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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 262 results for: INO

Showing: 41 - 60 of 262 results

  1. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    Published
    Nov. 6, 2018, 1:12 p.m.
    ... from donors. Apart from fibroblasts, human keratinocytes from hair pluck, peripheral blood cells, and...Chondrocytes [27]         Adipocytes [28] Keratinocytes [29] Photoreceptors [30] Otic hair cells [...of induced pluripotent stem cells from human keratinocytes. Nat Biotechnol, 2008. 26(11): p. 1276-84. PubMed... human induced pluripotent stem cells into a keratinocyte lineage. Methods Mol Biol, 2014. 1195: p. 1-...

  2. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    Published
    June 17, 2014, 7:57 p.m.
    ...domain is interesting because by changing certain amino acids you can change the specificity,” explained...explained Zhanar Abil of the University of Illinois at Urbana-Champaign. The PUF proteins include eight modular...consecutive base in its RNA target. By changing amino acids, it’s possible to produce a protein that binds...

  3. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    Published
    Sept. 28, 2020, noon
    ...and Cas9, which are separately inducible by L-arabinose and anhydrotetracycline (aTet), respectively. ...enzyme cloning. Streptomyces bacteria are more recombinogenic than E. coli, so this system functions more...effectively able to introduce point mutations in P. aeruginosa, Pseudomonas putida, Pseudomonas fluorescens,.../10.1038/s41467-019-11479-0 Peters JM, Koo B-M, Patino R, Heussler GE, Hearne CC, Qu J, Inclan YF, Hawkins...

  4. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ...technology? Let us know.  References Ishino Y, Hideo, S, Makino K, Mitsuko A, Nakata A. J Bacteriol. ...the Type I-F CRISPR/Cas system of Pseudomonas aeruginosa (6). However, these genes only deactivate the...

  5. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ...at all levels.      Jargon is field-specific terminology or language. This can refer both to terms that...that are only used in the field and terminology that is widely used but has a very specific use-case (definition... your career as a scientist underrepresented minorities in your local high school who are also students...

  6. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ... the prediction of T cell epitopes based on the amino acid sequence of the antibody.  NetMHCPan is one...adverse reactions to drugs: adverse reaction terminology. WHO Collaborating Centre for International Drug...Immunogenicity Assessment for Sequences Containing Unnatural Amino Acids: A Method Using Existing in silico Algorithm...

  7. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    Published
    May 3, 2018, 1:48 p.m.
    ...orthologs from Eubacterium siraeum (EsCas13d) and Ruminococcus sp. (RspCas13d), while Konermann et al. characterized...Anaerobic digester metagenome” (AdmCas13d) and Ruminococcus flavefaciens (nicknamed CasRx), as well as EsCas13d...with WYL domains (named for the three conserved amino acids in the domain). Yan et al. from Arbor Biotechnologies...

  8. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...used for bacterial protein purification. When arabinose is absent, regulatory protein AraC binds O and...pBad, blocking transcription. The addition of arabinose causes AraC to bind I1 and I2 sites, allowing ...allowing transcription to begin. In addition to arabinose, cAMP complexed with cAMP activator protein (CAP) can...

  9. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...complex mix using affinity chromatography against an amino acid sequence, or tag, on the protein. While a variety...proteins. Despite being a relatively low frequency amino acid, proteins containing two or more adjacent histidines... The polyhistidine tag is a string of histidine amino acids on the N- or C- terminus of a protein. Neither...

  10. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ...systems such as Cascade-Cas3, Cas9, Cas12, and Cas13 (Marino et al., 2020). Some Acr proteins are specific to...tissues The small size of Acr proteins (~50-200 amino acids) means they can be delivered in vivo using...401 . https://doi.org/10.1016/j.omtm.2018.04.008 Marino ND, Pinilla-Redondo R, Csörgő B, Bondy-Denomy J...

  11. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type
    Blog Post
    Published
    June 26, 2018, 1:33 p.m.
    ...that change protein sequence affect arginine (Arg) amino acids more frequently than any other. Indeed, nearly... that cause human disease change Arg to another amino acid. Clearly, the development of a pharmacological...millions of individuals worldwide.Arg is unique among amino acids in that it possesses a guanidine moiety at...

  12. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...highly specific antibody to the C-terminal five amino acids, meaning the tag could be quite small. However... the epitope recognized by Myc1-9E10 down to 10 amino acids (EQKLISEEDL) - larger than the substance P... et al., who published a paper showing that the amino acids neighboring the Myc epitope influence 9E10...

  13. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...reach zooxanthellae, the coral’s symbiotic dinoflagellates that are photosynthetic (Salih et al., 2000...luminescence, which require UV lamps, fluorometers, or luminometers, chromoprotein detection can be done by the ... the chromoprotein Ultramarine with hydrophilic amino acids to reduce non-specific interactions with cytosolic...

  14. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    Published
    Feb. 25, 2016, 3:30 p.m.
    ... Museum of Indianapolis. I drove passed these dinosaurs every day. They made me happy. Grad school can...made me happy. I started volunteering in the “Dinosphere” almost every week. I gained experience explaining...visitors of all ages, learned a lot about the dinosaurs, and met wonderful people. When I started volunteering...

  15. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ..., showing how DNA sequences are translated into amino acids. And I was astonished that the molecular language...But be careful — antibiotic mechanisms vary! Aminoglycoside antibiotics also block protein synthesis, and...protects against any of them. But different aminoglycosides work on different host species: kanamycin targets...

  16. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    Published
    Sept. 1, 2020, 1:15 p.m.
    ...non-endogenous sequences, such as a 77bp long highly recombinogenic homology sequence derived from the filamentous...used a hybrid split vector with a 872 bp long recombinogenic homology sequence to deliver LacZ to muscle...Garcia-Hoyos M, Gibbs D, Kim SR, Maguire A, Rex TS, Di Vicino U, Cutillo L, Sparrow JR, Williams DS, Bennett ...

  17. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...flexibility in gRNA placement (e.g., mutating a specific amino acid or introducing a specified edit). However, ...mutated to be more specific for exact gRNA matches (Rabinowitz and Offen). Of note, most of these optimized ...CRISPR’ing! References and Resources   References Rabinowitz R, Offen D. Single-Base Resolution: Increasing...

  18. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    Published
    Oct. 8, 2020, 1:15 p.m.
    ...luminescence. This can then be detected by a luminometer. Non-specific DNA stains can be added in the ...Mycoplasma Removal Agents (MRA) are derivatives of the quinolone family of antibiotics and are broad-spectrum antibiotic...culture media. Also, drugs like BM Cyclin, fluoroquinolone ciprofloxacin, ciprobay, zagam, baytril, tetracycline...

  19. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...incorporation of unnatural amino acids (UAAs) into proteins in mammalian cells. Each tRNA/aminoacyl-tRNA synthase ...conjugation reactions, fluorescent amino acids, posttranslationally modified amino acids, photoaffinity probes...  These plasmids are unpublished Unnatural amino acid incorporation in mammalian cells Peter Schultz...

  20. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ... are among the smallest Cas enzymes (400 to 700 amino acids), use a Cas14 enzyme, and uniquely target ...Harrington, L. B., Burstein, D., Chen, J. S., Paez-Espino, D., Ma, E., Witte, I. P., Cofsky, J. C., Kyrpides...Fedorova, I., Karneyeva, K. A., Artamonova, D. N., & Severinov, K. V. (2021). Type III CRISPR-CAS systems: deciphering...
Showing: 41 - 60 of 262 results