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We narrowed to 790 results for: Cre

Showing: 21 - 30 of 790 results

  1. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...transferable skill: creativity. I’ve heard quite a few scientists state that they’re not creative, usually saying... artistic, and therefore not creative. However, if we think of creativity as a form of innovation, it’...clear that creativity is absolutely a transferable skill developed during PhD training. Creativity is required...innovation. If you identify as a non-creative or if you find yourself in a creative rut, read on!   Fig ...had to think creatively to solve a problem. But how do you intentionally engage in a creative problem-solving...Grant, a creativity expert at Stanford conducted an experiment in which people were asked to create a device... 2: Blugene likes to spark creativity in out on the open sea Creativity comes in many forms. Whether...

  2. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...variety of screens, including interference, activation, and knockout. Most CRISPR screens rely on lentiviral..., AAV vector screens may be AAVantageous!       In vivo, in vitro, ex vivo CRISPR screens can be carried... Figure 1: Overview of in vitro AAV CRISPR screens. Created with BioRender.com. The in vitro option... For in vivo screens, mice are directly infected with the CRISPR library. In vivo screens can become costly...engineered in labs. This vastly increases the possibilities when designing screens. Each serotype has its own...CRISPR Delivery Genome-wide Screening Using CRISPR Performing In Vivo CRISPR Screens Using the FITS Approach...Forward genetics screens are a valuable part of the molecular biology toolbox to identify new target ...

  3. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...provide informative content to help researchers in creating and sharing knowledge, related to the types of...Identify needed content Let’s take a look at how we create this content, using the example of our Antibody...Tweet, followed by very detailed answers from our incredible scientists. Expert reviews on blog posts are ...need to reach out to an external expert to help us create accurate content. Regardless of the process, everything...

  4. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ...right for you. Screen variations Forward and reverse screens Forward and reverse screens involve manipulating... suppressor screens These types of screens are very similar to forward and reverse screens, except that...approaches. Screens and selections The common goals The confusion in differentiating between a screen and a ...reverse screen – all known genes are assayed for their role in a particular capacity. Drug screens, on the... time screening hits that aren’t “real” or novel for their purposes… or worse yet have a screen that doesn...phenotype. These screens employ the same downstream manipulations as a forward or reverse screen (CRISPR, drug...component. Alternative screening techniques While CRISPR and drugs dominate the screening scene, there are ...

  5. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...genome-wide screens? The goal of a genome-wide screening experiment is to generate and screen a population...transfer vectors to create the CRISPR library. CRISPR libraries have been created to knock out, activate...the steps involved in a CRISPR screen? Performing a forward genetic screen using CRISPR libraries is a multi-step...sorting and screened for a particular phenotype. For example, mutant cells can be used in drug screens to identify...means to carry out so-called “forward genetic screening experiments”, it is certainly the most robust....libraries are being used to perform genome-wide screens and highlight some of the reagents that have been... different genomic target. The goal of a CRISPR screen is to use Cas9 and a pool of gRNAs to identify ...

  6. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ...insert (blue-white screening). Other types of screening methods Although blue-white screening is probably the... that! (To read about other screening strategies, check out our screening overview blog post.) *Note:...good, but how does this help us screen plasmids? Blue-white screening in the lab Scientists discovered... few common vectors. Blue-white screening is just that – a screening process. It does not select only...can talk about an even more specific method of screening your cloning reaction. Being able to select for...Note: Two Popular Vectors for Blue White Screening Available at Addgene Are: pUC18 and pUC19 Let’s begin ...from the lacZ gene (a mutation called lacZΔM15) creates a non-functional β-galactosidase enzyme. Providing...

  7. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ...to provide them to their students. My desire to create a set of low-cost, easy-to-use educational biology...fragrances that they can smell, or enzymes that can create hydrogels that they can touch. With BioBits, we...

  8. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...CRISPR knockout screens: Full genome vs. custom screens Full genome CRISPR knockout screens have been used... gRNAs in a screen, to ensure that each gRNA has a good chance to contribute to the screen. In the end...Secondary screens  If you have generated a list of candidate genes from a full genome screen, a smaller...genes you’d like to investigate in your next CRISPR screen. Luckily, the Green Listed software can help you...software tool used to design gRNAs for custom CRISPR screens targeting a (long or short) list of genes provided...fantastic tools for unbiased discovery. With these screens, the investigator generates a cell population where...involved in the studied phenotype. For robustness of a screen, each gene is not targeted by only one gRNA, instead...

  9. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    Published
    March 30, 2017, 2:30 p.m.
    ...in a sample. Digital PCR assays for mutation screening Now that we have introduced how digital PCR works...the bi-allelic mutations typically desired when screening clones for complete functional knockout of your...interested in harnessing the power of digital PCR screening to validate your genome edits, this paper provides...Remember that although digital PCR is a great screening tool, it still requires target DNA sequencing ...PCR-Based Method for Efficient and Highly Specific Screening of Genome Edited Cells. PLoS ONE 11, e0153901–...

  10. 5 Science Rap Videos & Their Creator: Tom McFadden

    Type
    Blog Post
    Published
    Feb. 9, 2017, 3:30 p.m.
    ...worked with high school students in the bay area to create a plasmid rap video for us (If you’re new to plasmids...unfortunate story of Rosalind Franklin and her incredibly important role in solving the structure of DNA...is a YouTuber and science educator looking for creative ways to implement the next generation science ...
Showing: 21 - 30 of 790 results