We narrowed to 893 results for: IND

...recommend avoiding a High Quantity/Low Quality mindset for any applications. DNA quantity is fairly self...cellular debris look very similar. However, keep in mind that kit components (i.e. buffers, columns, or beads...columns or magnetic beads. Both approaches involve binding the plasmid DNA from your cleared lysate to a substrate...specifically designed with environmental sustainability in mind (Table 2). Table 2: Sustainability features highlighted...instruction manual will help explain the rationale behind each step and provide troubleshooting advice. In...

...the paper is published, while giving you peace of mind that your materials will only be made public when...will help us ensure that plasmids don’t get left behind in this status — a win-win situation for all. ...distribution instead of risking leaving them on hold indefinitely. Of course, the fastest way for us to release...out as planned, but you still hope somebody will find the plasmids useful, we’re happy to distribute unpublished...

...nuclease-deficient CRISPR-Cas system identifies and binds a genomic target using a gRNA, and an affiliated...was able to integrate DNA payloads of up to 15 kb. Find evoCAST plasmids here! The new horizons of evoCAST...tailored treatments to specific mutations require individualized optimizations, safety testing, and approval...approach. evoCAST is an important step toward that kind of gene treatment. The researchers also compared...

...Cellosaurus STR similarity search tool (CLASTR) to find cells lines similar to your STR profiles of interest...who knows where), now is as good a time as ever to find out the true identity of the line? Once you’re run...Contributing to Cellosaurus Generated a new cell? Or finding errors in the available information about a cell...over globe, with some surprising, and important, findings being shared. For instance, recently Cellosaurus...

...the Molecular Biology Reference Find protocols on plasmid cloning Find plasmids for your research ...temperature for a short period, and there are indications that Tris buffer is better than water in these...1874070701307010010 Additional resources on the Addgene blog Find five ways to purify DNA without a kit Read our blog...

...companies can recycle plastic-- you just have to find them (or keep reading). Corning takes back and recycles...take to action! These days, it’s important for individuals, companies, and universities to be sustainability... the Addgene blog Subscribe to the Addgene blog Find lab tips for your research Read another blog post...other scientists save resources by sharing plasmids Find plasmids at Addgene ...

...January. This involves going in front of a camera and kind of walking them through the steps of, for example... says Caitlin. Caitlin also works to meet the individual needs of schools interested in using eScience...what I did as an SME, and then 75% of the time I’m kind of the go-to person for my department.” Caitlin ...Addgene podcast with educator and science rap mastermind Tom McFadden Get tips about management for scientists...

...screening system and plans to employ the light‐inducible bacterial expression system pDawn to clone and...for their potential agricultural and medical use. Find More Optogenetic Tools Like pDawn  Making cassava...supplements Dr. Mrinalini Nair, University of Baroda, India Dr. Mrinalini Nair, Associate Professor (Right),...aims to leverage a glycine oxidase reaction to indirectly detect glyphosate via H2O2, one of the reaction...

...Addgene.org Find Fluorescent Protein Empy Backbones Visit Our Fluorescent Protein Guide Page Find Luciferase...more is often better. More transcription factor binding sites in a promoter lead to higher transcriptional...the power of SunTag for single molecule imaging, finding that plasma membrane-targeted CAAX-SunTag was 18...

...Resources on Addgene.org Start a Deposit Find Additional Hot Plasmids Find the Full List of Blue Flame Depositors...level system on our website:   A yellow flame indicates that a plasmid has been requested 20+ times   ...Blog Check Out the Top Requested Plasmid in 2015 Find Hot Plasmid Technologies Write about Your Favorite...

...with CRIPSR Resources on Addgene.org Find ACVR1 Plasmids Find Plasmids for Disease Research on OurSpecial...and awareness for rare disease and helps patients find the correct treatments, if available. As part of...commercially developed therapeutic antibody that binds Activin A and interferes with inappropriate activin-ACVR1...

... bench work. To really be happy, you may need to find a way to do, manage and lead all in one day.  How...is the ability to assess and initiate things independently. Taking initiative doesn’t mean going rogue,...listed a few of my favorites. Additionally, you can find more suggested resources on this topic and get the...Articles on Leadership, also can be purchased individually) ...

...Just as physical barcodes help librarians track individual books, genetic barcodes allow researchers to ... town! Pooled libraries can be designed for all kinds of different high-throughput experiments. Our most...species. This approach is useful for screening for binding properties. For example, several coronavirus libraries...teaching us more about viral evolution and antibody binding. Figure 3: Schematic of phage display screening...

...Resources on Addgene.org Find AAVs at Addgene Learn more about Addgene's viral service Find protocols for working...tagmentation-based library preparation procedures. This finding eliminates the double-stranding step and reduces... Lecomte E, Tournaire B, Cogné B, Dupont J-B, Lindenbaum P, Martin-Fontaine M, Broucque F, Robin C, Hebben...

...scientists using Addgene AAV viral preps to easily find and share useful technical data. These data focus...usage of these tools, which aren’t always easy to find in publications, so this is a much-needed community..., scientists can use the AAV Data Hub to quickly find details they need for planning their next experiments....org Read our AAV guide Browse all  AAV vectors Find ready-to-use AAV preps ...

...and visualize. For more details on this procedure, find the protocol here.   Tips and troubleshooting ...Super-resolution mapping of neuronal circuitry with an index-optimized clearing agent." Cell reports14.11 (2016...Meng-Tsen, and Takeshi Imai. "Optical clearing and index matching of tissue samples for High-resolution fluorescence...viral service See our fluorescent protein collection Find AAVs at Addgene ...

... but did you know that Addgene also performs independent research? Since launching the viral service in...cells treated with sucrose or other osmolytes to induce hypertonicity display enhanced monoclonal antibody...blog Read our beginner's guide to viral vectors Find all of our viral vector blog posts Download our ...Addgene's viral service Learn about viral production Find viral protocols here ...

... try to be as chronological as possible — if you find yourself jumping backwards in time to explain something...your lab or science or your CV has grown as well. Find your audience and fit them You don’t need to explain... paper. Build lots of versions of your story and find the one that fits your audience. Enjoy the story...Check out our blog posts on science communication Find interviews scientists in SciComm careers Check out...

...can always head over to the Viral Service page to find the full list of what’s available or suggest additional...beginners just starting work with virus. You can find all of our viral vector blog posts here. If you ...    www.addgene.org On the Addgene website you’ll find our updated viral vector pages as well as expanded...Addgene.org Overview of Viral Production at Addgene Find Virus Protocols Browse All Viral Vectors ...

...potentially rebind and nick the newly ligated DNA. Salt helps prevent topoisomerase I from rebinding, which...the "A" gets added to all PCR products. Keep in mind that Taq polymerase has an error rate of about 1...to Verify Your Plasmid Resources on Addgene.org Find More Plasmid Cloning Protocols Catch Up On Your ...