We narrowed to 789 results for: mpl
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TypeBlog PostPublishedSept. 2, 2014, 3:05 p.m....planning and execution. I am seeing many similar examples of science trainees taking a proactive role in... Consulting. I know of many schools that have completely student/postdoc-driven biotechnology clubs such...
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Save Time and Money by Making Your Own Competent Cells
TypeBlog PostPublishedJan. 28, 2014, 3:10 p.m.... need a high efficiency of transformation (for example, if you are working with a library or an especially...making competent cells are available online. A few examples are provided here for reference: Untergasser ... -
Fluorescent Proteins 101: History of Fluorescent Proteins
TypeBlog PostPublishedAug. 7, 2017, 1:58 p.m.... of the coast of Washington state. Using these samples, he was able to isolate two proteins from the jellyfish...organisms to find the culprits. He even obtained samples of certain ones from local pet shop aquariums in... -
Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)
TypeBlog PostPublishedJuly 26, 2018, 12:46 p.m....advantage of KTRs is that they are easy to create and simple to multiplex. Problems with FRET kinase reporters...swapping of fluorescent proteins and multiplexing is simple when fluorescent proteins with limited spectral... -
Science Communication for Everyone
TypeBlog PostPublishedApril 16, 2019, 1:05 p.m.... so on, differently than scientists would. For example, “manipulate” could mean “illicit tampering” to...founder of The Addictive Brain. Prior to this, he completed PhD from Philipps-University Marburg, Germany ... -
CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping
TypeBlog PostPublishedNov. 29, 2018, 1:53 p.m....metagenomic datasets to try to determine whether simpler and maybe smaller Cas systems could exist in nature... more primitive version of the larger and more complex Cas9 and Cas12 proteins. Cas14 cleaves ssDNA But... -
Quick, Versatile Plant Transgenesis with GreenGate Plasmids
TypeBlog PostPublishedJan. 23, 2014, 3:23 p.m....cassettes for selection of transgenic plants. The complete GreenGate toolkit, now available at Addgene, also...had been humming along at 99 percent efficiency, simply stopped working. They finally traced the problem... -
Top Requested AAV of 2017: pmSyn1-EBFP-CRE
TypeBlog PostPublishedJan. 17, 2018, 2:57 p.m....provide you with that control in a modular way. For example, say you would like to label neurons with mCherry...pmSyn1-EBFP-Cre Of course there are also more complicated ways to use this particular AAV. For instance... -
Reflections and Looking Ahead
TypeBlog PostPublishedFeb. 25, 2025, 2:15 p.m....by adding long-read sequencing capabilities to complement our next-generation sequencing, setting new standards...Request, a service that dramatically expands and simplifies your viral vector options. Almost any AAV transfer... -
SciComm with the Experts at Science in the News Part 2
TypeBlog PostPublishedApril 27, 2017, 2:30 p.m.... at a local bar. It doesn't have to be crazy complicated! Listen to the full podcast for more great science... a Science Communication group started. It's as simple as getting people together at a bar. Chapter 10... -
X-CHIME: Context Dependent Germline Knockout in Immune Cells
TypeBlog PostPublishedJan. 16, 2024, 6:13 p.m....manner, but these systems have not been widely employed in vivo. The CHIME and new X-CHIME systems, developed...containing a gRNA. These transduced stem cells can be implanted in irradiated recipients to create mice with gene... -
Science as Art
TypeBlog PostPublishedMarch 2, 2015, 3:13 p.m....could provide a new tool to study old and unique samples, such as those collected by Darwin on his voyage...incompatible with the need to conserve these unique samples, meaning that we know very little about these specimens... -
What's New in CRISPR - March 2020
TypeBlog PostPublishedMarch 10, 2020, 1:15 p.m.... Blue light renders the NLS accessible and the complex enters the nucleus where dCas9-Mxi1 can repress.... In the dark, the NLS is inaccessible and the complex remains in the cytoplasm. Read the paper in Cellular... -
Addgene is Expanding Our Viral Vector Service!
TypeBlog PostPublishedOct. 15, 2024, 1:15 p.m....the service to be fulfilled and QC assays to be completed and reviewed. The timeline therefore will be as...follows: Request review: 1–2 days Prep and QC completed: 6–8 weeks Average shipping time: 1–2 days (USA... -
IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging
TypeBlog PostPublishedJune 8, 2023, 1:15 p.m....can be used with many conventional microscopes, amplified staining methods, and oligonucleotide-based staining...deep phenotyping and spatial analysis of cells in complex tissues. Proceedings of the National Academy of... -
Mapping the 4D nucleome with CRISPR/Cas9
TypeBlog PostPublishedAug. 11, 2015, 2:30 p.m....could also have major applications to cancer, for example, the visualization of chromosomal translocations...Addgene. A protein-tagging system for signal amplification in gene expression and fluorescence imaging.... -
In Living Color: The Skinny on In Vivo Imaging Tools
TypeBlog PostPublishedMarch 27, 2014, 5:14 p.m....signal-to-background ratio, thus, allowing higher resolution in samples containing substantial autofluorescence background...direction of biosensors capable of revealing, for example, how muscles work inside a living animal. For those... -
Oh, The Places You Can Go: Careers in Science Communication - Product Development
TypeBlog PostPublishedOct. 18, 2017, 1 p.m.... editing sphere.” But things aren’t always that simple, especially when your spouse is also in science... stumbled upon eScience by luck, by chance, by complete happenstance. It was on Indeed or one of the job... -
Oh, The Places You Can Go: Careers in Science Communication - Science Writing
TypeBlog PostPublishedOct. 3, 2017, 12:11 p.m....], so making it a success feels like a real accomplishment.” He started working on them shortly after ... when I am in new territory. Perhaps there are examples of science writers who can get everything that... -
Addgene's a Nonprofit? Nonprofit Awareness Day 2017
TypeBlog PostPublishedJune 5, 2017, 3:19 p.m....information.” Addgene is here to help scientists accomplish their goals. Many nonprofits must spend a lot...thought leader in scientific sharing, leading by example to increase sharing in the community Addgene fosters...