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We narrowed to 889 results for: IND

Showing: 561 - 580 of 889 results

  1. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    Published
    June 4, 2020, 1:22 p.m.
    ...the Molecular Biology Reference Find protocols on plasmid cloning Find plasmids for your research ...temperature for a short period, and there are indications that Tris buffer is better than water in these...1874070701307010010 Additional resources on the Addgene blog Find five ways to purify DNA without a kit Read our blog...

  2. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    Published
    June 24, 2025, 1:15 p.m.
    ...the paper is published, while giving you peace of mind that your materials will only be made public when...will help us ensure that plasmids don’t get left behind in this status — a win-win situation for all. ...distribution instead of risking leaving them on hold indefinitely. Of course, the fastest way for us to release...out as planned, but you still hope somebody will find the plasmids useful, we’re happy to distribute unpublished...

  3. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    Published
    June 10, 2025, 1:15 p.m.
    ...nuclease-deficient CRISPR-Cas system identifies and binds a genomic target using a gRNA, and an affiliated...was able to integrate DNA payloads of up to 15 kb. Find evoCAST plasmids here! The new horizons of evoCAST...tailored treatments to specific mutations require individualized optimizations, safety testing, and approval...approach. evoCAST is an important step toward that kind of gene treatment. The researchers also compared...

  4. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    Published
    Feb. 21, 2023, 2:15 p.m.
    ...Cellosaurus STR similarity search tool (CLASTR) to find cells lines similar to your STR profiles of interest...who knows where), now is as good a time as ever to find out the true identity of the line? Once you’re run...Contributing to Cellosaurus Generated a new cell? Or finding errors in the available information about a cell...over globe, with some surprising, and important, findings being shared. For instance, recently Cellosaurus...

  5. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

    Type
    Blog Post
    Published
    Nov. 1, 2017, 2:04 p.m.
    ...January. This involves going in front of a camera and kind of walking them through the steps of, for example... says Caitlin. Caitlin also works to meet the individual needs of schools interested in using eScience...what I did as an SME, and then 75% of the time I’m kind of the go-to person for my department.” Caitlin ...Addgene podcast with educator and science rap mastermind Tom McFadden Get tips about management for scientists...

  6. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

    Type
    Blog Post
    Published
    Nov. 14, 2016, 3:30 p.m.
    ...Resources on Addgene.org Start a Deposit Find Additional Hot Plasmids Find the Full List of Blue Flame Depositors...level system on our website:   A yellow flame indicates that a plasmid has been requested 20+ times   ...Blog Check Out the Top Requested Plasmid in 2015 Find Hot Plasmid Technologies Write about Your Favorite...

  7. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    Published
    Sept. 15, 2016, 2:30 p.m.
    ...with CRIPSR Resources on Addgene.org Find ACVR1 Plasmids Find Plasmids for Disease Research on OurSpecial...and awareness for rare disease and helps patients find the correct treatments, if available. As part of...commercially developed therapeutic antibody that binds Activin A and interferes with inappropriate activin-ACVR1...

  8. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    Published
    March 31, 2015, 4:08 p.m.
    ... bench work. To really be happy, you may need to find a way to do, manage and lead all in one day.  How...is the ability to assess and initiate things independently. Taking initiative doesn’t mean going rogue,...listed a few of my favorites. Additionally, you can find more suggested resources on this topic and get the...Articles on Leadership, also can be purchased individually) ...

  9. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    Published
    Jan. 24, 2019, 2:24 p.m.
    ...and visualize. For more details on this procedure, find the protocol here.   Tips and troubleshooting ...Super-resolution mapping of neuronal circuitry with an index-optimized clearing agent." Cell reports14.11 (2016...Meng-Tsen, and Takeshi Imai. "Optical clearing and index matching of tissue samples for High-resolution fluorescence...viral service See our fluorescent protein collection Find AAVs at Addgene ...

  10. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    Published
    April 23, 2020, 1:15 p.m.
    ...Resources on Addgene.org Find AAVs at Addgene Learn more about Addgene's viral service Find protocols for working...tagmentation-based library preparation procedures. This finding eliminates the double-stranding step and reduces... Lecomte E, Tournaire B, Cogné B, Dupont J-B, Lindenbaum P, Martin-Fontaine M, Broucque F, Robin C, Hebben...

  11. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    Published
    March 5, 2020, 2:15 p.m.
    ...scientists using Addgene AAV viral preps to easily find and share useful technical data. These data focus...usage of these tools, which aren’t always easy to find in publications, so this is a much-needed community..., scientists can use the AAV Data Hub to quickly find details they need for planning their next experiments....org Read our AAV guide Browse all  AAV vectors Find ready-to-use AAV preps ...

  12. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    Published
    Dec. 13, 2018, 1:14 p.m.
    ... but did you know that Addgene also performs independent research? Since launching the viral service in...cells treated with sucrose or other osmolytes to induce hypertonicity display enhanced monoclonal antibody...blog Read our beginner's guide to viral vectors Find all of our viral vector blog posts Download our ...Addgene's viral service Learn about viral production Find viral protocols here ...

  13. The Strength of Story Telling

    Type
    Blog Post
    Published
    Dec. 19, 2017, 2 p.m.
    ... try to be as chronological as possible — if you find yourself jumping backwards in time to explain something...your lab or science or your CV has grown as well. Find your audience and fit them You don’t need to explain... paper. Build lots of versions of your story and find the one that fits your audience. Enjoy the story...Check out our blog posts on science communication Find interviews scientists in SciComm careers Check out...

  14. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    Published
    Nov. 17, 2016, 3:30 p.m.
    ...can always head over to the Viral Service page to find the full list of what’s available or suggest additional...beginners just starting work with virus. You can find all of our viral vector blog posts here. If you ...    www.addgene.org On the Addgene website you’ll find our updated viral vector pages as well as expanded...Addgene.org Overview of Viral Production at Addgene Find Virus Protocols Browse All Viral Vectors ...

  15. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...potentially rebind and nick the newly ligated DNA. Salt helps prevent topoisomerase I from rebinding, which...the "A" gets added to all PCR products. Keep in mind that Taq polymerase has an error rate of about 1...to Verify Your Plasmid Resources on Addgene.org Find More Plasmid Cloning Protocols Catch Up On Your ...

  16. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...Hybridization Sequence: The region of the primer that binds to the sequence to be amplified (usually 18-21bp... we want to amplify and design primers that will bind to and replicate it. The following image shows the...5'-ATGTGGCATATCTCGAAGTAC-3' for the region that binds the ORF and we will add the EcoRI restriction site... Reference Pages Deposit Your Completed Plasmids Find the Perfect Plasmid for Your Research ...

  17. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ...they added flanking loxP sites and a heat-shock inducible Cre to generate a self-excising cassette (SEC)...genetics.115.178335. [Epub ahead of print] PubMed. Find plasmids from this publication at Addgene. Other...: 10.1038/nmeth.2641. Epub 2013 Sep 1. PubMed. Find plasmids from this publication at Addgene. Scalable...genetics.114.170423. Epub 2014 Sep 23. PubMed. Find plasmids from this publication at Addgene. Additional...

  18. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    Published
    March 6, 2014, 7:12 p.m.
    ...having a mentor, developed some strategies for finding mentors and, perhaps, asked someone to support ...identified. Keep a journal to record topics, plans, findings, and progress over the course of an ongoing mentoring...Forced Choice Analysis is a fun example and you can find it in our Mentoring for Scientists Guide.  You can...steps (e.g. Meet 10 PhDs with interesting careers, find a summer internship, etc.). It is helpful to work...

  19. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    Published
    Sept. 12, 2019, 1 p.m.
    ...Learn about Addgene's viral service Find viral protocols and videos Find AAVs for your research ...serotypes, since these 20 ul samples are distributed individually. See our Serotype Testing AAV inventory for ...Addgene blog Read all of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors...

  20. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ... Normally, β10-11 are antiparallel. To prevent binding, the β strands in FlipGFP were redesigned so that...cells expressing FlipGFP with staurosporine, which induces apoptosis by activating caspase-3, and detected...understand these diseases and disorders. You can find the plasmids for FlipGFP and FlipCherry at Addgene...Addgene.org Browse the fluorescent protein collection Find empty backbones for fluorescent protein fusions ...
Showing: 561 - 580 of 889 results