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Showing: 561 - 580 of 787 results

  1. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ...overcomes this limitation and allows for the flexible design and small scale production of antibodies. Problems...Their His tags allow for further purification, if desired, but it's not required for their use. Each RANbody...

  3. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ...ensure scientists everywhere can access plasmids despite the evolving export/import rules. Our shipping ...most popular website in the world. I know, right? Upgrades to the internal LIMS pages and excellent work ...

  4. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...business practices, like accepting e-signatures and designing a website users could easily navigate. This meant...Read the Addgene guide: https://www.addgene.org/guides/crispr/” — SirHamhands, responding to a request...

  5. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ...and Accelerate Research Discoveries) Challenge provides grants for rare disease research. This year is...mutations in one of these parts, the GNB1 gene, which codes for G𝛃1 subunits of G protein complexes, lead to...

  6. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...in one direction. Cas3 is thus not helpful for designer genome edits, but it is effective at wiping out...David, Chen, S., Janice, et. al. Programmed DNA destruction by miniature CRISPR-Cas14 enzymes. Science, 362...

  7. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ... other sources. Mode of communication Different modes of communication take up different amounts of mental...Maybe I needed to employ a visual aid instead of describing something verbally. Sometimes a group was just...

  9. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ...showing the potential for broad use. This kit is designed for use with acceptor vectors from the MoClo Toolkit...tracing Article contributed by Erin Sanders CreLite, designed by the Eisenhoffer lab, is a new tool that increases...

  10. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...using a resistance marker; however, it is often desirable to remove the marker after the initial selection... Combining this with some of the loxP methods described above, a genetic modification can be restricted...

  13. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ...reporter gene, which replaces the targeted gene and provides a way to track successfully engineered cells. ...genotype each animal to identify the ones with the desired genotype. Inducible and conditional systems Conditional...

  14. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ...method seems simple, I found it hard to remember to describe all four parts of a STAR answer once the interview... because sometimes companies have relaxed dress codes for interviews. You can also ask HR for clarification...

  15. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...perform differently over time), compatible with the desired applications (such as immunohistochemistry (IHC...antibodies, we have developed a criterion that includes accuracy, strength, and reproducibility. We test...

  16. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ... Guardian, BBC Breakfast or the Londonist. BBC provides a peak into their day-to-day business with popular...Co-Ordinating Centre for Public Engagement (NCCPE) provides on-site courses in public engagement for institutions...

  17. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...virus will not only ensure that your infection is designed in the best possible way, but it may also save...the transgene itself. Many researchers prefer to design primers that target the common features of viral...

  18. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...plasmids, especially those of the CRISPR variety (see slides from my talk below). Largely organized by Dmitry...CRISPR regions of the prokaryotic genome. Then he desribed how P. furiosus has systems to defend against ...

  19. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ... of the DNA with a high frequency of C and G nucleotides). The conversion of cytosine to 5-methylcytosine..., which binds selectively to lysine-methylated peptides. This construct is in the middle of a FRET pair...

  20. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...success You’ve put a great deal of thought into designing your poster – now it’s time to practice your delivery...stepping into a sea of posters – where do you begin? Design a game plan Prior to attending the conference, ...
Showing: 561 - 580 of 787 results