We narrowed to 528 results for: Set

...DNA Cross-Bridging Shapes a Single Nucleus from a Set of Mitotic Chromosomes. Samwer M, Schneider MWG, ...

...fluorescent pH sensors, a set of luciferase-fluorophore pH fusion reporters, pRSETb-pHlash and pcDNA3.1+-pHlash...obtained through Addgene: Rapamycin-inducible Cas9 sets (Addgene plasmids 62883 &62884; 62885 & 62886) Rapamycin-inducible... Rapamycin-inducible dCas9-VP64 activator set (Addgene plasmids 62887 & 62888) Zetsche et al., Nat ...the MIT Synthetic Biology Center have deposited a set of 11 phage integrases which act irreversibly on ...model systems. The MultiSite Gateway Kit contains a set of 12 promoter-less Gateway® entry clones to be used...now available at Addgene, so on your marks...get set...RUSH. Boncompain et al., Nat Methods 2012. Mar...; the Cell-free expression test kit represents a set of 88 clones in a pCellFree vector that enables protein...

...around the scientific world, so you might wonder what sets HiBiT apart. While the classic Green Fluorescent...and cellular autofluorescence (Tsien, 1998). What sets HiBiT apart from these tags is its use of bioluminescence...explore TP53 biology. They first identified that in a set of patients with gastric cancer (GC), TP53 was one...early-onset GC and one with a mutation at R273H to match a mutation frequently found in late-onset GC. ...Site-Linked Chemosensitivity in Early- and Late-Onset Gastric Cancer. Cancer Medicine, 14(8). https://...

... based on the identity and order of a particular set of inputs, may be used to better understand and engineer...on the state cannot be undone and also limit the set of possible downstream states that can be achieved... register to record all permuted substrings of a set of chemicals. Specific examples of arrangements that...possible to build gene regulatory RSMs (GRSMs, the subset of RSMs that regulate gene expression) that uniquely...

...or circuits with functional relevance following a set hierarchy. MoClo originally developed from Golden...unpredictable cases, MoClo provides two answers: first, sets of parts with characterized behaviors are available...

...simply pause and take a deep breath before the panic sets in. Most of the time, that’s enough–but if it’s ... lab meetings, you’ve developed a valuable skill set that will be useful in nearly any other job you may...

...variety of transferable skills. At Addgene, we've set out to fill this gap by both highlighting that researchers...being able to recognize it as a part of your skill set later on. If you are new to research and the lab,... define this skillset. After sifting through dozens of career advice articles, I settled on defining problem...qualified to be an astronaut. But, knowing that this skillset is a part of your toolkit is important. Paired...

... many of the same elements as a STAR answer: a setting, a conflict, and a resolution. Stories usually ...interviewers will not have the same experiences as you, so set them up for success by giving them sufficient background...

...Whenever an order is placed on the Addgene website, it sets off a chain reaction of events throughout all of...dealing with the samples to newer lab members. We can set ourselves up for success by giving explicit instructions...one of these steps can fail but, in our current setup, there are no explicit instructions on what to do...but critical logic. Some steps fail “hard”, they reset the whole procedure. Other steps fail “soft” they... and capital cost of getting up to speed with a setup is almost always worth it. Because labels are applied...

...paper from Ed Callaway’s lab (Fischer, KB. 2019) set-out to systematically test the potential causes of...expression in vivo.  Since DIO/FLEx vectors contain two sets of homologous recombination sites, they hypothesized...should now be able to make smaller adjustments to a set of injections into your animal model. For example...of AAV vector we produce containing a DIO/FLEx cassette using next-generation sequencing (NGS) (Guerin...

...CRISPR knockout screens have been used in several settings, and are fantastic tools for unbiased discovery...library before using them to understand how they are set up. Using the “User Upload” alternative, you furthermore...convenient for you to later identify how you were setting up the experiment.  Output_NotFoundList: A text...approach is to use smaller libraries targeting subsets of genes. With such an approach, you lose the unbiased...differentially expressed genes found in a gene expression dataset are causal for a phenotype of interest. Selected...

...may generate irreproducible data in a research setting, but these events could have disastrous health ...criteria are not exhaustive. Mismatch tolerance settings are often limited to off-target sites of <4 bp...clinical approval. It is key to maintain a consistent set of standards as the field seeks to generate reproducible...streptavidin and next-generation sequencing (BLESS) (Crosetto et al. 2013). GUIDE-seq and BLESS detect double-stranded...25503383. PubMed Central PMCID: PMC4320661. 14. Crosetto, Nicola, et al. "Nucleotide-resolution DNA double-strand...

...summer role was incredibly valuable because it was a set duration of only three months and made a good job... in a developing tropical country. This is why I set up a placement working in a rural health clinic in... can be a glut of individuals with similar skill sets. This is not to say that all commercial companies...

...polymerase, and a DNA ligase to carry out a pooled set of ligations in an hour or less. The ligations combine...allows one to simultaneously complete an ordered set of scarless ligations following digestion with Type...

...well-studied and inexpensive. In addition, a complete set of well-annotated deletion strains is available (...content knowledge and research skills (8). A complete set of student and instructor resources is available ...

...options depending on above selections) Module set (the set of components you want in your vectors) Target... to switch out reporters, Cas proteins, guide cassettes, promoters, N’ and/or C’ terminus, and selection...destination vectors contain an ampicillin resistance cassette and an origin of replication, which are not schematized...

...published papers that contain that term. You can also set up alerts for new publications from a particular ...similar to Facebook or Instagram. Key features Setting up a personalized feed is simple. You can enter...

...follow-up DNA oligos to arrive. We also were careful to set up clear tasks for each member of the team to accomplish... can detect the virus in a clinically relevant setting. Molecular diagnostics for the Zika virus predominantly... the work done as quickly as we could. From the outset, we spread out the risk for the project by initially...

... as the overall alignment results. The default setting is “Optimize for Highly similar sequences (megablast... it saves analysis time to remove this default setting. Timesaving Tip #2: blastx searches are inherently...

...structural plasticity in single dendritic spines. The set of CyRFP constructs available at Addgene includes...illustrates the utility of PhoCl in a variety of settings. For instance, inserting PhoCl in between a protein...molecular weight markers The Tan lab at Penn State - set out to “rule” Molecular Biology. Under the supervision... of Minnesota recently deposited a comprehensive set of plasmids that can be used for either TALEN or ...kanamycin cassettes and were carefully designed to allow for easy removal of the resistance cassette via Cre...Every quarter we highlight a subset of the new plasmids in the repository through our hot plasmids articles...the transcription factors from a polycistronic cassette. However, it was unknown whether variations in...

...technology Here’s some advice on how to successfully set up your qPCR to determine the genome copies in a ...

... of the environment of the department, the lab setting, and how students truly feel about the program ...stressful time, and I was more relaxed during both sets of interviews. I also had universities change their...

...variety of transferable skills. At Addgene, we've set out to fill this gap by highlighting that researchers...professional negotiation look like? In professional settings, creative negotiation skills are key to maintaining...get what I want, that means you don’t. In this mindset, one of us wins, the other loses. Although this...

...applications. These PCR methods all use the same basic PCR set up and steps but differ in how the PCR products are...amplified using several forward and reverse primer sets.  Multiple template PCR - multiple templates with...

...of wild-type TadA and TadA7.10, they designed one set of ABE8’s similarly (ABE8.x-d). In order to assess...from the complex the researchers developed a second set: The ABE8.x-d version carries a fusion of wild-type...

...one set of genetic loci can be introduced together with truncated gRNAs targeting a different set of loci...

...proposing new programs, we're always striving to set an example of environmental stewardship.  Additional...

...have skill in a particular technique? Do you have a set of tips you give to every new lab mate? Do you have...

... and their team at Calico Life Sciences recently set out to do just that — by developing a new fluorescent...response. Evolution of a new variant Maria and the team set up a directed evolution and screening experiment ...

...variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting that researchers...doesn’t end after you get your first new job. To set yourself up for success in the career you hope to...

...variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting that researchers...!). Then schedule a meeting to discuss the work, set expectations, answer any questions, and (most importantly...

...that can be difficult to replicate in other work settings: The sense of being the first to discover something...academia provide a formal training regime and the right set of tools to help you do your job as well as possible... find the idea of not holding a pipette again upsetting, there are ways to get back to the bench during...

...way to develop and exercise your scientist skill set. If you dread the thought of writing a review, or...I was by no means an expert on this topic when I set out to write the review. That’s just to say that ... isn’t possible. So, define your scope from the onset. Perhaps you, a colleague, or your adviser was invited...

...cloning technique that will work well with a given set of resources, time, and experimental needs. Since...restriction and ligation cloning. It is also possible to set up the BP and LR reactions in the same tube, speeding... create an entry clone: Restriction cloning the inset into an attL-containing entry vector.   Pro...

...communicating ideas in a content-rich, easy-to-understand set of PowerPoint slides. What are some of the more challenging... biology or medicine you will bring an essential set of tools to a consulting team. Your case team will...

...will need to grow this initial stock and create a set of vials of ‘Master’ stock, which can be stored in...Master vial can then be thawed and used to produce a set of ‘working’ stocks of cells. Working stocks should...

...order to try and access the entire interactome of a set of proteins at once.  However, as Y2H screens are...positive and false negatives, these experiments must be set up, conducted and analysed with care to get reliable...entire proteome of a particular cell type, or a subset based on a cellular compartment or growth stage... as a good positive control to ensure that your setup works. The last thing you want is to run your lovely...

...plenty of events that included a simple corn hole set and a cooler. Chances are one of your colleagues ...especially true. If your event requires a lot of set up or cleanup, ask for volunteers to help out. Delegate...

...make sense of it, with their decision to publish setting the clock for others to dive in. The real cost ...usually condensed into a statistically evaluated set of values.  However, to evaluate the interpretation...anonymous request.  This means that supporting datasets must be logically annotated and arranged, as in...should be primarily responsible for storage.  If datasets are too large to transfer to a journal repository...peer-reviewed repository for “scientifically valuable” datasets. It likely won’t be long before other data repositories... move towards encouraging authors to integrate datasets with their resultant figures. Instead of providing...

...hoarding used tip boxes for a lab supply tree and set a challenge to my lab mates. They did not disappoint...

...Blog Series We have not one but two new blog series set to launch this summer! Summer SciComm Series From...

...scavenger hunts. In fact, they’ve been following a set of clues that has led them to their biggest discovery...

...is an integral part of any bacteriologist’s skill set. So when Dave Westenberg taught this concept in his...the streak plate challenge. Chromoproteins are a subset of the fluorescent protein family that give off...

...or "Unpublished or pre-publication plasmids" and set a desired distribution status. You can submit "Published... below:   Pooled Library A pooled library is a set of plasmids all built with the same backbone and ...entered will be saved and the page will open a new set of entry fields to describe the next insert. Repeat...

... super family). To overcome these challenges, we set out to develop a hassle-free, highly efficient Cas9...eLife 2018;7:e35069 DOI: 10.7554/eLife.35069. How to set up csRNP knock-in experiments If your lab is new ...experiments, there is no need to worry at all. Setting up tag knock-in experiments using the csRNP method...

...by the lab of Joachim Wittbrodt might be just the set of tools that you need. The Golden GATEway cloning...which signaling events occur in a physiological setting. Genetic techniques typically rely on overexpression...RF-1; Bacterial strain EcAR7) and minimizing the set of plasmids required to make singly or multiply phosphorylated...between. The MoClo system is comprised of three sets of cloning vectors (Level 0, 1, or 2) which can ...Additionally, the lab recently described the use of another set of mGRASP plasmids in their Neuron 2014 paper. ...sequence to be integrated and a removable integrase cassette. They validated this methodology in two differents...expression and purification of Cas9 protein in Rosetta E. coli. New Lentiviral CRISPR activator and repressor...

... with GFP Kevin White’s lab recently deposited a set of CRISPR plasmids to tag human transcription factors...

...with the complete #MicrobeAdventCalendar ornament set (plus more!) It features archaea, bacteria, fungi...

... are sharing what they call “raw science” – data sets, code, and, I think it’s safe to say all of us here...

... kills you, but the cytokine storm it ultimately sets off.  The virus works its way into immune cells ...