We narrowed to 608 results for: Sis;

1. CRISPR 101: Mammalian Expression Systems and Delivery Methods

   Type

   Blog Post

   Published

   Sept. 24, 2015, 2:30 p.m.

   ...mismatch repair assay? Or is PCR followed by gel electrophoresis and/or next-generation sequencing more appropriate...

2. Light Sheet Fluorescence Microscopy

   Type

   Blog Post

   Published

   July 16, 2015, 2:30 p.m.

   ...Jae Lee and Pantelis Tsoulfas. Jae K. Lee is an assistant Professor at the University of Miami Miller School...

3. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

   Type

   Blog Post

   Published

   Dec. 12, 2013, 3:35 p.m.

   ...describing the development of “transcriptors,” transistor-like digital genetic switches that enable cellular...

4. Summer SciComm Series: Modes of Communication

   Type

   Blog Post

   Published

   July 1, 2022, 1:15 p.m.

   ...elephants than it is to design one demonstrating mitosis.   Consider also the amount and type of information...

5. Antibodies 101: Producing Recombinant Antibodies

   Type

   Blog Post

   Published

   Aug. 13, 2024, 1:15 p.m.

   ...At its core, harvesting recombinant antibodies consists of separating antibodies from media, cells, and...

6. Capturing a Moment with Marc Zimmer’s “The State of Science”

   Type

   Blog Post

   Published

   July 30, 2020, 1:15 p.m.

   ...five Addgene colleagues who read it with me, the consistent response from scientist and non-scientist alike...

7. A Guide to Starting Your Own Journal Club

   Type

   Blog Post

   Published

   Feb. 6, 2020, 3 p.m.

   ...recurring journal clubs on Immunology and Microbial Pathogenesis that occurred once a week for several months...

8. Course-Based Undergraduate Research Experiences (CUREs)

   Type

   Blog Post

   Published

   Feb. 9, 2023, 2:15 p.m.

   ...course recently used a combination of phylogenetic analysis, structural prediction, and bench work to identify...

9. CRISPR 101: Ribonucleoprotein (RNP) Delivery

   Type

   Blog Post

   Published

   Sept. 6, 2018, 12:02 p.m.

   ...to directly deliver a ribonucleoprotein (RNP), consisting of the Cas9 protein in complex with a targeting...

10. Transferable Skills Guide: Identifying Your Transferable Skills

    Type

    Blog Post

    Published

    Jan. 11, 2018, 3:29 p.m.

    ...Organization Leadership Problem Solving Creativity Data analysis Listening Negotiation Career Planning Resources...

11. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type

    Blog Post

    Published

    Aug. 9, 2016, 2:30 p.m.

    ...which of these 13,000 regions are critical? One hypothesis: the enhancers! This leads us to a three-step...

12. Plasmids 101: Colony PCR

    Type

    Blog Post

    Published

    May 12, 2016, 2:30 p.m.

    .... If you are going to complete your colony PCR analysis in the same day, you can save the leftover bacteria-water...

13. The Importance of a Fun Workplace: Company Culture at Addgene

    Type

    Blog Post

    Published

    Sept. 3, 2015, 2:30 p.m.

    ...made all the difference here at Addgene is the emphasis placed on team building throughout the years. ...

14. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type

    Blog Post

    Published

    Sept. 23, 2014, 3:05 p.m.

    ...deposited by David Root of the Broad Institute) has consistently been one of the repository’s most frequently...

15. New CRISPR Tools: Cas7-11 and PASTE

    Type

    Blog Post

    Published

    Feb. 17, 2022, 2 p.m.

    ... Consider, for instance, a disease like cystic fibrosis, which can be caused by thousands of different...

16. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type

    Blog Post

    Published

    July 30, 2024, 1:15 p.m.

    ...Our viral vectors data is less amenable to such analysis, due to its inherent bias: Addgene uses a prioritization...

17. Hot Plasmids Spring 2024

    Type

    Blog Post

    Published

    May 23, 2024, 1:15 p.m.

    ... research; however, microglia are notoriously resistant to viral transduction. Through in vivo screening...

18. Tips for Writing a Good Cover Letter

    Type

    Blog Post

    Published

    May 21, 2024, 1:30 p.m.

    ...department] at [name of the university].During my thesis work on [phenomenon X], I read several of your ...

19. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type

    Blog Post

    Published

    Aug. 20, 2020, 2:30 p.m.

    ...JK, Been KW, Yoon S, Kim J-S (2016) Genome-wide analysis reveals specificities of Cpf1 endonucleases in...

20. Finding nucleic acids with SHERLOCK and DETECTR

    Type

    Blog Post

    Published

    April 16, 2020, 1 p.m.

    ...science.aas8836 Niewoehner O, Jinek M (2016) Structural basis for the endoribonuclease activity of the type III-A...