We narrowed to 612 results for: Sis
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TypeBlog PostPublishedJan. 19, 2023, 2:15 p.m....explore Myc’s role in cell proliferation and tumorigenesis. Using two different synthetic peptides corresponding...
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PhD Applications After COVID
TypeBlog PostPublishedJan. 5, 2023, 2:15 p.m....in-person events change seemingly daily (and are inconsistent between universities!) This guide tells you ... -
Chromoproteins: Colorful Proteins For Molecular Biology Experiments
TypeBlog PostPublishedFeb. 4, 2020, 2:15 p.m....., 2000). Chromoproteins also enhance corals’ resistance to mass bleaching, the loss of their symbiotic... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog PostPublishedApril 19, 2016, 2:30 p.m..... An imperfect solution is to restrict imaging analysis to cells expressing the lowest levels of the fusion... -
Anatomy of a Plasmid Page at Addgene
TypeBlog PostPublishedFeb. 4, 2016, 3:30 p.m....is shipped. Copy number (if known), antibiotic resistance, and optimal growth temperature are listed, as... -
CRISPR 101: Mammalian Expression Systems and Delivery Methods
TypeBlog PostPublishedSept. 24, 2015, 2:30 p.m....mismatch repair assay? Or is PCR followed by gel electrophoresis and/or next-generation sequencing more appropriate... -
Light Sheet Fluorescence Microscopy
TypeBlog PostPublishedJuly 16, 2015, 2:30 p.m....Jae Lee and Pantelis Tsoulfas. Jae K. Lee is an assistant Professor at the University of Miami Miller School... -
Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments
TypeBlog PostPublishedSept. 23, 2014, 3:05 p.m....deposited by David Root of the Broad Institute) has consistently been one of the repository’s most frequently... -
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection
TypeBlog PostPublishedDec. 12, 2013, 3:35 p.m....describing the development of “transcriptors,” transistor-like digital genetic switches that enable cellular... -
CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog PostPublishedSept. 6, 2018, 12:02 p.m....to directly deliver a ribonucleoprotein (RNP), consisting of the Cas9 protein in complex with a targeting... -
Transferable Skills Guide: Identifying Your Transferable Skills
TypeBlog PostPublishedJan. 11, 2018, 3:29 p.m....Organization Leadership Problem Solving Creativity Data analysis Listening Negotiation Career Planning Resources... -
Antibodies 101: Producing Recombinant Antibodies
TypeBlog PostPublishedAug. 13, 2024, 1:15 p.m....At its core, harvesting recombinant antibodies consists of separating antibodies from media, cells, and... -
Capturing a Moment with Marc Zimmer’s “The State of Science”
TypeBlog PostPublishedJuly 30, 2020, 1:15 p.m....five Addgene colleagues who read it with me, the consistent response from scientist and non-scientist alike... -
Course-Based Undergraduate Research Experiences (CUREs)
TypeBlog PostPublishedFeb. 9, 2023, 2:15 p.m....course recently used a combination of phylogenetic analysis, structural prediction, and bench work to identify... -
Summer SciComm Series: Modes of Communication
TypeBlog PostPublishedJuly 1, 2022, 1:15 p.m....elephants than it is to design one demonstrating mitosis. Consider also the amount and type of information... -
A Guide to Starting Your Own Journal Club
TypeBlog PostPublishedFeb. 6, 2020, 3 p.m....recurring journal clubs on Immunology and Microbial Pathogenesis that occurred once a week for several months... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog PostPublishedAug. 9, 2016, 2:30 p.m....which of these 13,000 regions are critical? One hypothesis: the enhancers! This leads us to a three-step... -
Plasmids 101: Colony PCR
TypeBlog PostPublishedMay 12, 2016, 2:30 p.m..... If you are going to complete your colony PCR analysis in the same day, you can save the leftover bacteria-water... -
The Importance of a Fun Workplace: Company Culture at Addgene
TypeBlog PostPublishedSept. 3, 2015, 2:30 p.m....made all the difference here at Addgene is the emphasis placed on team building throughout the years. ... -
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design
TypeBlog PostPublishedSept. 13, 2018, 1:55 p.m....safe-targeting guides vs. non-targeting guides on screen analysis and hit calling. 5,644 non-targeting guides and...