We narrowed to 618 results for: LIS;

1. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

   Type

   Blog Post

   Published

   July 13, 2017, 2:30 p.m.

   ... stocks that are available to everyone. Flybase lists what stocks are available at Bloomington, but you...

2. Hot Plasmids and Viral Preps - May 2021

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   Blog Post

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   May 18, 2021, 1:15 p.m.

   ...synthetic human scFV library and characterized them by ELISA, homogeneous time resolved fluorescence, and suspension...

3. The AAVantages of AAV in CRISPR Screens

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   Blog Post

   Published

   Feb. 4, 2025, 2:15 p.m.

   ..., M., Farouni, R., Lagler, S., Kalamakis, G., Lischetti, U., Jabaudon, D., & Platt, R. J. (2023). Transcriptional...

4. Common Injection Routes in Mice

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   Blog Post

   Published

   Jan. 22, 2025, 9:42 p.m.

   ... for you — and the mice. While not an exhaustive list, these can include what’s being injected, the type...

5. Production of Virus in Insect Versus Mammalian Cells

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   Blog Post

   Published

   Nov. 5, 2024, 8:32 p.m.

   ...Tompkins, G. J., & McCawley, P. (1977). The establishment of two cell lines from the insectspodoptera ...

6. Magnetic Control of Proteins: More than a Dream

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   Blog Post

   Published

   Sept. 24, 2024, 1:15 p.m.

   ...range of topics. He embraces open science, self-publishing directly on his website (and often posting updates...

7. 3D Printing Meets CRISPR Cas9

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   Blog Post

   Published

   Oct. 17, 2017, 12:55 p.m.

   ...could make a model of Cas9 based on the structure published by Martin Jinek’s group. We had already made an...

8. Addgene's Cancer Collection Pages Connect the Research Community

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   Blog Post

   Published

   Sept. 6, 2016, 2:30 p.m.

   ...gene of interest will direct the investigator to a list of all available Addgene plasmids he or she can ...

9. Plasmids 101: Optimizing Plasmid Yields

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   Blog Post

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   May 26, 2016, 2:30 p.m.

   ...handy guide from OpenWetWare for a more extensive listing of common E. coli strains. Increase the volume ...

10. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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    Blog Post

    Published

    April 19, 2016, 2:30 p.m.

    ...dimeric FP could ruin your day (and experiment) are listed below. Solutions to avoid these all too common ...

11. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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    Blog Post

    Published

    May 22, 2015, 4:58 p.m.

    ...negligible level. One major reason we chose to publish the concept and likely capabilities of RNA-guided...

12. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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    Blog Post

    Published

    June 12, 2014, 3:30 p.m.

    ...purification; the whole cloning process is so simplistic once you have your parts. That was the motivation...

13. Tips for Using BLAST to Verify Plasmids

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    Blog Post

    Published

    May 29, 2014, 1:29 p.m.

    ...alignment tools available. Try these resources for lists of alternatives to BLAST: ExPASy - http://www.expasy.org...

14. Which Fluorescent Protein Should I Use?

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    Blog Post

    Published

    May 20, 2014, 2:06 p.m.

    ...pH changes (e.g. pHluorin, pHTomato). Keep this list handy as you plan your next experiment or to hand...

15. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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    Blog Post

    Published

    Oct. 4, 2018, 12:44 p.m.

    ... selected and ranked based on the previously established sgRNA rules and  potential on- and off-target...

16. Summer SciComm Series: Modes of Communication

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    Blog Post

    Published

    July 1, 2022, 1:15 p.m.

    ...answered the above questions, you can create your short list of options and then pick whichever is most accessible...

17. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    Blog Post

    Published

    Dec. 7, 2020, 2:53 p.m.

    ...genome engineering in bacteria, alongside more established tools such as Cas9-recombineering, CRISPRa/i,...

18. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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    Blog Post

    Published

    July 16, 2020, 1:15 p.m.

    ...embed_player" overrideable=False, type='scriptV4', hide_playlist=True, viral_sharing=False, embed_button=False...

19. DNA Purification Without a Kit

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    Blog Post

    Published

    Feb. 11, 2020, 2:15 p.m.

    ...adapted from the 1979 Patterson Protocol. When published, the Patterson Protocol was shown to purify DNA...

20. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type

    Blog Post

    Published

    Oct. 10, 2023, 1 p.m.

    ... toxic to dividing cells. The other antibiotics listed below affect continuous cellular processes (e.g...