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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 120 results for: ALC

Showing: 41 - 60 of 120 results

  1. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ...the transfection method (i.e. polyethyleneimine, calcium phosphate or liposomal methods), the volume of ...be optimized prior to starting. We recommend calculating the ratio of the surface area of the larger vessel...

  3. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...to your normal control - you can set up your calculations so that your assay output number (RQ value) ... determination of amplification of Plasmodium falciparum multidrug resistance-1 gene (pfmdr1). J Parasitol...

  4. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...vectors expressing GCaMP6f with this promoter. Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f...terminal providing better signal to noise for imaging calcium dynamics in fine structures. We have expanded our...

  5. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...qPCR Protocol for more details. Pro Tips When calculating the titer for your AAV, remember to account for...tool for gene expression in mammals Use the PEI calculator for planning AAV packaging transfections Find...

  6. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ... commonly used are GECIs - genetically encoded calcium indicators (e.g. GCaMP). Others include: pHluorin... of how bright is the emission. Brightness is calculated as the product of extinction coefficient and ...

  8. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...-55 °C for the homologous region (calculated using Oligo Calculator). Insertions - To insert short sequences...

  9. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...also need to know the extinction coefficient to calculate the concentration. At 280 nm, the extinction coefficient...protein standard and the concentration may be calculated using imaging software. Any bands on the gel ...

  10. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ... ligands (Figure 2). Biosensors, which include calcium and voltage sensors, are the broadest category ... vectors available in the repository. GCaMP, a calcium indicator (biosensor, by Addgene’s categorization...

  11. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ...level of antigen present in the sample and is calculated by extrapolating from the standard curve. Because...amount of antigen present in the sample and is calculated by extrapolating from the standard curve. Scientists...

  12. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...Used for neuronal/CNS expression. Modulated by calcium and calmodulin. GAL1, 10 General expression mRNA...ADH1 General expression mRNA Yeast promoter for alcohol dehydrogenase I Repressed by ethanol Full length...

  13. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    Published
    March 7, 2023, 2:15 p.m.
    ...value cut off. This method entails arranging all calculated p values from smallest to largest and ranking...ranking them (1 being the smallest value). It calculates the cut off for significance by finding the largest...

  14. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ... the Thermostable Ligase Reaction Temperature Calculator can be used to estimate an appropriate incubation...polymorphisms in African samples of Plasmodium falciparum." Journal of clinical microbiology 51.8 (2013...

  15. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...and tween as extended treatment can lyse cells. Alcohols, like ethanol or methanol, fix and permeabilize...first to freeze everything in place followed by alcohol permeabilization. Users should also keep their ...

  16. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...Degorce et al., 2009), measuring intracellular calcium (Miyawaki, Griesbeck, Heim, & Tsien, 1999), monitoring...which 50% FRET occurs. b Range corresponds to calculated D/A distances for observed FRET efficiencies ...

  17. 2021 Deck the Lab Winners!

    Type
    Blog Post
    Published
    Jan. 25, 2022, 2:15 p.m.
    ...December 10, 2021   2nd Place: The Winter and the Falcon Tube created by the team efforts of the Yit Heng...

  18. Deck the Lab 2022!

    Type
    Blog Post
    Published
    Jan. 17, 2023, 2:15 p.m.
    ... have decorated their labs with everything from Falcon tube trees to giant paper mache Krampus performing...

  20. Celebrating One Million Plasmids Shared!

    Type
    Blog Post
    Published
    Sept. 25, 2018, 2:39 p.m.
    ... This massive scale reagent sharing led us to calculate how much time we have saved researchers. In a ...
Showing: 41 - 60 of 120 results