...non-homologous sequence or a series of single-nucleotide substitutions along a 1.5 kb tract, all without selectable...found that mating improved editing efficiency suggesting that sperm are more permissive to Cas9 editing...CRISPR plasmids page. Future perspectives It is interesting to note that recipients for the 2015 Breakthrough...

...tool for predicting CGBE editing outcomes and assisting sgRNA selection is available at http://www.sunlab.fun...CGBEs. What's next? Base transversion editing is still advancing rapidly. If it follows a similar path ... synthesized, templated sequence. And the story still isn’t over! Keep an eye out for our next post about...

... to test the selectivity of your antibody. But testing is not one size fits all. The approaches used will...found that it is often best to determine this by testing them. For intracellular targets, we usually test.... The specific approach will depend on the characteristics of the target and whether suitable cell types...

...-019-0193-0 Walton, R. T., Christie, K. A., Whittaker, M. N., & Kleinstiver, B. P. (2020). Unconstrained...variants to develop five new base editors with distinct PAM sequences, expanding the number of available...

...concepts. Each short video answers an Antibodies 101 question, like "what is an antibody?" or "what is an IgE...scicomm ♬ original sound - Addgene We'll be posting these animations regularly from now until May, ...

...decorating your lab for the upcoming wintertime festivities! Help us spread some cheer by snapping a photo...Christmas, Hanukkah, ugly sweaters, Flannel Fridays, Festivus— you name it, we want to see it.      All photos...

...upcoming wintertime festivities: Hanukkah, Christmas, ugly sweaters, Flannel Fridays, Festivus— you name it,...

...Experiment.” Whatever your antibody application question, the Antibody 101 eBook is a great place to start...reach out to [email protected] with any thoughts, questions, or feedback. Happy reading! ...

... continue to grow the ways we share science by hosting our first ever CRISPR Week on social media. #AddgeneCRISPRweek...like to see what you think so we’ll ask you some questions on Friday - share with us how you use CRISPR. ...

...ship to, first on a temporary and now on a more lasting basis.   Table 1: Popular Rabies and VSV-G Plasmids...license fee, will be required. If you have any questions on ordering plasmids, please feel free to email...

...genus members to the specified international institution. Requesters will need to provide a brief description...Bureau of Industry and Security. If you have any questions about this or other Addgene shipping and ordering...

...the details. Chapter 3 - Answering interesting biological questions using microscopy. Chpater 4 - Research...

...for your TikTok or Instagram story, we also have stickers to help express your emotions (while you’re on...when you use your favorite one. If you have any suggestions for future GIFs, feel free to let us know on ...

...Dear scientists using AAV, We hear your questions! How much virus should I inject? How long do I need... patterns, and more. If you have feedback or suggestions about the AAV Data Hub, we welcome you to share...

...antibody deposit (the Integrin collection) from the Institute for Protein Innovation. An Integrin Antibody ...Flow Cytometry Rob Hurt, whose expertise and suggestions were critical to the development and writing ...

... pPSU1 and pPSU2. When restriction digested with PstI or EcoRV, these plasmids generate 100 bp or 1 kb...prep kit increases the costs to $20-30 per ladder: still significantly cheaper than commercial ladders. Are...

...lab manager, research technician, or Principal Investigator. How-to guides for troubleshooting your experiments...involved. So join the conversation! Do you have a question about culturing iPS cells? Confused by which system...

...re following all safety regulations. That’s one sticky situation our customer service can’t get you out...our lab in Bayliss Building @SMS_UWA is getting festive 🎄🎁🎅🤶🎄 pic.twitter.com/rDaEjxTNZX— Heng Chooi...

... in Ghana. He’s done research at a number of institutions in Ghana and most recently got his PhD in cell...back to Ghana after his PhD and the need to de-mystify molecular biology. 25:51 - 30:56 : Further biology...

...students can sometimes be elusive or have a certain mystique that makes them difficult to approach for the ...to join? 11:25 - 11:50 : What is the number one question you think rotation students should ask senior ...