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We narrowed to 878 results for: IND

Showing: 561 - 580 of 878 results

  1. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...short-hand for Clustered Regularly Interspaced Short Palindromic Repeats, in Pubmed returned 728 articles (3/12...because the Cas9 nickase doesn't have the ability to induce double-stranded breaks like the wildtype Cas9 does...single-cell colonies, grow them up, then perform individual genotyping (using Sanger sequencing, for example...frequently requested CRISPR plasmids at Addgene Find protocols and gRNA design tools: List of CRISPR...

  2. The Strength of Story Telling

    Type
    Blog Post
    Published
    Dec. 19, 2017, 2 p.m.
    ... try to be as chronological as possible — if you find yourself jumping backwards in time to explain something...your lab or science or your CV has grown as well. Find your audience and fit them You don’t need to explain... paper. Build lots of versions of your story and find the one that fits your audience. Enjoy the story...Check out our blog posts on science communication Find interviews scientists in SciComm careers Check out...

  3. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    Published
    Jan. 24, 2019, 2:24 p.m.
    ...and visualize. For more details on this procedure, find the protocol here.   Tips and troubleshooting ...Super-resolution mapping of neuronal circuitry with an index-optimized clearing agent." Cell reports14.11 (2016...Meng-Tsen, and Takeshi Imai. "Optical clearing and index matching of tissue samples for High-resolution fluorescence...viral service See our fluorescent protein collection Find AAVs at Addgene ...

  4. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    Published
    Dec. 13, 2018, 1:14 p.m.
    ... but did you know that Addgene also performs independent research? Since launching the viral service in...cells treated with sucrose or other osmolytes to induce hypertonicity display enhanced monoclonal antibody...blog Read our beginner's guide to viral vectors Find all of our viral vector blog posts Download our ...Addgene's viral service Learn about viral production Find viral protocols here ...

  5. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    Published
    April 23, 2020, 1:15 p.m.
    ...Resources on Addgene.org Find AAVs at Addgene Learn more about Addgene's viral service Find protocols for working...tagmentation-based library preparation procedures. This finding eliminates the double-stranding step and reduces... Lecomte E, Tournaire B, Cogné B, Dupont J-B, Lindenbaum P, Martin-Fontaine M, Broucque F, Robin C, Hebben...

  6. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    Published
    March 5, 2020, 2:15 p.m.
    ...scientists using Addgene AAV viral preps to easily find and share useful technical data. These data focus...usage of these tools, which aren’t always easy to find in publications, so this is a much-needed community..., scientists can use the AAV Data Hub to quickly find details they need for planning their next experiments....org Read our AAV guide Browse all  AAV vectors Find ready-to-use AAV preps ...

  7. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    Published
    Nov. 17, 2016, 3:30 p.m.
    ...can always head over to the Viral Service page to find the full list of what’s available or suggest additional...beginners just starting work with virus. You can find all of our viral vector blog posts here. If you ...    www.addgene.org On the Addgene website you’ll find our updated viral vector pages as well as expanded...Addgene.org Overview of Viral Production at Addgene Find Virus Protocols Browse All Viral Vectors ...

  8. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...potentially rebind and nick the newly ligated DNA. Salt helps prevent topoisomerase I from rebinding, which...the "A" gets added to all PCR products. Keep in mind that Taq polymerase has an error rate of about 1...to Verify Your Plasmid Resources on Addgene.org Find More Plasmid Cloning Protocols Catch Up On Your ...

  9. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...Hybridization Sequence: The region of the primer that binds to the sequence to be amplified (usually 18-21bp... we want to amplify and design primers that will bind to and replicate it. The following image shows the...5'-ATGTGGCATATCTCGAAGTAC-3' for the region that binds the ORF and we will add the EcoRI restriction site... Reference Pages Deposit Your Completed Plasmids Find the Perfect Plasmid for Your Research ...

  10. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ...they added flanking loxP sites and a heat-shock inducible Cre to generate a self-excising cassette (SEC)...genetics.115.178335. [Epub ahead of print] PubMed. Find plasmids from this publication at Addgene. Other...: 10.1038/nmeth.2641. Epub 2013 Sep 1. PubMed. Find plasmids from this publication at Addgene. Scalable...genetics.114.170423. Epub 2014 Sep 23. PubMed. Find plasmids from this publication at Addgene. Additional...

  11. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    Published
    March 6, 2014, 7:12 p.m.
    ...having a mentor, developed some strategies for finding mentors and, perhaps, asked someone to support ...identified. Keep a journal to record topics, plans, findings, and progress over the course of an ongoing mentoring...Forced Choice Analysis is a fun example and you can find it in our Mentoring for Scientists Guide.  You can...steps (e.g. Meet 10 PhDs with interesting careers, find a summer internship, etc.). It is helpful to work...

  12. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ... my head and left me thinking: I wish I had this kind of experience earlier! After all, students build...PMID: 30083609. PubMed Central PMCID: PMC6070313. Find the plasmids from this publication at Addgene! ... Additional Resources on the Addgene Blog Find planarian tools for teaching developmental biology Use... Check out our molecular biology reference pages Find plasmids from the BioBits BrightTM publication here...

  13. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

    Type
    Blog Post
    Published
    July 21, 2017, 12:48 p.m.
    ...or off. Gal4 is a transcriptional activator that binds to UAS enhancer sequences found in DNA. It then ...recruits transcription machinery to the site to induce gene expression. Thus, genes and siRNA encoded ...neuronal compartment, you could express them individually or simultaneously. Do note for control purposes... much protein as overexpression of each gene individually. Applications of the Gal4/UAS system There are...

  14. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    Published
    Sept. 12, 2019, 1 p.m.
    ...Learn about Addgene's viral service Find viral protocols and videos Find AAVs for your research ...serotypes, since these 20 ul samples are distributed individually. See our Serotype Testing AAV inventory for ...Addgene blog Read all of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors...

  15. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ... Normally, β10-11 are antiparallel. To prevent binding, the β strands in FlipGFP were redesigned so that...cells expressing FlipGFP with staurosporine, which induces apoptosis by activating caspase-3, and detected...understand these diseases and disorders. You can find the plasmids for FlipGFP and FlipCherry at Addgene...Addgene.org Browse the fluorescent protein collection Find empty backbones for fluorescent protein fusions ...

  16. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ...Learn about Addgene's viral service Find viral protocols and videos Find AAVs for your research ...scientists have yet to decipher the mechanistic aspects behind these improvements. What have exosome AAVs achieved...Addgene blog Read all of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors...

  17. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ...motivated to do anything and everything we can to help find a cure -- and quickly. Normally drug discovery is...and help us figure out what is going on there!).  Find the Krogan Lab SARS-CoV-2 plasmids (Note: The Nsp6...over the world, as far flung as the EU, Russia, India and Saudi Arabia -- as well as closer to home in...QBI_UCSF and KroganLab), Instagram, and Facebook. Find more COVID-19 and coronavirus related plasmids and...

  18. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ... monogenetic diseases, the bulk of which you can find below. CRISPR for complex genetic diseases As CRISPR...single-guide RNA targeting the mutant allele (7). An independent team led by Hans Clevers at Hubrecht Institute...fibrosis report. “This is a big advantage over induced pluripotent stem cells, which need to go through...affects multiple organ systems. Nevertheless, the findings are proof-of-principle for use of CRISPR to correct...

  19. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ...CD8 or CD4 epitopes, as well as their potential binding affinity to major histocompatibility complexes ...not yet possible for these algorithms to model individually-specific factors, such as previous exposures...prediction of immunogenicity is to validate the findings from the in silico assessments, as well as analyze...dentritic cells or macrophages through cytokine induction (Groell et.al, 2018). Thus, microscopic in-vitro...

  20. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    Published
    April 28, 2021, 1:15 p.m.
    ... has a web and desktop version that works with Windows, Mac, and Linux operating systems.  Zotero  Zotero...desktop based program that’s compatible with Mac, Windows, and Linux.  Mendeley and Zotero have a lot of ...two citation managers. If you want more tips on finding and organizing papers, check out this related blog... blog post: Early Career Researcher Toolbox: Finding Relevant Papers. These are only a few of the many...
Showing: 561 - 580 of 878 results