We narrowed to 664 results for: cell

1. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

   Type

   Blog Post

   Published

   Sept. 14, 2021, 1:15 p.m.

   ...efficient genome editing in Escherichia coli. Microb Cell Fact 19. https://doi.org/10.1186/s12934-020-01321...

2. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

   Type

   Blog Post

   Published

   April 23, 2020, 1:15 p.m.

   ... small amount of spurious DNA from the packaging cell line, helper and packaging plasmids. We decided ...

3. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

   Type

   Blog Post

   Published

   Nov. 20, 2018, 8:17 p.m.

   ...example, several journals like eLife, Nature, and Cell use  Research Resource identifiers (RRIDs) which...

4. Identifying Sequence Elements with SnapGene's Feature Database

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   Blog Post

   Published

   Dec. 21, 2017, 2:06 p.m.

   ..., and also a Professor of Molecular Genetics and Cell Biology at the University of Chicago. He was an ...

5. Plasmids 101: TOPO Cloning

   Type

   Blog Post

   Published

   Oct. 27, 2016, 2:30 p.m.

   ... Transform TOPO Cloning Reaction into Competent Cells: You can use your standard lab protocol for this...

6. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

   Type

   Blog Post

   Published

   July 7, 2015, 3:36 p.m.

   ...systems may prove effective in other species or in cell culture. In addition to CRISPR/Cas9 applications...

7. History of CRISPR Cas - A tale of survival and evolution

   Type

   Blog Post

   Published

   Nov. 25, 2013, 10:12 p.m.

   ...devise new strategies to reproduce within a host cell. A few virus particles have learned to circumvent...

8. Addgene Depositors Get More Citations

   Type

   Blog Post

   Published

   April 20, 2017, 2:30 p.m.

   ... most relevant to plasmids: Genetics & Heredity, Cell Biology, Biotechnology & Applied Microbiology, Biochemistry...

9. From Lab Bench to Leadership: Transitioning into Management as a PhD

   Type

   Blog Post

   Published

   Jan. 21, 2025, 2:15 p.m.

   ...team potential. I often thought that performing a cell culture experiment myself would take less time than...Another team member was highly self-motivated and excelled when given the freedom to explore projects autonomously...

10. Learning Technical Skills at a Lab Central Workshop

    Type

    Blog Post

    Published

    Feb. 20, 2018, 2:30 p.m.

    ...day was Julie from Novartis who was working with cell culture and who was extremely helpful since she ...

11. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type

    Blog Post

    Published

    May 19, 2020, 1:15 p.m.

    ...modification may not be easily expressed in non-mammalian cells. Yeast expression plasmids The yeast expression ...

12. Summer SciComm Series: A PhD in Science Communication

    Type

    Blog Post

    Published

    Aug. 23, 2022, 1:15 p.m.

    ...spend the afternoon lovingly tending to the stem cells I was using in an experiment, then spend the evening...

13. Dos & Don'ts When Publishing a Scientific Manuscript

    Type

    Blog Post

    Published

    May 22, 2014, 4:02 p.m.

    ...manuscripts submitted for publication. Holds a PhD in Cell Biology and a Masters degree in Infection and Immunity...

14. A Needle in a Base-Stack: Cas9 Structural Biology

    Type

    Blog Post

    Published

    June 4, 2024, 1:15 p.m.

    ...Guide RNA and Target DNA. Cell, 156(5), 935–949. https://doi.org/10.1016/j.cell.2014.02.001 Osuka, S., Isomura...efficiency and are important for robust activity in cells (Jiang & Doudna, 2017). Within an interior cavity...it, so many researchers think the enzyme needs cellular recycling factors to release its handiwork (Sternberg...

15. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type

    Blog Post

    Published

    June 20, 2017, 2:30 p.m.

    ...measure the pH of our samples, we used a solution of cell culture medium, which is buffered and contains the...

16. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type

    Blog Post

    Published

    Nov. 5, 2019, 1:28 p.m.

    ...CRISPR-Cas12b for mammalian genome engineering." Cell discovery 4.1 (2018): 63. PubMed PMID: 30510770....

17. Developing a cold tolerant E. coli using specialized chaperones

    Type

    Blog Post

    Published

    Jan. 28, 2020, 2:35 p.m.

    ...industrial or research purposes requires stable host cells (the simplest example would be a transformed mesophilic...

18. Summer SciComm: Preprints

    Type

    Blog Post

    Published

    Aug. 16, 2022, 1:15 p.m.

    ...Lacy was also employed by the American Society for Cell Biology in a position working with preprints in ...

19. Antibodies 101: Selecting the Right Antibody

    Type

    Blog Post

    Published

    March 15, 2022, 1:15 p.m.

    ...expression in knock-out or knock-down down tissues and cell lines; orthogonal strategies that compare antibody... project aimed at understanding how a critical cellular pathway is regulated. You’ve read all the background...

20. Early Career Researcher Toolbox: Social Media for Scientists

    Type

    Blog Post

    Published

    Dec. 11, 2018, 12:46 p.m.

    ...Science Communication” at the American Society for Cell Biology (ASCB) 2018 Conference. I don’t exactly ...