We narrowed to 846 results for: ins

...within living cells. By coupling fluorophores to proteins, pioneers of this technique were able to directly...FRET-based biosensors, and interactions between DNA and proteins. FRET, in theory, is an easy technique, but it...to studying an unknown interaction between two proteins, these parameters are difficult to overcome but...FRET_teaching_module.pdf Day RN, Davidson MW. Fluorescent proteins for FRET microscopy: monitoring protein interactions...

...serotype that works great in a GABA neuron, for instance, may not transduce the adjacent norepinephrine...differences in tropism between animal species or even strains of the same species. What works well in a mouse...recommended to optimize to the “lowest number required” instead of using the first amount that looks effective....Moullier, P. (2002). Lack of an Immune Response against the Tetracycline-Dependent Transactivator Correlates...

...turning transcription OFF. Despite their bacterial origins, Tet systems function well in mammalian cells, ...including the “middle” ADH1 promoter pADH1m, which maintains activity during the late ethanol consumption phase...results in chimeric GAL80-repressible and GAL80-insensitive activators of lexAop. This system is commonly...modular vector for ease of use! Conclusion This post contains only a brief overview of some of the most common...

...came as an undergrad at Worcester Polytechnic Institute. Though some WPI students chose to go the solo...matter whether that other person really is acting against the team’s interests or not; once that seed of ... to be invited onto a team. Make one yourself. Instead of thinking “What’s my angle on attacking this ... Eric J. Perkins...

...scientist” conjured up images of men like Albert Einstein and Bill Nye in their lab coats handling glassware...solve global problems. Biomedical research, for instance, contributes heavily to medical practice, and ...relatives suffering from similar diseases. Others were inspired by the imminent threat of climate change to study...interesting questions and your excitement will in turn inspire the people around you. Be proactive Rather than...

...which CRISPR genome editing system you use, there remains a chance that the observed phenotype was caused...especially the clinic. Popular validation assays are insufficient As discussed in CRISPR 101: Validating Your ...that a single cell clone derived from the pool contains both the rare off target event and the desired...laboratory of Prof Steve Jackson at the Gurdon Institute at the University of Cambridge. Outside of the...

...Andel Institute. Krampus was created by Nate Levine.             View this post on Instagram                     ...today✂️❄️🦠thanks to the awesome templates by @CVRHutchinson @CVRinfo. Check them out: https://t.co/uDEE9PsspD...

...materials repository contains 139,868 reagents from 5,821 labs in 1,249 institutions across 55 countries...2,002,539 materials to 254,034 scientists in 8,900 institutions across 111 countries. Just eight days into 2024...

...with Wei Leong Chew, a researcher at the Genome Institute of Singapore who recently started his own lab....work to an independent position at the Genome Institute of Singapore. Chapter 3: Biology research and ...a Junior Principal Investigator at the Genome Institute of Singapore. His lab focuses on producing biotechnologies...

... You’ll be able to use these GIFs on Twitter, Instagram, TikTok and more. Just type “addgene” into the...looking for that perfect GIF for your TikTok or Instagram story, we also have stickers to help express your...looking for new ways to help you celebrate those wins in the lab.   You don’t need to sweep confetti...

... with “A Better Way to Share Science” for: The inside scoop from scientists who have deposited plasmids...latest technologies and breakthrough research. Insights about how to navigate through all stages of your...and Facebook. We’ll track down the story, get the inside scoop, and keep you informed.  ...

...Purification protocol page for more detailed instructions. AAV titration by qPCR using SYBR green technology...copies in a given AAV preparation. For detailed instructions and calculations on how to prepare a plasmid...study biological processes on the cellular level instead of having to work with a whole organism and can...

...minBG-jRGECO1a-WPRE3-BGHpA PHPeB Biosensor The Allen Institute for Brain Science New viral tool AiP14360:...minBG-jGCaMP8s-WPRE3-BGHpA PHPeB Biosensor The Allen Institute for Brain Science New viral tool AiP14361: ...minBG-jGCaMP8s-WPRE3-BGHpA PHPeB Biosensor The Allen Institute for Brain Science New viral tool pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE...

...aggregation of this information can provide valuable insight to the most requested plasmid, the number of requested...their CSV file from Addgene by following these instructions, and then uploading the CSV with request data...to the dashboard (Fig. 2).     Figure 2: Insert showing the upload function for the Addgene Requests...

...Here to Help Despite these challenges, Addgene remains steadfast in our mission to accelerate research...Addgene is uniquely positioned to help research institutions maximize their increasingly limited resources...investment isn't lost. Universities and research institutions can turn to Addgene as a trusted partner for...

...plasmid shipping look like at Addgene? Here's an inside peak into the daily workings in Addgene's lab. ...plasmids to more than 90,000 scientists at 5,200 institutions in 82 different countries. We continue to ship...plasmids. The partnerships and collaborations with institutions and tech transfer offices that facilitate MTAs...

...Feng Zhang (of the Broad Institute of MIT and Harvard and the McGovern Institute for Brain Research) – and... the neuroscience community pulls off such an inspiring, thought-provoking, collaborative event – all ...

...      If you’re predominantly a plasmid user, instead, don’t fret; we have backgrounds for you as well... might want to check out Addgene’s Fluorescent Proteins blog topic to learn more about FRET and other ...  Aliyah Weinstein...

... tool. A week later, researchers at the Broad Institute and MIT published another report showing Cas9'...target." As further proof of just how big and mainstream the news on CRISPR has gotten, consider these...30. (Pubmed) Programmable Removal of Bacterial Strains by Use of Genome-Targeting CRISPR-Cas Systems. ...

...position and my team at Addgene was created.”- Eric Perkins, Director of Science Product Management “I knew...mantra and never forgot it. Usually when I see inspiring artists who are thinking about getting into the... Find job listings at Addgene Get more career insight from Addgenies ...