We narrowed to 291 results for: Fus

...beta-arrestin-TEV protease fusion to a receptor-transcription factor fusion. TEV protease cleave the transcription... et al., 2017). In this technique, the receptor fused to APEX2 catalyzes the biotinylation of proteins...

...proteins into one particle within the cell, the lab fused the red fluorescent protein (RFP) to a self-assembling...microcompartment protein. The expression of this fusion is under control of the arabinose-inducible promoter... with arabinose to begin expressing the SAP-RFP fusion. Then, the cells are washed to stop the production...

...interference (CRISPRi) targets a catalytically dead Cas9 fused to a transcriptional repressor to your gene of interest...activators (CRISPRa) are transcriptional activators fused to a dead Cas9. The system is led, via a custom ...specific ligand to mark the tag and whatever it is fused to for degradation. Since the tagged protein of ...

...showed that nanobodies could be fused to other proteins, and these fusions retained the ability to bind ...Caussinus, E., Kanca, O., and Affolter, M. “Fluorescent fusion protein knockout mediated by anti-GFP nanobody....

...protein-protein interactions. One popular approach is to fuse a protein of interest to each part of a split fluorescent...General Design Figure 1: Two proteins are fused to the FP(11) and FP(1–10) fragments. When Protein...cell environments). Also consider that a protein fusion may disrupt functionality or binding of your target...

...  Confirming tags and fusion proteins Finally, we confirm promoters, tags, fusion proteins, and selectable...NCBI entry or using BLAST.   Confirming tags and fusion proteins. We’ll break down each of these steps...

...our FRET Resource page and can be used to create fusions with a gene of interest. These plasmids will be..., where the donor and acceptor fluorophores are fused to two separate proteins. (In contrast, intramolecular...achieve, because the ratio of acceptor to donor fusions varies with transfection efficiency and any unpaired...

...replace” genome editing technique that relies on a fusion enzyme of Cas9 nickase + reverse transcriptase ...Nelson et al., 2022). PE7: This editor includes the fused RNA-binding protein La to protect the end of the... not epegRNAs) can improve either endogenous or fused La binding (Yan et al., 2024). Figure 9:...

...with Peptide-Assisted Genome Editing (PAGE). They fused the Cas enzyme to cell-penetrating peptides and ...guides. Figure 1: A) Cell-penetrating Cas9, fused to HIV TAT, Myc and SV40 Nuclear Localization Signals... et al. (2023). StayGold variants for molecular fusion and membrane-targeting applications. Nature Methods...

...co-expressed with a fusion protein containing VP2C and a protein of interest (POI). The VP2C fusion protein anchors...while still allowing substrates and products to diffuse freely into or out of the protein compartment. ...

...TIRF-SIM imaging of COS-7 cells expressing LifeAct fused to mVenus, mGold2s, or mGold2t. Bottom: Quantification...synapses (Rivera & Huang, et al., 2025). AF was fused to a presynaptic vesicle protein (VAMP2) and an ...remain on the presynaptic cell membrane while AF, fused to a payload, is released into the synapse. Once...

...2: CLIP-SNAP fusion proteins for single-molecule FRET. A) Schematic of a CLIP-SNAP fusion protein (a.k.a...YFP-tagged G protein and a NanoLuc® (Nluc) Luciferase-fused detector that specifically binds to active (GTP-...

...Cas break for HR is to fuse an HR protein to the nuclease. Early HR factors fused to Cas9 have been shown..., A. H. Y., Menerot, S., Brion, A., et al. CtIP fusion to Cas9 enhances transgene integration by homology-dependent...

...integrase via protein fusions, using Prime to lay a “beacon” into the genome that a fused integrase would then... single protein, computationally described as a fusion of four Cas7 proteins and a putative Cas11-like...

...live cells. Indeed, groups have utilized dCas13 fusion proteins for imaging, tracking, modulating splicing...Leptotrichia wadei (LwaCas13a), using a superfolder GFP fusion to stabilize the protein in mammalian cells. LwaCas13a-msfGFP...programmable RNA-binding protein, creating the fluorescent fusion protein dLwaCas13a-NF for in vivo RNA imaging. ...

...colleagues devised a “Modular fluorescence unit fused with TRansmembrane region-to-IntrAcellular loop ...immunoreceptor tyrosinase-based activation motif (ITAM). By fusing ITAM to an RTK of interest and co-expressing a ...to meet those requirements (Lu et al. 2022). By fusing two bright fluorescent proteins with different ...

... Learn about in vivo biotinylation of bacterial fusion proteins Biotin is an essential vitamin for life...protein of interest with biotin by simply creating a fusion in which AviTag is added to either the N or C terminus...Addgene blog In vivo biotinylation of bacterial fusion proteins Learn how biotin is used in Split-BioID...

...tetracycline-controlled transactivator (tTA). They fused TetR with the transcriptional activation domain ... A chimeric transactivator, cTA, was created by fusing CymR to the activation domain VP16. cTA binds to...with LexA binding to the lexA operator (lexAop). Fusing the LexA DNA-binding domain (DBD) to GAL4 or VP16...

...such as inducible expression of the hpRNA and/or fusing your gene target sequence to a fluorescent reporter...of the DNA construct, where your RNAi-target is fused to the DNA backbone that contains a target sequence...achieved this by creating constructs that directly fused any gene targeting sequence to a targeting sequence...

...are artificial restriction enzymes generated by fusing a zinc finger DNA-binding domain to a DNA-cleavage...to introduce point mutations in the genome. They fused a catalytically impaired Cas9 protein to a DNA-editing...in 2019. It’s based on a prime editor which is a fusion between Cas9 and reverse transcriptase. Using an...

...Belzung et al., 2014). This could be overcome by fusing opsin genes to cell-type specific promoters; however... an opsin gene (e.g., channelrhodopsin-2, ChR2) fused to a reporter gene (e.g., mCherry) to easily detect...detect opsin-expressing cells. The resulting fusion gene is inserted in the antisense orientation with ...

...as a fusion protein. An ScFv is made up of the variable regions of the heavy and light chains fused together...produced from hybridomas. Hybridomas are formed by fusing B cells that have been extracted from animals injected...

.... Empty backbones often contain epitope tags or fusion proteins that are appended to new inserts through... the gene cloned into your plasmid, any tags or fusion proteins contained in the plasmid, any mutations...affinity for calcium". Please describe any tags or fusions (e.g. His, FLAG, EGFP, etc) on your insert. Use...

...of traditional tracing compounds is that their diffusion across the synaptic cleft is usually severely ...small (20 nm) in size which makes them capable of diffusing in the extracellular space; they also have low...there is always a risk that viral particles will diffuse long distances within the extracellular space along...

...surface – making it critical for viral attachment and fusion. Once inside a host cell, new evidence in PLoS ...neutralizing antibodies and inhibitors of attachment and fusion. Recommended Reading For those looking to delve...

...Plant base editing with a Cas9-adenosine deaminase fusion Caixia Gao’s lab has developed a new plant base...from an evolved tRNA adenosine deaminase that is fused to a nicking Cas9. This base editor can be used ...

... is fused to a translational repressor. In principle, however, designer PUF proteins can be fused to other...

... and A-to-G base editors into one protein. They fused an activation-induced cytidine deaminase (AID) and...adenosine-to-inosine (A-to-I) RNA editor called REPAIR by fusing catalytically inactivated RNA-targeting Cas13 (...

... e67902. Many imaging studies rely on direct fusion of fluorescent proteins (FPs) to a yeast gene of...epitope tag for creation of your own unique protein fusions. This collection is ideal for tandem affinity purification...

...making fusions, see this blog post from Joachim Goedhart: Fluorescent Proteins 101: GFP Fusion Proteins...

...The first functional SB system was synthesized by fusing and modifying various extinct transposases. Improvements...is made up of a fragment of the RPL13a promoter fused to a region of the RPL41 gene, to drive the selection...

...these applications, however, nuclease-dead Cas9 is fused to a transcriptional activator or repressor to create...libraries In surface display, proteins of interest are fused to anchoring proteins to be expressed on the surface...

... common protein-only transduction strategy is to fuse a cell penetrating peptide (CPP) to a protein of...discovered in the HIV protein Tat. Although CPP fusions can translocate a cell membrane, the presence of...

...lab first thought to enable MBE by using Cas12a fused base editors (dCas12a-BE). However, the poor editing...efficiencies they found led them back towards Cas9 fused base editors (nCas9-BE), as Cas9 is mechanistically...

...of dCas9, a gRNA fused to an RNA binding motif, and a RNA-binding protein-bEFP fusion. Thus, the bEFPs...

...useful for studies in non-human primates, where fusion fluorescent proteins can affect DREADD trafficking... (PV2527) 105679  AAV5 ppAAV-CKIIa-stGtACR1-FusionRed New tetracycline transactivators Plasmid...

...in human cells (Ma et al., 2015). To do so, they fused a different fluorescent protein to various Cas9 ...Cas9 variants, SpCas9, NmCas9, and St1Cas9. These fusions localized to specific loci based on the guide RNA...

...with other pathways. Since these effectors are diffusible, they’re also not suitable for spatially limited...light-sensitive two-component system (TCS). Levskaya et al. fused a photosensory domain from cyanobacteria to a common...

...not appropriate for mammalian selection – do not confuse this with G418 (aka Geneticin). Neomycin should...mammalian expression – instead use G418. This can be confusing since the neo/kan gene confers G418 resistance...

...2021.    BLITZ uses the biotin ligase TurboID fused to a conditionally stabilized GFP-nanobody rather...rather than a specific protein of interest. This fusion protein can then be co-expressed in essentially any...

...cryopreserved hybridomas, immortalized cells created by fusing an activated B cell that produces the antibody ...light (VL) and variable heavy (VH) chain sequences. Fusion PCR was performed to create an amplicon that  was...

...unit. Each particle has a viral envelope, which fuses with the target cell’s membrane, allowing the viral...determine what type of cell the virus will target and fuse with. In nature, this determines which organisms...

...luminescent reaction when they come together. By fusing each luciferase subunit to other proteins, researchers...A luciferase and a fluorescent protein are each fused to one of the proteins being tested for interaction...

... Screens and selections The common goals The confusion in differentiating between a screen and a selection...inappropriately categorized as screens! Why the confusion? The commonality between these methods is that...

...components: 1. A nucleolytically inactive Cas9-VP64 fusion; 2. A sgRNA incorporating two MS2 RNA aptamers ..., which Konermann recognized as an ideal spot to fuse the activators instead of at the protein’s C terminus...

...catalytically inactive cas9 (e.g. dCas9) has been fused to a variety of protein modules to repress or activate...consist of the GAL4 or ZIF268 DNA binding domains fused to either the hER or hPR ligand binding domains ...

... monitor this activity, Pedre Pérez’s biosensor fuses MPST to roGFP2. Because roGFP2 contains two cysteines...chemogenetic agonist inhibits the neuron (via hM4D). She fused fluorescent protein tags to the receptors to monitor...

...I suspected I knew at least one source of the confusion in our meetings. A lot of our LIMS planning involved...can help the team identify possible points of confusion in the future. Improving cross team communication...

...   Prior to the polyhistidine tag, alternative fusion tags were used, such as S. aureus’ Protein A (Moks... to scientists. If their protein of interest is fused to polyhistidine, then they can use an anti-HIS ...

... with soluble fluorescent proteins or localized fusion proteins and do not require dedicated plasmids ...performed on purified fluorescent proteins. To avoid diffusion, the proteins are (i) trapped in microdroplets...