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We narrowed to 919 results for: addgene

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Showing: 561 - 580 of 919 results

  1. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...Resources on Addgene.org CRISPR software and resources Addgene CRISPR Guide Addgene’s CRISPR 101 eBook... eBook More resources on the Addgene blog CRISPR 101: Which Cas9 do I choose for my CRISPR experiment...

  2. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ... of a tagged protein. Resources on Addgene.org: Browse Addgene’s Antibody Plasmid Collection Find antibodies...10.3390/ijms21176324  Additional resources on the Addgene blog: Read about frankenbodies, an anti-HA scFv-based...neuroscience research from the Trimmer lab Check out Addgene's latest project to develop an open antibody resource...

  4. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ...Luke Makana Hanley is a Research Team Lab Tech at Addgene working on methods to improve AAV production.   ...PMCID: PMC5538794. Additional Resources on the Addgene Blog AAV Titers: Where do they come from and what...Expression in Mammals Additional Resources on Addgene.org Find Ready-to-Use AAV Preps Find Lab Protocols...

  5. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...obtaining a plasmid with the gene of interest (search Addgene plasmids by gene here), designing primers with ... profession.       Additional Resources on the Addgene Blog Learn More about Gateway Cloning Gibson Assembly...Plasmids by Colony PCR Addtional Resources on Addgene.org Browse Our CRISPR Resources Find Validated gRNAs...

  7. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...CRISPR-Sirius a try. Find CRISPR-Sirius plasmids at Addgene. References Ma, Hanhui et al. “Multicolor CRISPR...PubMed PMID: 30377374. Additional resources on the Addgene blog Download our CRISPR eBook Learn about fluorescent...tools Read all CRISPR blog posts Resources on Addgene.org Browse our CRISPR plasmids for visualization...

  8. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ...viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...PMCID: PMC5374486. Additional resources on the Addgene blog Read all of our viral vectors blog posts ...

  9. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...detailed manual and online design examples of using Addgene plasmids hCtRNA_FT (#186715) and hCtRNA_VT (#186716...References and Resources More resources on the Addgene blog CRISPR 101: Multiplex expression of gRNAs ...

  10. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ... Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ...  References and Resources More resources on Addgene’s blog Antibodies 101: Immunoprecipitation  Antibodies...

  11. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ... the Addgene Blog Get Started with AAV Learn more about Lentivirus Find out More about Addgene's Viral...Viral Service Resources on Addgene.org Visit Our AAV Guide Page Find Ready-to-use Virus for Your Research...

  12. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ... not yet available, just ask an Addgene scientist at help@addgene.org! References and Resources  References...10.1038/nprot.2017.016. Additional Resources on the Addgene Blog Learn More about Lentivirus Figure out How...Lentiviral Transduction Additional Resources on Addgene.org Plasmid Pooled Libraries Pooled library amplification...

  14. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...mutagenesis experiments? Find EvolvR plasmids at Addgene! References Gaj, Thomas, and Pablo Perez-Pinera... 36.9 (2018): 819. Additional resources on the Addgene blog Learn about other CRISPR methods for bacterial...Mutagenesis to mutate your plasmid Resources on Addgene.org Browse our CRISPR guide pages Find bacterial...

  15. Meet Aavery, Blugene's New Virus Friend!

    Type
    Blog Post
    Published
    Feb. 13, 2020, 2:34 p.m.
    ...you to meet..... *drumroll* Aavery! Welcome to Addgene, Aavery!       Original post: In December, we...

  16. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...PMCID: PMC4121734. Additional Resources on the Addgene Blog Fluorescent microscopy techniques - Which...protein-protein interactions Deposit plasmids with Addgene! ... tagging strategies Additional Resources on Addgene.org Fluorescent protein plasmids Use FRET to study...

  17. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ...mVenus as a reference protein is available from Addgene. Another way to dodge cellular variation observed...based biosensors.     Additional Resources on the Addgene Blog Which Fluorescent Protein Should I Use? Choosing... Protein Biosensors Additional Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find...

  18. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...PubMed Central PMCID: PMC6104080. Resources on the Addgene Blog Find microbiology blog posts Read about synthetic...Deposit microbiology plasmids with Addgene Find synthetic biology plasmids ...of our Plasmids 101 blog posts Resources on Addgene.org Check out our microbiology resource page Deposit...

  19. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ...find the plasmids for FlipGFP and FlipCherry at Addgene. References Cabantous, Stéphanie, et al. "A new...PubMed PMID: 30821975. Additional resources on the Addgene blog Read our fluorescent proteins blog posts ...Read our plasmids 101 blog series Resources on Addgene.org Browse the fluorescent protein collection Find...
Showing: 561 - 580 of 919 results