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Showing: 561 - 580 of 793 results

  1. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...labeling. Here, Liu et al. engineer a chimeric single chain variable fragment (scFv), grafting the binding region...fusion proteins, such as Cas9 and Cas12a, creates challenges for in vivo delivery and cell engineering applications...

  2. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...molecular biology and genetics. It can be used to characterize the function of certain regions in a promoter...’ve conducted SDM using some of these methods, chances are you’d like to find a better one. Trehan et ...

  3. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...MTA system. We also started our blog and YouTube channel in 2013, which continue to grow today. “I generally...Neuroscience 2023 conference. In 2020, the world changed in response to the COVID-19 pandemic, and Addgene...

  4. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    .... Created with BioRender.com. In later characterization studies, the edited embryos proceeded to the...heterozygous mutations, it will clearly be much more challenging for homozygous mutations. Moreover, introducing...

  5. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...fluorescent measurements from red, blue, and green channels overlaid on a bright-field image. Image from Ma...intrachromosomal loci, a major benchmark in characterizing the 4D nucleome. CRISPRainbow: Scaling up to...

  6. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ... which target and cleave RNA through the same mechanism as traditional spCas9. If you are looking to modify...it perfect for targeting mRNA and useful as a mechanism of transient knockdown of gene expression. This...

  7. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...common method. These proteins bind the IgG heavy chain. Running the serum over a column of resin crosslinked...low abundance or whose conformational state may change. If your protein might become slightly denatured...

  8. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...something that broke down RNases before RNases had a chance to break down RNA upon cell lysis.  In the late...until homogenization and cell lysis. This can be challenging depending on your laboratory situation or tissue...

  9. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...recently identified Cas12f systems from uncultivated archaea are much smaller and would be ideal for such AAV...system (CasMINI) engineered from the uncultivated archaea Cas12f (Un1Cas12f1, or Cas14a1) system. The CasMINI...

  11. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ..., and is long enough that there is virtually no chance of it occurring randomly. Therefore, inserting ...recombination event in a single system. The Saccharomyces cerevisiae FLP-FRT recombination system is another...

  12. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...edited and the sequence of the edit, so its design changes for every new prime editing application. And optimizing... L., Schmidt, R., Solley, S. C., Gilbert, L. A., Chan, M. M., Bauer, D. E., Marson, A., Parsons, L. R....

  13. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...patient mutation or need to make a precise amino acid change, there is very little flexibility in the location...nucleotides are represented equally, that’s only a 1/16 chance of having a PAM at a given site. While it is possible...

  14. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...was limited (likely due to its long, negatively charged sgRNA), RNP delivery was much better with Cas12...and screening that led to identification and characterization of AAV.CAP-Mac. Image reproduced from Chuapoco...

  15. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...polymorphisms (SNPs) in the TTR gene and is molecularly characterized by misfolding of the TTR protein, which contributes...technology is rapidly evolving, and many of the challenges with therapies involve patient safety, effectiveness...

  16. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    Published
    June 14, 2018, 11:44 a.m.
    ...not integrate into the host’s genome. The main challenge with using AdV is that it triggers a strong immune...confluent and the media will turn yellow. Do NOT change the media (add 2-3 mL of fresh media once a week...

  18. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ...spirit include: Start using electronic multichannel and single channel pipettes. These can speed up any project...

  19. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...broadly reactive secondary antibody to increase the chance of primary/secondary antibody binding and therefore..., P. A., Lewis, G. P., & Fisher, S. K. (1993). Chapter 15 Postembedding Immunocytochemical Techniques ...

  20. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...sequencing of neurons from each class to fully characterize the transcriptome. The first data release for...C.elegans neural network, and Sibernetic, a fluid mechanics simulator that can be used to simulate muscle ...
Showing: 561 - 580 of 793 results