’s Virus Protocols Addgene’s Chemogenetics Guide ...system.   Figure 2: General overview of chemigenetic tools. A genetically encoded component is delivered...Resources on the Addgene blog Viral Vectors 101: Chemogenetics Viral Vectors 101: Optogenetics Fluorescent... within a cell are genetically encoded voltage indicators (GEVIs) and chemigenetic sensors. These tools...on this later). Chemigenetic sensors take a hybrid approach and utilize both a genetic component as well... system. Single Fluor Chemigenetic Sensors This system consists of a genetically expressed enzyme, typically...about how viruses can be used for chemigenetics, biosensors, and generally as biological tools, our blog ...

  13. New Viral Vectors - Winter 2025

    Type
    Blog Post
    Published
    Jan. 28, 2025, noon
    ...AAV1 Chemogenetics Scott Sternson New viral prep pAAV-CaMKIIa-hM3D(Gq)-mCherry AAV1 Chemogenetics Bryan...mCherry AAV1 Chemogenetics Bryan Roth New serotype pAAV-hSyn-hM3D(Gq)-mCherry AAV1 Chemogenetics Bryan Roth... Brian O'Neill.  More resources on the Addgene blog Addgene's Data Hub What is a Virus (and a Viral Vector...but browsing the 49 new preps available in the Addgene viral vector repository with a nice cup of hot ...Table 1: New ready-to-use viral vectors in the Addgene repository  Name Serotype Category Depositor... serotype pAAV-hSyn-hM4D(Gi)-mCherry AAV1 Chemogenetics Bryan Roth New serotype pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato...prep pAAV_hSyn1-SIO-stGtACR2-FusionRed AAV5 Optogenetics Ofer Yizhar New serotype pAAV-CaMKIIa-ChRmine-oScarlet-KV...

  14. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...learn what Addgene can do to help. More resources on the Addgene blog Twenty Years of Addgene Sharing: ...researchers around the globe. Addgene’s educational resources As Addgene built out our scientific support...other videos on Addgene’s YouTube channel.   Figure 4: Global viewership of the Addgene YouTube channel...about virus, I learned from Addgene,” or “I learned to clone from the Addgene website.” We delight in seeing...When Addgene opened in 2004, we focused on creating a repository that made it easy to share plasmids,...accessible, due to a wide range of logistical barriers. Addgene worked to identify, reduce, and remove these logistical...accessible weren’t always the same thing, shaped both Addgene’s founding and our growth into the company we are...

  15. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...Community Addgene: In 2012 your lab first deposited plasmids with Addgene. In 2014, you joined Addgene's Advisory...lab. In June 2014, Dr. Fujii joined Addgene's Advisory Board.  Addgene: Your lab has worked extensively ... enChIP through Addgene. Retroviral Expression System for enChIP using CRISPR Addgene: Your lab recently...the plasmids of these systems to Addgene in the near future. Addgene: Has your lab been working on any...first knew Addgene around 2010 and found the scheme very nice at first sight in that Addgene takes care...ourselves without Addgene. So, when I received an invitation to join the Advisory Board of Addgene, I accepted...Hodaka Fujii lab's Addgene page and click the "Subscribe to Alerts" link in the Addgene Alerts box. References...

  16. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...expected to grow with the ever-expanding genetic toolbox generated by the continuous effort of numerous scientists...). GFP is the most popular, most widely used genetically encoded fluorescent probe. Several factors contribute...probe, it is the first choice when selecting a genetically encoded fluorescent tag. There are, however, ...1998). Another mutation (A206K) is necessary to generate the strictly monomeric EGFP variant, mEGFP (Zacharias..., followed by an intramolecular reaction that generates the chromophore (Tsien, 1998). Importantly, this...Thorn, 2017). These fluorescent analogues are generally added to the bathing solution and require uptake...adding GFP may alter diffusion rates. Smaller genetically encoded probes are based on cofactor binding ...

  17. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...If you’ve ordered a pooled library from Addgene, you may have been a little surprised at how this powerful...deposited many types of pooled libraries here at Addgene, including cDNA, barcoding, screening, and gRNA...gRNA libraries. Most of the pooled libraries at Addgene are lentiviral pooled libraries, though other types...types exist.  When you buy a pooled library from Addgene, you can either order a DNA pooled library, which...single tube, or a pre-made viral prep library. Addgene’s pooled libraries are typically DNA preps, but ... A) Amplification, validation, and lentiviral generation of a pooled library. An aliquot of the pooled...experiments. Pooled library lentiviral preps are generally aliquoted to reduce the number of freeze-thaw ...

  18. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type
    Blog Post
    Published
    Sept. 5, 2019, 1:06 p.m.
    ... institution’s TTO or legal office first. Generally, Addgene is also waiting to receive that legal agreement...materials from Addgene can qualify. We also reward improvement against one’s own record Mention Addgene Master...question? Refer them to Addgene’s Tech Transfer Team: techtransfer@addgene.org Congratulations to the...between a TTO and Addgene, whose terms apply to all future deposits of materials with Addgene Master Reception...between a TTO and Addgene, whose terms apply to all future materials requests from Addgene MTA: material ...commercialization Additional resources on Addgene.org Addgene Help Center: Tech Transfer Technology Transfer...arrival of the newest plasmid you requested from Addgene...the plasmid that will help you run that last ...

  19. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ... Resources on Addgene.org Learn how Addgene engages with the community on our Addgene gives back page...Initiatives for the Personal Genetics Education Project (pgEd). Advances in genetic technologies and other ...broadens the conversation about genetics pgEd, based at the Department of Genetics of Harvard Medical School...existing inequalities and injustices. The Personal Genetics Education Project (pgEd), where I currently work...work, strives to broaden the conversations about genetics and facilitate dialogue between scientists and...the personal, social and ethical implications of genetics. In these conversations, pgEd strives to be inclusive...and empowered to make their own decisions about genetics, both as individual consumers of technologies ...

  20. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...Resources at Addgene.org Find pooled libraries at Addgene Find basic lab protocols at Addgene ...As a tech at Addgene, part of my job, in addition to preparing DNA and generating/replenishing kits, ...information from the workshop. Future workshops for Addgene Addgene strives to be a great resource for the research...Technician at Addgene who is interested in medicine and music.       Additional Resources on the Addgene Blog ... use what we learn to improve processes in the Addgene lab. In this post, I’ll tell you a little bit about...excited to go there because both LabCentral and Addgene aim to make scientific advancement easier. NEBNext...extract libraries from previously fragmented DNA.The general process for library preparation using this kit ...
Showing: 561 - 580 of 930 results