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TypeBlog PostPublishedApril 29, 2025, 1:30 p.m....minBG-jRGECO1a-WPRE3-BGHpA PHPeB Biosensor The Allen Institute for Brain Science New viral tool AiP14360:...minBG-jGCaMP8s-WPRE3-BGHpA PHPeB Biosensor The Allen Institute for Brain Science New viral tool AiP14361: ...minBG-jGCaMP8s-WPRE3-BGHpA PHPeB Biosensor The Allen Institute for Brain Science New viral tool pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE...
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Visualizing Your Plasmid Request Data
TypeBlog PostPublishedApril 11, 2023, 4 p.m....aggregation of this information can provide valuable insight to the most requested plasmid, the number of requested...their CSV file from Addgene by following these instructions, and then uploading the CSV with request data...to the dashboard (Fig. 2). Figure 2: Insert showing the upload function for the Addgene Requests... -
Addgene Stands with the Scientific Community
TypeBlog PostPublishedApril 23, 2025, 1:30 p.m....Here to Help Despite these challenges, Addgene remains steadfast in our mission to accelerate research...Addgene is uniquely positioned to help research institutions maximize their increasingly limited resources...investment isn't lost. Universities and research institutions can turn to Addgene as a trusted partner for... -
500,000 Plasmids Shipped and Counting!
TypeBlog PostPublishedMarch 26, 2015, 3:24 p.m....plasmid shipping look like at Addgene? Here's an inside peak into the daily workings in Addgene's lab. ...plasmids to more than 90,000 scientists at 5,200 institutions in 82 different countries. We continue to ship...plasmids. The partnerships and collaborations with institutions and tech transfer offices that facilitate MTAs... -
Fueled by Coffee at #SfN14
TypeBlog PostPublishedNov. 18, 2014, 7:15 p.m....Feng Zhang (of the Broad Institute of MIT and Harvard and the McGovern Institute for Brain Research) – and... the neuroscience community pulls off such an inspiring, thought-provoking, collaborative event – all ... -
Download Science-Themed Zoom Backgrounds from Addgene
TypeBlog PostPublishedApril 22, 2021, 1:15 p.m.... If you’re predominantly a plasmid user, instead, don’t fret; we have backgrounds for you as well... might want to check out Addgene’s Fluorescent Proteins blog topic to learn more about FRET and other ... Aliyah Weinstein... -
Keeping Up With CRISPR/Cas9
TypeBlog PostPublishedMarch 11, 2014, 5:55 p.m.... tool. A week later, researchers at the Broad Institute and MIT published another report showing Cas9'...target." As further proof of just how big and mainstream the news on CRISPR has gotten, consider these...30. (Pubmed) Programmable Removal of Bacterial Strains by Use of Genome-Targeting CRISPR-Cas Systems. ... -
Download Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies
TypeBlog PostPublishedFeb. 26, 2019, 1:40 p.m....position and my team at Addgene was created.”- Eric Perkins, Director of Science Product Management “I knew...mantra and never forgot it. Usually when I see inspiring artists who are thinking about getting into the... Find job listings at Addgene Get more career insight from Addgenies ... -
Addgene's Top Blog Posts from 2020
TypeBlog PostPublishedDec. 29, 2020, 2:15 p.m....the methods in this blog post. 8. Chromoproteins: Colorful Proteins For Molecular Biology Experiments ...Experiments by Jennifer Tsang Chromoproteins are a subset of the fluorescent protein family and they generate color... -
The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years
TypeBlog PostPublishedJan. 8, 2014, 3:10 p.m....individual plasmids to 5,000 different research institutions. This has given us a unique window into technology... life sciences. In this post, we'll give you an inside look at the Top 10 plasmid technologies distributed...journey since starting Addgene. Thank you for inspiring us with your breakthrough research and your commitment... -
Top Requested AAV of 2017: pmSyn1-EBFP-CRE
TypeBlog PostPublishedJan. 17, 2018, 2:57 p.m.... you already have a transgenic mouse line that contains a Cre-dependent mCherry cassette under the control...of these options would be very time consuming. Instead, a quick way to get the results you need would ...complicated ways to use this particular AAV. For instance, you might use it as part of a flex switch you... -
Stepping into a Virtual Internship: My Experience at Addgene
TypeBlog PostPublishedAug. 13, 2020, 1:21 p.m.... I was able to express my visions for what my Instagram posts would look like. While working remotely ...continuing Addgene’s Meet an Addgenie Series on Instagram. I interviewed fellow Addgenies about their role... Some of recent Meet an Addgenie posts on Instagram! From this project, I observed what it was ... -
Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot
TypeBlog PostPublishedDec. 5, 2019, 1:49 p.m....the page looked clean, professional, and modern. Instead of trying to place all of the content above the... regularly be paired with a variety of science-inspired friends. As you can see on the homepage, Blugene... you can find what you need and feel happy and inspired to tackle your next science challenge. If you ... -
pSiM24: Simplifying Plant Genetic Engineering
TypeBlog PostPublishedSept. 29, 2015, 2:30 p.m....plant species. Thus, binary vectors contain two origins of replication, one each for E. coli and Agrobacterium...promoter has been modified with duplicated enhancer domains and characterized in studies of tobacco, Arabidopsis...regions permit multiple regulatory elements to be inserted, further customizing gene expression. Sahoo et... -
Future of Research Conference - Remarkable Opening Session
TypeBlog PostPublishedOct. 7, 2014, 3 p.m....audience to use their useful energy and to choose institutions with allies engaged in training excellence to...experience and encouragement. Dr. Sibby Anderson-Thompkins, Director of the Office of Postdoctoral Affairs... training programs (like at the Keck Graduate Institute), funding for training (such as the NIH BEST award... -
Some Like it Hot: Thermostable GeoCas9
TypeBlog PostPublishedSept. 14, 2017, 12:40 p.m....bacteria. Until recently the only available Cas9 proteins were isolated from mesophilic (medium heat loving... as Streptococcus pyogene’s SpCas9. These Cas9 proteins don’t work well at high temperatures, so to use...when incubated in diluted human plasma. GeoCas9 remains active after being incubated in up to 30% human... -
Embracing Serendipity: A Crucial Element in the PhD Journey
TypeBlog PostPublishedApril 25, 2024, 1:15 p.m....knowledge base extensively, you open doors to fresh insights and broaden your understanding, ultimately enhancing...and connections that emerge during moments of inspiration. Additionally, journaling in the morning has ..., whether within academia or industry. Albert Einstein once said: “There are only two ways to live your... -
Early Career Researcher Toolbox: Free Online Molecular Biology Tools
TypeBlog PostPublishedSept. 12, 2023, 1:15 p.m....calculators These links can help you calculate how much insert and backbone you need for different types of cloning...method. You can select from five different insert:backbone ratios. In-Fusion cloning molar ratio calculators...IHC. FPbase: A data repository for fluorescent proteins and their properties. Expasy: Bioinformatics ... -
Cellosaurus: A Cell Line Information Database
TypeBlog PostPublishedFeb. 21, 2023, 2:15 p.m....were excited when Amos Bairoch from the Swiss Institute of Bioinformatics reached out to us to tell us...surprising, and important, findings being shared. For instance, recently Cellosaurus received a report that HT...out the Cellosaurus FAQ! References Lorsch JR, Collins FS, Lippincott-Schwartz J. Cell biology. Fixing... -
Chemogenetics vs. Optogenetics: Which Method Should I Choose?
TypeBlog PostPublishedJune 11, 2020, 1:34 p.m....activation to behaviors. Scientists continue to engineer opsins to operate at increasingly faster kinetics. The...by working with organisms with light accessible brains, such as zebrafish larvae. Mouse studies, however...permanent intracranial implant to be surgically installed to allow for light delivery. Chemogenetics poses...