We narrowed to 616 results for: LIS;

1. Transferable Skills Guide: Managing a Team

   Type

   Blog Post

   Published

   Jan. 30, 2018, 2:42 p.m.

   ...  Equally as important is to be able to actively listen - this can be hard - but it is the best way to ...

2. 3D Printing Meets CRISPR Cas9

   Type

   Blog Post

   Published

   Oct. 17, 2017, 12:55 p.m.

   ...could make a model of Cas9 based on the structure published by Martin Jinek’s group. We had already made an...

3. Hot Plasmids and Viral Preps - May 2021

   Type

   Blog Post

   Published

   May 18, 2021, 1:15 p.m.

   ...synthetic human scFV library and characterized them by ELISA, homogeneous time resolved fluorescence, and suspension...

4. Cloning Mammalian Cells with the Agarose Method

   Type

   Blog Post

   Published

   Sept. 7, 2017, 12:17 p.m.

   ...protein, we have used Western blots, dot blots, ELISAs, and enzyme assays to select both over- and under-expressing...

5. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

   Type

   Blog Post

   Published

   July 13, 2017, 2:30 p.m.

   ... stocks that are available to everyone. Flybase lists what stocks are available at Bloomington, but you...

6. Addgene's Cancer Collection Pages Connect the Research Community

   Type

   Blog Post

   Published

   Sept. 6, 2016, 2:30 p.m.

   ...gene of interest will direct the investigator to a list of all available Addgene plasmids he or she can ...

7. Plasmids 101: Optimizing Plasmid Yields

   Type

   Blog Post

   Published

   May 26, 2016, 2:30 p.m.

   ...handy guide from OpenWetWare for a more extensive listing of common E. coli strains. Increase the volume ...

8. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

   Type

   Blog Post

   Published

   April 19, 2016, 2:30 p.m.

   ...dimeric FP could ruin your day (and experiment) are listed below. Solutions to avoid these all too common ...

9. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

   Type

   Blog Post

   Published

   May 22, 2015, 4:58 p.m.

   ...negligible level. One major reason we chose to publish the concept and likely capabilities of RNA-guided...

10. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type

    Blog Post

    Published

    June 12, 2014, 3:30 p.m.

    ...purification; the whole cloning process is so simplistic once you have your parts. That was the motivation...

11. Tips for Using BLAST to Verify Plasmids

    Type

    Blog Post

    Published

    May 29, 2014, 1:29 p.m.

    ...alignment tools available. Try these resources for lists of alternatives to BLAST: ExPASy - http://www.expasy.org...

12. Which Fluorescent Protein Should I Use?

    Type

    Blog Post

    Published

    May 20, 2014, 2:06 p.m.

    ...pH changes (e.g. pHluorin, pHTomato). Keep this list handy as you plan your next experiment or to hand...

13. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type

    Blog Post

    Published

    Oct. 10, 2023, 1 p.m.

    ... toxic to dividing cells. The other antibiotics listed below affect continuous cellular processes (e.g...

14. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type

    Blog Post

    Published

    July 16, 2020, 1:15 p.m.

    ...embed_player" overrideable=False, type='scriptV4', hide_playlist=True, viral_sharing=False, embed_button=False...

15. DNA Purification Without a Kit

    Type

    Blog Post

    Published

    Feb. 11, 2020, 2:15 p.m.

    ...adapted from the 1979 Patterson Protocol. When published, the Patterson Protocol was shown to purify DNA...

16. Droplet Digital PCR for AAV Quantitation

    Type

    Blog Post

    Published

    Jan. 16, 2020, 2:18 p.m.

    ... of parameters influencing droplet rain in the Listeria monocytogenes prfA assay-reduction of ambiguous...

17. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type

    Blog Post

    Published

    Oct. 4, 2018, 12:44 p.m.

    ... selected and ranked based on the previously established sgRNA rules and  potential on- and off-target...

18. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type

    Blog Post

    Published

    Dec. 7, 2020, 2:53 p.m.

    ...genome engineering in bacteria, alongside more established tools such as Cas9-recombineering, CRISPRa/i,...

19. Summer SciComm Series: Modes of Communication

    Type

    Blog Post

    Published

    July 1, 2022, 1:15 p.m.

    ...answered the above questions, you can create your short list of options and then pick whichever is most accessible...

20. Plasmids 101: Colony PCR

    Type

    Blog Post

    Published

    May 12, 2016, 2:30 p.m.

    ...restriction enzyme digest; however colony PCR can accomplish the same thing in less time and for less money...