We narrowed to 617 results for: SCR

...that perfect. One of the known limitations was described by Schüchner, et al., who published a paper showing...

...lab, research, and provide them with a CV or transcripts if requested Thank them, and eagerly await a ...

...better suited for genome-wide CRISPRi library screening experiments where maintaining library diversity...

... separately). Figure 5: Mechanism of ATLASCre. VAMP2 = synaptobrevin 2; At = ALFA-tag; BACE ...

...proteasome for degradation. Like several other tags described here, the dTAG uses a small molecule, a tag, and...

...Center are now available at Addgene along with a description of their experimental protocol. The three plasmids...

...concentration Low copy plasmids produce fewer transcripts of their antibiotic resistance genes and can ...

...Building on the lab’s previous work, Sakuma et al. describe a detailed protocol for MMEJ-mediated knock-in...

...served. I hope that with some of the information described above you feel empowered to start your own journal...

...Antibiotic Resistance Plasmids 101: Blue-White Screening Choosing Your Perfect Plasmid Backbone  ...

... (and therefore between early and late gene transcription) is demarcated by viral genome replication (...

...reading up on validating genome edits to find screening strategies for your experiment. Also learn about...

... itself in terms of what they do with their discretionary money. They also usually have a good event-planning...

...Transform plasmid(s) into cells and plate overnight Screen colonies phenotypically or by PCR/sequencing ...

...generate transgenic Arabidopsis, from studying transcriptional and translational dynamics of genes and proteins...

... minimize false positive colonies. We routinely screen just two colonies for simple cloning procedures...

...side of things from taking too much of your time, scroll down for some tips on how to make a successful ...

...examples. The tables list more common tags than are described in the post, but have been categorized to help...

...our eBook: Plasmids 101 Learn about genome-wide screening with CRISPR and Lentivirus Read about the ClonTracr...

... on the Society for Laboratory Automation and Screening (SLAS) Electronic Laboratory Neighborhood.   Work...