We narrowed to 644 results for: Rest
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TypeBlog PostPublishedDec. 8, 2022, 2:15 p.m....multiplexable RNA knockdown. Cas13 has also stirred up interest for its potential as a viral therapeutic that ...
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Giving gRNAs a Facelift - Synthetic and Beyond
TypeBlog PostPublishedOct. 20, 2022, 1:15 p.m....These modifications result in conformationally restricted RNA which improves mismatch discrimination and... -
Addgene's Tips for Plasmid Quality Control
TypeBlog PostPublishedJan. 14, 2016, 3:30 p.m....functional mutations, antibiotic resistance, restriction sites used during cloning, and most importantly... -
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP
TypeBlog PostPublishedNov. 24, 2015, 3:30 p.m.... a lox-STOP-lox cassette upstream of a gene of interest blocks transcription when Cre is absent. When ... -
The Future of Research Symposium Boston 2015
TypeBlog PostPublishedOct. 20, 2015, 2:30 p.m....understand human disease. Outside the lab, he is interested in communicating science and in the intersection... -
Career Coaching for Scientists: Why and Where Do I Find One?
TypeBlog PostPublishedJan. 29, 2015, 1:25 p.m....Coach De La Professional but the website looks interesting and it is in English and Spanish. She is based... -
A Look at Addgene's QC Process
TypeBlog PostPublishedMay 6, 2025, 1:15 p.m.... sequencing all incoming plasmids. If you are interested in requesting one of these plasmids, you can ... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog PostPublishedOct. 8, 2024, 1:15 p.m....Genes allowing bacteria to survive toxins are the clearest example of a plasmid benefiting its host. For ... -
Antibodies 101: Stripping and Reprobing Western Blots
TypeBlog PostPublishedAug. 1, 2024, 1:15 p.m....blots are a great tool to identify a protein of interest in a complicated solution like cell lysate. But... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog PostPublishedJune 27, 2017, 1:03 p.m....and can be used to create fusions with a gene of interest. These plasmids will be most useful for constructing... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog PostPublishedMarch 8, 2017, 3:15 p.m....Gibson cloning, ligation-independent cloning, restriction enzyme, TOPO cloning, unknown, or other. ... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog PostPublishedJuly 23, 2020, 1:20 p.m....Addgene.org Find CRISPR Plasmids for Your Research Interested in light-inducible Cas9 activity? Check out these... -
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells
TypeBlog PostPublishedMay 7, 2020, 1:15 p.m....complex of TadA and dCas to the genomic DNA of interest Figure 1. When ABEs are in action they... -
Overcoming the Challenges of Lentiviral Production
TypeBlog PostPublishedAug. 7, 2018, 12:31 p.m....titer, and successfully transducing a cell line of interest a lot can go wrong. We hope that we have provided... -
A Guide to Designing a Scientific Poster: Content, Layout, and More
TypeBlog PostPublishedOct. 22, 2019, 12:20 p.m....You’ve done great research and made interesting discoveries. You’ve analyzed the data and generated beautiful... -
Lab Automation at Addgene
TypeBlog PostPublishedMarch 22, 2018, 1:28 p.m....I would recommend conferences like Lab Robotic Interest Group (LRIG). There is so much to see and so many... -
Hot Plasmids February 2024
TypeBlog PostPublishedFeb. 20, 2024, 2:45 p.m....protein, StayGold. Although it quickly saw wide interest, its dimeric nature can interfere with some applications... -
Viral Vectors 101: Chemogenetics
TypeBlog PostPublishedJuly 11, 2023, 1:15 p.m....development of therapies when expression must be restricted to specific circuits. Methods for targeting expression... -
Corralling and Cornering the Goldilocks Antibody
TypeBlog PostPublishedAug. 9, 2022, 1:15 p.m....and I am excited to be a part of it! If you are interested to get involved by submitting data, sending antibodies... -
6 Tips for Grant Writing
TypeBlog PostPublishedOct. 18, 2016, 2:30 p.m.... must be original (otherwise why would you be interested in it?) so make sure that comes across. Check...