We narrowed to 619 results for: LIS;

...could make a model of Cas9 based on the structure published by Martin Jinek’s group. We had already made an...

...protein, we have used Western blots, dot blots, ELISAs, and enzyme assays to select both over- and under-expressing...

... stocks that are available to everyone. Flybase lists what stocks are available at Bloomington, but you...

...Tompkins, G. J., & McCawley, P. (1977). The establishment of two cell lines from the insectspodoptera ...

...range of topics. He embraces open science, self-publishing directly on his website (and often posting updates...

..., M., Farouni, R., Lagler, S., Kalamakis, G., Lischetti, U., Jabaudon, D., & Platt, R. J. (2023). Transcriptional...

... for you — and the mice. While not an exhaustive list, these can include what’s being injected, the type...

...gene of interest will direct the investigator to a list of all available Addgene plasmids he or she can ...

...handy guide from OpenWetWare for a more extensive listing of common E. coli strains. Increase the volume ...

...dimeric FP could ruin your day (and experiment) are listed below. Solutions to avoid these all too common ...

...negligible level. One major reason we chose to publish the concept and likely capabilities of RNA-guided...

...purification; the whole cloning process is so simplistic once you have your parts. That was the motivation...

...alignment tools available. Try these resources for lists of alternatives to BLAST: ExPASy - http://www.expasy.org...

...pH changes (e.g. pHluorin, pHTomato). Keep this list handy as you plan your next experiment or to hand...

... selected and ranked based on the previously established sgRNA rules and  potential on- and off-target...

...answered the above questions, you can create your short list of options and then pick whichever is most accessible...

...genome engineering in bacteria, alongside more established tools such as Cas9-recombineering, CRISPRa/i,...

...embed_player" overrideable=False, type='scriptV4', hide_playlist=True, viral_sharing=False, embed_button=False...

...adapted from the 1979 Patterson Protocol. When published, the Patterson Protocol was shown to purify DNA...

... toxic to dividing cells. The other antibiotics listed below affect continuous cellular processes (e.g...