We narrowed to 630 results for: Son
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TypeBlog PostPublishedMarch 11, 2025, 1:15 p.m....plasmids here! Vu, T. V., Nguyen, N. T., Kim, J., Song, Y. J., Nguyen, T. H., & Kim, J.-Y. (2024). Optimized...
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Polymerase Chain Reaction Overview and Applications
TypeBlog PostPublishedAug. 10, 2021, 1:15 p.m....droplets is determined and then analyzed using Poisson statistics to determine the concentration of the... -
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies
TypeBlog PostPublishedJuly 13, 2017, 2:30 p.m....Y or fourth chromosomes, you are a very unlucky person. Animals are diploid except for their germ cells... -
Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR
TypeBlog PostPublishedJuly 2, 2020, 12:40 p.m....Li M, Zhou F, Li K, Cao H, Ni M, Liu Y, Gu Z, Dickerson KE, Xie S, Hon GC, Xuan Z, Zhang MQ, Shao Z, Xu... -
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments
TypeBlog PostPublishedFeb. 7, 2019, 2:16 p.m....Chan Zuckerberg Biohub Manuel Leonetti Ryan Leenay Jason Li Max Haeussler Luca Pinello Kendell Clement Andy... -
Academic vs. Industry Postdocs
TypeBlog PostPublishedNov. 13, 2018, 1:22 p.m....is not always the case. There are “difficult personalities” in every work setting and no manager will ever... -
Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine
TypeBlog PostPublishedOct. 30, 2018, 1:04 p.m....forward the next year when both the Yamanaka and Thomson labs showed the successful generation of human ... -
Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity
TypeBlog PostPublishedOct. 11, 2018, 12:30 p.m.... efforts for over 50 years, prefers ligation of Watson-Crick base pair substrates, it demonstrates significant... -
Cloning Mammalian Cells with the Agarose Method
TypeBlog PostPublishedSept. 7, 2017, 12:17 p.m....cloning. Taking a page from bacterial cloning, we reasoned that we should be able to keep cells moist and... -
Addgene's Cancer Collection Pages Connect the Research Community
TypeBlog PostPublishedSept. 6, 2016, 2:30 p.m....Cancer – a term familiar to almost every person regardless of profession, race, or gender. Almost no ... -
Plasmids 101: Optimizing Plasmid Yields
TypeBlog PostPublishedMay 26, 2016, 2:30 p.m....of the empty plasmid backbone or a researcher’s personal observations. If you are experiencing low yields... -
Genome-wide Screening Using CRISPR
TypeBlog PostPublishedAug. 18, 2015, 2:30 p.m....Resources Page. References McDade JR, Waxmonsky NC, Swanson LE, Fan M (2016) Practical Considerations for Using... -
Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards
TypeBlog PostPublishedMay 22, 2015, 4:58 p.m....accidental escape to a negligible level. One major reason we chose to publish the concept and likely capabilities... -
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More
TypeBlog PostPublishedJune 12, 2014, 3:30 p.m....talking to people and representing yourself as a real person, so that students see academics as well-rounded... -
Tips for Using BLAST to Verify Plasmids
TypeBlog PostPublishedMay 29, 2014, 1:29 p.m.... Jason Niehaus... -
Which Fluorescent Protein Should I Use?
TypeBlog PostPublishedMay 20, 2014, 2:06 p.m....effect called FRET (fluorescence [or Förster] resonance energy transfer). FRET occurs when energy transfer... -
Great Results Start with Great Standard Curves
TypeBlog PostPublishedAug. 15, 2024, 1:15 p.m.... ELISAs and When to Use Them A Control for All Seasons More resources on addgene.org Addgene's Antibody... -
Getting to Know Expansion Microscopy
TypeBlog PostPublishedJune 20, 2023, 1:15 p.m..../10.1126/science.1260088 Damstra HG, Mohar B, Eddison M, et al (2022) Visualizing cellular and tissue... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog PostPublishedAug. 27, 2020, 1:15 p.m....Medical Center. You’ve probably heard of Forster Resonance Energy Transfer (FRET). Through the non-radiative... -
CRISPR 101: RNA Editing with Cas13
TypeBlog PostPublishedJuly 31, 2020, 12:30 p.m....off-target editing. In one direct transcriptome-wide comparison, REPAIRv2 reduced off-target sites from the 18,385...