We narrowed to 610 results for: SCR

1. PITChing MMEJ as an Alternative Route for Gene Editing

   Type

   Blog Post

   Published

   Feb. 23, 2016, 3:30 p.m.

   ...Building on the lab’s previous work, Sakuma et al. describe a detailed protocol for MMEJ-mediated knock-in...

2. SciComm: Taking Science to Elementary and Middle Schools

   Type

   Blog Post

   Published

   July 9, 2019, 12:52 p.m.

   ... itself in terms of what they do with their discretionary money. They also usually have a good event-planning...

3. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

   Type

   Blog Post

   Published

   Dec. 7, 2020, 2:53 p.m.

   ...Transform plasmid(s) into cells and plate overnight Screen colonies phenotypically or by PCR/sequencing ...

4. Plasmids 101: Choosing an Antibiotic Resistance Gene

   Type

   Blog Post

   Published

   Oct. 10, 2023, 1 p.m.

   ...Antibiotic Resistance Plasmids 101: Blue-White Screening Choosing Your Perfect Plasmid Backbone  ...

5. An Introduction to Adenovirus

   Type

   Blog Post

   Published

   Oct. 3, 2023, 1:15 p.m.

   ... (and therefore between early and late gene transcription) is demarcated by viral genome replication (...

6. CRISPR 101: Making a Knock-In Cell Line

   Type

   Blog Post

   Published

   Dec. 22, 2022, 2:15 p.m.

   ...reading up on validating genome edits to find screening strategies for your experiment. Also learn about...

7. A Guide to Starting Your Own Journal Club

   Type

   Blog Post

   Published

   Feb. 6, 2020, 3 p.m.

   ...served. I hope that with some of the information described above you feel empowered to start your own journal...

8. Tips for arabidopsis transformation

   Type

   Blog Post

   Published

   Oct. 25, 2018, 1:23 p.m.

   ...generate transgenic Arabidopsis, from studying transcriptional and translational dynamics of genes and proteins...

9. Simplify Cloning with in vivo Assembly

   Type

   Blog Post

   Published

   Oct. 18, 2018, 12:37 p.m.

   ... minimize false positive colonies. We routinely screen just two colonies for simple cloning procedures...

10. The Importance of a Fun Workplace: Company Culture at Addgene

    Type

    Blog Post

    Published

    Sept. 3, 2015, 2:30 p.m.

    ...side of things from taking too much of your time, scroll down for some tips on how to make a successful ...

11. Plasmids 101: Protein tags

    Type

    Blog Post

    Published

    Dec. 11, 2014, 4:26 p.m.

    ...examples. The tables list more common tags than are described in the post, but have been categorized to help...

12. Your Lentiviral Plasmid FAQs Answered

    Type

    Blog Post

    Published

    April 23, 2014, 1:08 p.m.

    ...our eBook: Plasmids 101 Learn about genome-wide screening with CRISPR and Lentivirus Read about the ClonTracr...

13. Negotiating Work and Life: How to Find the Joy

    Type

    Blog Post

    Published

    March 20, 2014, 3:04 p.m.

    ... on the Society for Laboratory Automation and Screening (SLAS) Electronic Laboratory Neighborhood.   Work...

14. Plasmids 101: Gateway Cloning

    Type

    Blog Post

    Published

    Jan. 12, 2017, 3:30 p.m.

    ...Gateway technology relies on the two reactions described below: The BP Reaction takes place between the...

15. Deciphering the Mysteries of Behavior with Viral Vectors

    Type

    Blog Post

    Published

    Sept. 17, 2019, 12:40 p.m.

    ...that were not (Hare et al., 2019). The research described here is only a small selection of the increasing...

16. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type

    Blog Post

    Published

    July 23, 2019, 12:46 p.m.

    ... the AAV9 capsid. This mutant library was then screened in vivo in C57BL/6J mice strains to identify variants...

17. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type

    Blog Post

    Published

    May 2, 2019, 12:41 p.m.

    ...genotype to phenotype studies New organismal systems described at this meeting were extraordinarily diverse. ...

18. CRISPR-mediated Plant Base Editors

    Type

    Blog Post

    Published

    Jan. 3, 2019, 1:35 p.m.

    ...into E. coli harboring the defective CamR gene. Screening for surviving colonies and subsequent rounds of...

19. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type

    Blog Post

    Published

    Sept. 16, 2020, 1 p.m.

    ...as potent and specific? The two AAV strategies described above showed successful target modification, indicating...

20. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type

    Blog Post

    Published

    July 30, 2024, 1:15 p.m.

    ...development. We’ve shared what we’ve learned, through manuscripts (Ersing et al., 2023; Haery et al., 2019; McDade...