We narrowed to 121 results for: ALC

...will image these neurons using GCaMP7, measuring calcium signaling related fluorescence during morphine ...enable multiplexed imaging of neurotransmitter, calcium, and membrane voltage,” says Tian. Figure...

...the transfection method (i.e. polyethyleneimine, calcium phosphate or liposomal methods), the volume of ...be optimized prior to starting. We recommend calculating the ratio of the surface area of the larger vessel...

...to your normal control - you can set up your calculations so that your assay output number (RQ value) ... determination of amplification of Plasmodium falciparum multidrug resistance-1 gene (pfmdr1). J Parasitol...

...vectors expressing GCaMP6f with this promoter. Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f...terminal providing better signal to noise for imaging calcium dynamics in fine structures. We have expanded our...

...qPCR Protocol for more details. Pro Tips When calculating the titer for your AAV, remember to account for...tool for gene expression in mammals Use the PEI calculator for planning AAV packaging transfections Find...

... plate Fidelity of assembly (percent correct) Calculated colony totals Per 2 μl assembly reaction Per...outgrowth volumes described in Figure 3, with calculated yields from entire outgrowth built from 2 µl ...

... commonly used are GECIs - genetically encoded calcium indicators (e.g. GCaMP). Others include: pHluorin... of how bright is the emission. Brightness is calculated as the product of extinction coefficient and ...

...also need to know the extinction coefficient to calculate the concentration. At 280 nm, the extinction coefficient...protein standard and the concentration may be calculated using imaging software. Any bands on the gel ...

... and ways to navigate it, we’ll search for the calcium biosensor, GCaMP, and click through to the plasmid...distribution. If your experiment involves delivering a calcium biosensor using AAV, you might decide you’d really...

...-55 °C for the homologous region (calculated using Oligo Calculator). Insertions - To insert short sequences...

... ligands (Figure 2). Biosensors, which include calcium and voltage sensors, are the broadest category ... vectors available in the repository. GCaMP, a calcium indicator (biosensor, by Addgene’s categorization...

...level of antigen present in the sample and is calculated by extrapolating from the standard curve. Because...amount of antigen present in the sample and is calculated by extrapolating from the standard curve. Scientists...

...Used for neuronal/CNS expression. Modulated by calcium and calmodulin. GAL1, 10 General expression mRNA...ADH1 General expression mRNA Yeast promoter for alcohol dehydrogenase I Repressed by ethanol Full length...

...value cut off. This method entails arranging all calculated p values from smallest to largest and ranking...ranking them (1 being the smallest value). It calculates the cut off for significance by finding the largest...

... the Thermostable Ligase Reaction Temperature Calculator can be used to estimate an appropriate incubation...polymorphisms in African samples of Plasmodium falciparum." Journal of clinical microbiology 51.8 (2013...

...and tween as extended treatment can lyse cells. Alcohols, like ethanol or methanol, fix and permeabilize...first to freeze everything in place followed by alcohol permeabilization. Users should also keep their ...

...Degorce et al., 2009), measuring intracellular calcium (Miyawaki, Griesbeck, Heim, & Tsien, 1999), monitoring...which 50% FRET occurs. b Range corresponds to calculated D/A distances for observed FRET efficiencies ...

...December 10, 2021   2nd Place: The Winter and the Falcon Tube created by the team efforts of the Yit Heng...

...AAV preparation. For detailed instructions and calculations on how to prepare a plasmid stock used to generate...

... have decorated their labs with everything from Falcon tube trees to giant paper mache Krampus performing...