We narrowed to 613 results for: Sis;

1. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

   Type

   Blog Post

   Published

   June 25, 2024, 1:15 p.m.

   ...a prime editing guide RNA (pegRNA). The pegRNA consists of a target-complementary spacer, a scaffold sequence...

2. Pooled Library Amplifications

   Type

   Blog Post

   Published

   May 30, 2024, 1:30 p.m.

   ...Screening pCMV-Rep78/68 Scanning Saturation Mutagenesis (SSM) Pooled Library 80,962 1866 bp, plus...

3. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

   Type

   Blog Post

   Published

   Feb. 4, 2020, 2:15 p.m.

   ...., 2000). Chromoproteins also enhance corals’ resistance to mass bleaching, the loss of their symbiotic...

4. Transferable Skills Guide: Managing a Team

   Type

   Blog Post

   Published

   Jan. 30, 2018, 2:42 p.m.

   ... happening even though I met with X on a weekly basis! The silence and maybe even the slight awkwardness...

5. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

   Type

   Blog Post

   Published

   April 19, 2016, 2:30 p.m.

   .... An imperfect solution is to restrict imaging analysis to cells expressing the lowest levels of the fusion...

6. Anatomy of a Plasmid Page at Addgene

   Type

   Blog Post

   Published

   Feb. 4, 2016, 3:30 p.m.

   ...is shipped. Copy number (if known), antibiotic resistance, and optimal growth temperature are listed, as...

7. CRISPR 101: Mammalian Expression Systems and Delivery Methods

   Type

   Blog Post

   Published

   Sept. 24, 2015, 2:30 p.m.

   ...mismatch repair assay? Or is PCR followed by gel electrophoresis and/or next-generation sequencing more appropriate...

8. Light Sheet Fluorescence Microscopy

   Type

   Blog Post

   Published

   July 16, 2015, 2:30 p.m.

   ...Jae Lee and Pantelis Tsoulfas. Jae K. Lee is an assistant Professor at the University of Miami Miller School...

9. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

   Type

   Blog Post

   Published

   Sept. 23, 2014, 3:05 p.m.

   ...deposited by David Root of the Broad Institute) has consistently been one of the repository’s most frequently...

10. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type

    Blog Post

    Published

    Dec. 12, 2013, 3:35 p.m.

    ...describing the development of “transcriptors,” transistor-like digital genetic switches that enable cellular...

11. Course-Based Undergraduate Research Experiences (CUREs)

    Type

    Blog Post

    Published

    Feb. 9, 2023, 2:15 p.m.

    ...course recently used a combination of phylogenetic analysis, structural prediction, and bench work to identify...

12. Antibodies 101: Producing Recombinant Antibodies

    Type

    Blog Post

    Published

    Aug. 13, 2024, 1:15 p.m.

    ...At its core, harvesting recombinant antibodies consists of separating antibodies from media, cells, and...

13. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type

    Blog Post

    Published

    July 30, 2020, 1:15 p.m.

    ...five Addgene colleagues who read it with me, the consistent response from scientist and non-scientist alike...

14. A Guide to Starting Your Own Journal Club

    Type

    Blog Post

    Published

    Feb. 6, 2020, 3 p.m.

    ...recurring journal clubs on Immunology and Microbial Pathogenesis that occurred once a week for several months...

15. Transferable Skills Guide: Identifying Your Transferable Skills

    Type

    Blog Post

    Published

    Jan. 11, 2018, 3:29 p.m.

    ...Organization Leadership Problem Solving Creativity Data analysis Listening Negotiation Career Planning Resources...

16. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type

    Blog Post

    Published

    Aug. 9, 2016, 2:30 p.m.

    ...which of these 13,000 regions are critical? One hypothesis: the enhancers! This leads us to a three-step...

17. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type

    Blog Post

    Published

    Sept. 6, 2018, 12:02 p.m.

    ...to directly deliver a ribonucleoprotein (RNP), consisting of the Cas9 protein in complex with a targeting...

18. Summer SciComm Series: Modes of Communication

    Type

    Blog Post

    Published

    July 1, 2022, 1:15 p.m.

    ...elephants than it is to design one demonstrating mitosis.   Consider also the amount and type of information...

19. Plasmids 101: Colony PCR

    Type

    Blog Post

    Published

    May 12, 2016, 2:30 p.m.

    .... If you are going to complete your colony PCR analysis in the same day, you can save the leftover bacteria-water...

20. The Importance of a Fun Workplace: Company Culture at Addgene

    Type

    Blog Post

    Published

    Sept. 3, 2015, 2:30 p.m.

    ...made all the difference here at Addgene is the emphasis placed on team building throughout the years. ...