...now. When titrating AAV by qPCR, viral DNA is amplified and monitored in real time. When the PCR is complete.... Each droplet contains the ingredients for amplification of the target DNA. This partitioning reduces...droplet contains the material required for a mini amplification reaction to take place.  When the PCR is complete...each one. Droplets that fluoresce contain the amplified target sequence. After all of the droplets have...number of cycles. After additional rounds of amplification, your fluorescence amplitude will increase, ...

...inexpensive to grow and maintain in the lab, have a short life cycle of 8 to 14 days, and produce a large number.... For example, the Stainier lab studied the proliferation and differentiation of β-cells in the the developing...elegans are small, transparent worms that have a rapid life cycle and large brood sizes. The majority of C. ... is that they are transparent throughout their lifetime. Thus any genetic modification, such as expression... al. "Distinct populations of quiescent and proliferative pancreatic β-cells identified by HOTcre mediated...

...scFv sequences have traditionally been cloned by amplifying the VLand VH antibody sequences of hybridomas...for high affinity scFvs. Bound phage are then proliferated by infecting bacteria for additional rounds ... high affinity binding. Bound phage are then proliferated by infecting bacteria for additional rounds ...applications of scFvs: The SunTag system uses an scFv to amplify the fluorescence intensity of a tagged protein.... about the SunTag system which uses an scFv to amplify the fluorescence intensity of a tagged protein....

...CyOFP1, a large stokes shift, long fluorescence lifetime, and a stable monomeric form as determined by ...resonance energy transfer) and FLIM (Fluorescence Lifetime Imaging Microscopy) applications. To demonstrate...isolated and characterized: mScarlet (fluorescent lifetime of 3.9 ns, quantum yield of 0.70), mScarlet-I ...maturation in cells compared to mScarlet, fluorescent lifetime of 3.1 ns, quantum yield of 0.54) and mScarlet-H...photostability compared to mScarlet, fluorescent lifetime of 1.3 ns, quantum yield of 0.20). All three mScarlet...comprised of 2 plasmids, pPSU1 and pPSU2, that can be amplified affordably in bacteria. They are compatible with...

...multiple sites on an antigen, are cheap and can help amplify a weak signal. However, they have high cross-reactivity... smells like rotting eggs but has a longer shelf life when stored properly. Because BME is not as strong...hours Easy and cheap to handcast Short shelf-life; can alter proteins due to high pH Bis-tris ...proteins <40 kDa 180 V, 30 minutes Good shelf life; runs at neutral pH (reduced protein alteration)...proteins > 30 kDA 200 V, 30 minutes Good shelf life; runs at neutral pH (reduced protein alteration)... that work as signal enhancers. These blockers amplify signal without increasing background noise and ...

... whole genome amplification (by a mechanism known as multiple displacement amplification) even in the ...that is refractory to enzymatic manipulation, amplifying phage DNA with phi29 polymerase prior to fragmentation...cloned into E. coli cells, isolated colonies will be lifted onto nitrocellulose membranes and exposed to IPTG...the plate (saving the plate so you can regrow the lifted colonies overnight), flip the membrane over and...

...immunocytochemistry assays. Dewari et al, eLife 2018;7:e35069 DOI: 10.7554/eLife.35069. Encouraged by the high ...Operetta imaging system. Dewari et al, eLife 2018;7:e35069 DOI: 10.7554/eLife.35069. How to set up csRNP knock-in...mammalian stem cells using Cas9 ribonucleoprotein. Elife 7. PubMed PMID: 29638216. PubMed Central PMCID: ...

...scientists are overrepresented on Twitter Mathematical, life, and physical scientists are underrepresented. It...domains between 0-10% of the time; however, ~20% of life scientists  (biologists, neuroscientists, ecologists...follower network is shown. Top: The follower network of life (green), physical (blue) and social (orange)  scientists...subgroups of scientific disciplines: social (left), life (center), and physical (right). How do you use Twitter...

...growth opportunities in life sciences       Propel Careers: Propel Careers is a life science search and career...Immigration officers use to determine if an applicant qualifies for the National Interest Waiver. The three prongs...and no job offer is needed for this category. To qualify, you need to satisfy 3 out of the 10 criteria listed...assist companies and foreign nationals working in the life sciences community obtain their working visas, permanent...

...major research applications. eLife, 12, RP91645. https://doi.org/10.7554/eLife.91645 Biddle, M. S., Alende.../FTD disease gene C9ORF72. eLife, 8, e48363. https://doi.org/10.7554/eLife.48363 Uhlen, M., Bandrowski...of interest is critical to cell survival or proliferation, an additional challenge is that only partial...

...as the spectrum shift occurs during Timer protein life due to a form of oxidation (dehydrogenation). MitoTimers...that you need. The Golden GATEway cloning kit simplifies the cloning process for complex DNA constructs...signaling dynamics are influenced by the relatively long life-cycle of a protein from expression to degradation...the same transgene as the Brainbow cassette, simplifying experiments when additional cross-breeding steps...between the vector and gene primer for SLIC cloning. Amplify the pCoofy vector and the sequence of gene of interest...cassette, which is not copied during the PCR amplification step, so that only vectors with the desired ...

...detected with tyramide signal amplification. Tyramide signal amplification occurs when HRP converts a fluorescently...Tyramide signal amplification of HRP Staining cultured cells and tissue sections Amplifying weak signals...

...are having difficulty amplifying your target sequence, or if you want to amplify sequences from a large...efficient, but if you are having difficulties in amplifying a template, particularly from a large genome,...correct insert Sequence the regions that have been amplified by PCR, putting special attention in the areas...

...and a Senior Research Associate at the Labor and Worklife Program at Harvard Law School. She recently launched...is a Senior Research Associate at the Labor and Worklife Program at Harvard Law School. He has continually...R. "A fair deal for PhD students and postdocs." Elife 2 (2013): e01139. Pubmed PMID: 24137543. Pubmed ...Marder, Eve. "Looking out for future scientists." eLife 3 (2014): e04901. Pubmed PMID: 25291257. Pubmed ...

... of my life, even though that’s what I thought,” she said. “I’m so excited to see where my life will take... as a research and technical assistant, when her life changed––she had a daughter. Needing a career switch...she loves to do, she would never work a day in her life. She said, “it now makes sense because I love working...

...for experimental use. Thus, the first step is to amplify your library to a concentration that is sufficient...next-generation sequencing to check the quality of your amplification. If you've obtained a ready-to-use lentiviral.... Figure 2: The CRISPR library must be amplified (A) to be used to generate lentivirus (B). Cas9... As such, sequencing CRISPR libraries after amplification and after a screen requires the use of next-...

...inclusive. CBE Life Sciences Education. 13(4):602-606. Brian, D. 1996, Einstein: A Life. Chapter 17, Einstein... gains and positive STEM attitudes in CUREs. CBE Life Sciences Education. 21(4):ar74. Duboue, E., Kowalko...chemical biology, biochemistry, and neurobiology. CBE Life Sciences Education. 15(4):ar55. Ma, Y., Mukherjee...

...Expansion Microscopy (TREx). Elife 11:e73775. https://doi.org/10.7554/elife.73775  Dani A, Huang B, Bergan...Expansion microscopy of C. elegans. Elife 9:e46249. https://doi.org/10.7554/elife.46249  Additional resources ...

...yes or no” kind of test, with a positive clone amplifying a product and a negative clone resulting in no...and without an insert to verify that the primers amplify the expected size PCR products.    Primer Design...same backbone plasmid. If this control doesn’t amplify a product, then you know there could be something...insert-specific primers. If your negative control amplifies a product of the expected size, you know the genome...

...beta platform for sharing, annotating, and storing life science protocols. Since we began sharing this protocol...Currently in beta testing, the software aims to be a life science protocol repository. The software can be...have a record of your work. This feature further simplifies lab work if you like to bring your phone in the...and track inventory and orders. Quartzy caters to life science labs and offers the service free of charge...