We narrowed to 813 results for: tes
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TypeBlog PostPublishedDec. 21, 2017, 2:06 p.m....detection algorithm that tolerates occasional mismatches or indels. Empirical tests indicated that a reasonable...
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The Golden Stopwatch Award: Recognizing Facilitators of Research
TypeBlog PostPublishedSept. 5, 2019, 1:06 p.m....faster with technology transfer offices (TTOs). The latest of these efforts is the Golden Stopwatch Award ...Montana State University National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK) National... -
Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science
TypeBlog PostPublishedAug. 8, 2019, 1:19 p.m....KAUST, Saudi Arabia, in the group of Prof. Mark Tester, where she focuses on (1) salt-induced changes ...Credibility, peer review, and Nature, 1945–1990." Notes and Records: The Royal Society Journal of the History... -
Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi
TypeBlog PostPublishedApril 25, 2019, 3:01 p.m....-type termini. mNeonGreen was reported as the brightest monomeric green or yellow fluorescent protein ...through successive rounds of mutagenesis for the brightest teal fluorescent proteins that were photostable... -
Fluorescent Biosensors for Measuring Autophagic Flux
TypeBlog PostPublishedJan. 22, 2019, 2:41 p.m....to the lysosome, and degradation of autophagic substrates inside the lysosome. Historically, methods for...the cytosol, this ratio is low; but when Keima accumulates in autolysosomes, this ratio is high. When untargeted... -
Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation
TypeBlog PostPublishedNov. 8, 2018, 3:47 p.m.... was natural or synthetic 97.7% of the time in a test set of 173 known synthetic and natural genes. ...saw that a percent sequence identity below 85% correlates with synthetic sequences. The researchers then... -
Viral Vectors 101: What is a Virus (and a Viral Vector)?
TypeBlog PostPublishedAug. 18, 2023, 4:15 p.m....After a virion infects a cell, the single particle creates many copies of itself, sometimes up to millions...infected cell.) This is in contrast to bacteria and eukaryotes, which use a binary replication process whereby... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog PostPublishedSept. 18, 2018, 1:33 p.m.... described by Li ZY, et al. PLoS One, 2016) and tested a series of mutations. Through this process mCarmine... mNeptune684, and has a favorable pKa of 5.6. To test out this new FP in your own experiments, mCarmine... -
Donations from Addgene to Yield Answers for Rare Disease Researchers
TypeBlog PostPublishedSept. 15, 2015, 2:30 p.m....diseases, offering them the chance to win some of the latest tools and technologies in life sciences research... other genes involved in photosensitivity in melanocytes with the goal of understanding both XP and melanoma... -
Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]
TypeBlog PostPublishedApril 7, 2015, 4:21 p.m....been limited to the overexpression of various metabolites in Escherichia coli or Saccharomyces cerevisiae...regulator or the number of designed scaffold binding sites. With hundreds of characterized aptamer domains ... -
Advice for Moving Into Sales After Your Science Postdoc
TypeBlog PostPublishedAug. 21, 2014, 3:15 p.m.... for marketing positions posted on recruitment websites, this job specifically looked for a researcher...think of to start was “Marketing for Dummies”. The fastest way to learn something about marketing would be... -
Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering
TypeBlog PostPublishedMarch 4, 2014, 4:49 p.m....neurons at once. Addgene spoke to Boyden about this latest optogenetics advance, the big questions that motivate...neuron or set of neurons, you can see how it contributes to behavior or pathology, and by disabling neurons... -
Plasmids 101: Gram Negative and Positive Bacteria
TypeBlog PostPublishedAug. 2, 2022, 1:15 p.m....layer of both bacteria types. In water, the dye dissociates into CV+ and Chloride (Cl-) ions. When the negatively...the cell purple. When the alcohol is added, it dehydrates the peptidoglycan layer, trapping the large dye... -
Early Career Researcher Toolbox: Free Tools for Science Writing
TypeBlog PostPublishedApril 28, 2021, 1:15 p.m....Pomodoro technique, which is when you spend 25 minutes focusing on a task, like writing, and then take...managers, but Mendeley is free to use. You can take notes and highlight the papers saved to your Mendeley ... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog PostPublishedJuly 21, 2017, 12:48 p.m....under a new promoter each time a new hypothesis is tested. Controlling multiple genes with Gal4/UAS system...Targeted gene expression as a means of altering cell fates and generating dominant phenotypes., Development... -
Addgene Depositors Get More Citations
TypeBlog PostPublishedApril 20, 2017, 2:30 p.m....Addgene plasmids and those without, we adjust our estimates based on the journal that published the articles... This is also true for other journals, so our estimates now take into account any differences in citations... -
The Power Behind NGS Plasmid Validation: seqWell
TypeBlog PostPublishedApril 19, 2017, 3:25 p.m.... of a 96-well plate. Then, samples from several plates are pooled together to create a single library ... from plexWell™ NGS data. Once Addgene produces plates of plasmid DNA, they are shipped to seqWell, where... -
Science Career Options
TypeBlog PostPublishedFeb. 23, 2017, 1:30 p.m....writing skills Writing a thesis or dissertation demonstrates that you have advanced writing and formatting...apply for a job, it is useful to search online job websites to see what requirements or qualifications are... -
With Our New Viral Service, We're Taking CRISPR Further!
TypeBlog PostPublishedDec. 6, 2016, 3:30 p.m....alleviate this frustration by producing, titering, testing, and providing lentiviral preps of select lentiviral... vectors from the repository for you. We perform test infections with all of our viral preps and host ... -
Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools
TypeBlog PostPublishedDec. 10, 2019, 1:58 p.m.... cell. The viral DNA containing the gRNA then integrates into the genome. Thus the gRNAs responsible for...high throughput in vivo model to identify drug candidates that modulate ApoB-LP size and concentration,...