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Showing: 41 - 60 of 60 results

  1. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...Lentiviral AmCyan-P2A-mCherry P2A Mammalian pC5Kan-P2A P2A Insect pUltra P2A and T2A Lentiviral ...

  2. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...PubMed Central PMCID: PMC2822740. 2. Engler C, Kandzia R, Marillonnet S. A one pot, one step, precision...

  3. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ... plant selection markers (basta, hygromycin, kananamycin, tunicamycin) (Nakagawa et al., 2007). Alternatively...

  5. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...aminoglycosides work on different host species: kanamycin targets the prokaryotic ribosome, while G418 (...

  6. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...tetracycline repressor TetR and resistance selection marker KanR. This strain is then transformed with the sgRNA ...

  7. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...design in public schools and was started in 2005 in Kansas by Bobby Henderson, a physics graduate from Oregon...

  15. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...https://doi.org/10.1038/nature24049 Altae-Tran, H., Kannan, S., Suberski, A. J., Mears, K. S., Demircioglu...

  16. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ... ideal number. First you generate four unique kanamycin-resistant plasmids, each containing a different...

  19. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...Masayuki, Minoru Iijima, Manami Ohtaka, and Mahito Nakanishi. "Novel Strategy to Control Transgene Expression...
Showing: 41 - 60 of 60 results