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We narrowed to 876 results for: tra

Showing: 561 - 580 of 876 results

  1. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...blastn), Standard Protein BLAST (blastp), and Translated BLAST (blastx). NCBI has a terrific getting-started...sequences (blastn)” program for blastn so that you can extract practically every single reliable base pair from...longer alignments to compare with your sequencing trace file. Unlike megablast, the regular blastn program...gaps in the nucleotide sequence, you can run a Translated BLAST (blastx) search to check for expected ORFs..., due to the additional searches involved in translating the nucleotide sequence into all six possible...choose for your sequencing result and can easily translate it, we recommend using blastp over blastx. The...

  2. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...or Förster] resonance energy transfer). FRET occurs when energy transfer from one FP (e.g. CFP) excites...the FP (fused to the protein of interest), thus tracking the localization of that biomolecule inside living...addition to emission wavelength range, there are other traits that need to be considered when choosing an FP:...chromophore. This can be from a few minutes after it is translated to a few hours. For example, superfolder GFP ...synaptic vesicles). Some FPs have different ex/em spectra (e.g. mKeima) or change fluorescent intensity upon...

  3. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    Published
    Aug. 9, 2016, 2:30 p.m.
    ...enhancers and genome math come in. They help us to transform raw genomic coordinates into meaningful experiments...of genome math. Figure 1: Genome math illustrated. Intersect (left) and set minus (right). ... Enhancers are regions of DNA that demonstrate “spooky action at a distance.” Through the marvel of DNA...“GPS for proteins”) for NANOG, an essential transcription factor in embryonic stem cells (ESCs). Your ...H3K4me1 + H3K27ac) – H3K4me3 The following video demonstrates how anyone can find enhancers with Quilt. ...and more. We know that this matter exists, but traditional approaches to study its function have been prohibitively...

  4. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...for Biology) on Reddit and got some substantial traffic that we thought it would be a good idea. After ...resources at her disposal, she was writing all day and straight on through her designated two hours for answering... general advice on how to meet people, how to transition out of academia, and other science career topics...diverse career path development--but the science trainees still have to lead on their own development. Please...resources. The internet content for scientists in training is abundant, helpful, and sometimes excellent....Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments Additional Resources See How ...

  5. The Advances Behind The World's First Personalized CRISPR Treatment

    Type
    Blog Post
    Published
    Sept. 16, 2025, 1:15 p.m.
    ...disorder survive long enough to receive a liver transplant — creating intense time pressure for the research...al., 2017). These editors did not create double-stranded breaks in the DNA backbone, making them significantly... repaired with an A→G edit on the complementary strand. But the invention of base editing doesn’t necessarily...cause off-target effects in a variety of RNA transcripts independent of Cas9 targeting (Rees et al., 2019...of a target base in genomic DNA without double-stranded DNA cleavage. Nature, 533(7603), 420–424. https...Whittaker, M. N., & Kleinstiver, B. P. (2020). Unconstrained genome targeting with near-PAMless engineered...

  6. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...need. Most often, early career scientists aren’t trained to have these types of negotiations. They usually... centrifuges, sequencers and analyzers, and any ultra sensitive equipment (RT-PCR, Microscopy, Fluorescence...list from your current P.I., lab manager, or administrator containing all of the materials that you’ve ...searches, and seek advice from your current administrative services, managers, and techs’ financial books...to proceed with a used or new item. To further illustrate this point, if your research is dependent on ...Channel your inner curiosity and use your research training to help you find other solutions to reach your...

  7. Addgene Stands with the Scientific Community

    Type
    Blog Post
    Published
    April 23, 2025, 1:30 p.m.
    ...developments. These abrupt changes threaten to slow transformative biological discoveries and life-saving medical... can prove to be vital during this period of constrained funding. Addgene is uniquely positioned to help...archiving their biological materials during funding transitions. Enabling resource sharing: Our operating model...independently — particularly valuable when budgets are constrained. This collaborative approach eliminates redundant...Throughout history, the scientific community has demonstrated remarkable resilience and creativity in the ...

  8. A Scientist's Guide to Ebola

    Type
    Blog Post
    Published
    Oct. 16, 2014, 3:01 p.m.
    ...characterized by single, linear, negative-sense, single-stranded RNA genomes. The virus does all of its damage ... also recently described how the glycoprotein transforms itself to escape vesicles within host cells and...critical component of vaccines and the target of neutralizing antibodies and inhibitors of attachment and ...California, Santa Cruz, offers sequence data on five strains of Ebola representing the latest outbreak and earlier...the current Ebola crisis, Science and Science Translational Medicine have made their complete collection...

  9. What's New in CRISPR - May 2019

    Type
    Blog Post
    Published
    May 28, 2019, 12:49 p.m.
    ...Gordon's lab developed a strategy to enhance HDR repair using a single-stranded oligodeoxynucleotide HDR...single-AAV delivery of Nme2Cas9 and the sgRNA to demonstrate efficient editing in adult mice and in zygotes...Shapiro’s lab. After testing dCas9 fusions with transcriptional repressors, they found that fusing dCas9 to...to an Mxi1 repressor domain repressed transcription of HSP90 an essential C. albicans gene. Read the ...

  10. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    Published
    Dec. 30, 2015, 3:30 p.m.
    ...-9 secretion, and identifying lysine methyltransferase substrates. We’re incredibly happy that lentiCRISPR... name suggests, lentiCRISPR v2 is a lentiviral transfer vector used to deliver both Cas9 and your gRNA...343.6166 (2014): 84-87. PubMed PMID: 24336571. PubMed Central PMCID: PMC4089965. 2. Sanjana, Neville E., Ophir...(2014): 783-784. PubMed PMID: 25075903. PubMed Central PMCID: PMC4486245. Resources on the Addgene Blog...

  11. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    Published
    Dec. 29, 2020, 2:15 p.m.
    ...complement our RNA Extraction Without A Kit blog post, we've compiled five methods for extracting DNA without...sharing even broader and began working with tech transfer offices to make reagents available to scientists...that cause plasmids to be incompatible. 4. RNA Extraction Without A Kit by Leah Schiesow We added to our...it, wrote these tips for working with RNA and extracting RNA without a kit. 3. SARS-CoV-2/COVID-19 Detection...

  12. CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping

    Type
    Blog Post
    Published
    Nov. 29, 2018, 1:53 p.m.
    ...containing the putative tracrRNA into a plasmid and expressed it in an E. coli bacteria strain. They found that...domain, characteristic of CRISPR-Cas enzymes. In contrast to other Cas enzymes, Cas14 has not been found...that Cas14 could indiscriminately cleave ssDNA in trans like Cas13 and Cas12 do for RNA and dsDNA, respectively...can cleave fluorescent probes of dsDNA and RNA in trans. Fluorescent signal indicates the presence of this...

  13. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    Published
    June 17, 2014, 7:57 p.m.
    ...ability to direct enzymes – from nucleases to transcription factors – to specific sequences of DNA has become...and her colleagues led by Zhao described and demonstrated in a report published in The Journal of Biological...Biological Engineering in March is fused to a translational repressor. In principle, however, designer PUF...on a version that she hopes will change the intracellular localization of RNA. “You can think of changing...of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014...

  14. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ...GFP spreads through the cytosol via cytoplasmic strands. Image from Sattarzadeh et al., Sci Rep. 2015 CC...signal. Red signal intensity increases with GFP concentration and saturates around 30 uM. For low-abundance...most useful for labs that already have created a transgenic GFP-containing organism,” says Hanson, “using...unexpectedly photoconvertible GFP proteins to make a new transgenic line could avoid problems in localization that...Green to red photoconversion of GFP for protein tracking in vivo. Sci Rep. 2015 Jul 7;5:11771. doi: 10.1038...

  15. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    Published
    June 19, 2015, 3:08 p.m.
    ...plasmid construct, most often in special E. coli strains designed for protein expression. Cultures are then...glycosylate proteins and may recapitulate the post-translational modifications added to proteins in other eukaryotes...the MISTIC (membrane-integrating sequence for translation of integral-membrane protein constructs) protein...(2014): e112643. PubMed PMID: 25386923. PubMed Central PMCID: PMC4227841. 2. Young, Carissa L., Zachary...Resources on the Addgene Blog Plasmids 101: E. coli strains for protein expression Plasmids 101: Protein tags...

  16. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    Published
    Feb. 18, 2015, 3:09 p.m.
    ...details how to transfect CRISPR plasmids into MEL cells by electroporation, sort your transfected cells via...genomic deletions We were inspired to produce intrachromosomal deletions based on the experiments of Kim ...CRISPR/Cas9, we began to explore the paired double-strand break (DSB) approach at a variety of loci. We were...Targeted deletions are just one of many available strategies by which biologists can imagine, create, and ...

  17. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ...life repair genomic damage, specifically double strand breaks. Researchers have long taken advantage of...product is introduced into the cell by direct transformation. A given insert will typically contain both...selectable gene product for isolation of successful transformants. Addgene distributes several ready-to-use, modular...native conditions and allow scientists to not only track the abundance, movement, and localization of individual...markers for combining modifications within a single strain. In addition to the collections featured above,...

  18. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

    Type
    Blog Post
    Published
    June 23, 2020, 1:15 p.m.
    ...worked closely with the legal partners and Tech Transfer Offices at universities to make COVID-19 research-related...United States, and many universities and Technology Transfer Offices were, at least, temporarily closing, these...scientists, we’ve worked with many scientists, tech transfer offices, research foundations, and funding initiatives...them available to industry, please reach out at techtransfer@addgene.org and we will initiate a conversation...conversation with you and your technology transfer office to discuss the possibilities for expanded sharing. ...

  19. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ...many applications, the most well-known being transcriptional activation/repression. dCas9 has also previously...could then be used to examine both inter- and intrachromosomal dynamics, and how they change in response ...turned their attention to mapping pairs of intrachromosomal loci. The technique successfully resolved ... this work represents the first mapping of intrachromosomal loci, a major benchmark in characterizing ...for example, the visualization of chromosomal translocations or chromosomal shattering (chromothripsis)....

  20. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...vector design. B) Strategy for leak-free transcriptional control. C) Iterative transduction workflow overview...organoids. The Organoid EasyTag System is a straightforward workflow for doing gene targeting, reporter...specificity for SLs in vitro, and they further demonstrated that when tested in tobacco protoplasts, the...
Showing: 561 - 580 of 876 results