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We narrowed to 46 results for: Free Use GFP

Showing: 41 - 46 of 46 results

  1. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...mammalian cells. Their marker-free coselection, analogous to methods previously used in C. elegans, targets ...can be used to measure the size of DNA fragments. Depending on which restriction enzyme is used to cut...mutants), gRNAs used for TZAP gene editing, and a Hi6xs-MBP tagged TZAPznf9-11constructs used for bacterial...power of mCyFP1, the Lin and Yasuda groups use both mCyFP1 and EGFP as FRET donors in experiments simultaneously...popular CRISPR base editing technique uses dCas9/Cas9 nickase fused to a cytidine deaminase for targeted...these articles make it easier for you to find and use the plasmids you need. You can find all the hot plasmids...-positive assay) as well as stabilization (Nanog-GFP reporter activation and silencing of factor-linked...

  2. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    Published
    Dec. 10, 2020, 1 p.m.
    ...developed and used AdV vectors to edit genes in several biological systems. It has been used for instance...Addgene and could be useful to labs wishing to create this kind of animal models. Start using adenoviral vectors...Benoit Giquel.   Adenoviral vectors (AdVs) have been used for many years for in vivo delivery and gene therapy...donor DNA is copied into the cut site through error-free homologous recombination (HR). Adapted from an image...viral particle. The earliest explorations of the use of AdV for in vivo CRISPR-Cas delivery utilized the...homology directed repair and you would probably need to use a two viral vectors system: one vector for Cas9/sgRNA...Dystrophy, Alpha1-antitrypsin deficiency (most widely used so far) or for cancer gene therapy such as nonalcoholic...

  3. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...validated your gRNAs for use in mammalian cells, it’s time to use them in mouse embryos. When researchers... Young. The use of CRISPR/Cas9 for gene editing has expanded since its adaptation for use in mammalian...CRISPR Software Matchmaker can be used to determine the pros and cons of using many different types of gRNA...weeks old and use the ear tag method (creating different patterns of ear holes and using that material...and fastest way to use the system in mice? We'll have a post that goes into the mouse genome editing process...efficacy in vitro, and using it in mouse embryos to generate gene modified mouse lines. Hopefully this ... of EGFP to recombine by homology directed repair, and resulting in the expression of EGFP. Using this...

  4. Hot Plasmids: Summer 2025

    Type
    Blog Post
    Published
    Aug. 28, 2025, 1:15 p.m.
    ...tend to photobleach rapidly, which hinders their use for long-term imaging or advanced techniques like...delivery of an RNA-guided genome editor for transgene-free germline editing in Arabidopsis. Nature Plants, ...Recovery (LARRYv2) barcoding libraries. The team used these libraries to analyze pre-existing heterogeneity...tools in your experiment. These libraries will be useful for studying the impacts of other cancer mutations...Addgene has tested these capsid plasmids in vitro and uses them in our own AAV production processes. Check ...Can't wait? Browse our In-Stock AAV inventory or use our Packaged on Request service for thousands of ...eligible AAV plasmids to order viral vectors ready to use in your lab. Find Addgene’s AAV6 and AAV11 packaging...

  5. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ... by using a spreadsheet. The plasmid information can be entered using our online form or by using a downloadable...gRNAs are used for directing CRISPR/Cas9 to a targeted genomic location, while shRNAs are used to knockdown...that can be used under BSL1 conditions were generated in your lab are necessary for use of a plasmid...deposited with us and distribute them to scientists worldwide. What's more, depositing is free! We've written...Either choice can be used for deposits of less than 10 plasmids but we recommend using the spreadsheet option...IDs.   Name – we encourage the use of descriptive plasmid names and using the exact names listed in your...needed. Use the "Relevant mutations/deletions" box to describe any mutations within your insert using standard...

  6. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ... might serve as a starting point for developing useful tools. A lucky start As Andrew and Maria explained...Cohen, A. E. (2011). Mapping Nanomagnetic Fields Using a Radical Pair Reaction. Nano Letters, 11(12), 5367...protein they tested — E. coli expressing good ol’ EGFP — showed a slight but detectable change in fluorescence...better, multiple other labs have reproduced their EGFP findings since the initial report in 2023.)   ...   Figure 1: Fluorescence intensity of EGFP-FlavinTag in E. coli changes in response to a ~25 mT...proteins display a similar effect (many do). Fusing EGFP to the self-labeling FlavinTag made a reliable one-component... the proteins that had shown magnetic responses: EGFP, mScarlet, and AsLOV2. After several rounds of semi-random...
Showing: 41 - 46 of 46 results