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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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In Category

We narrowed to 134 results for: Lta;

Showing: 41 - 60 of 134 results

  1. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...of its ability to target multiple gene loci simultaneously, CRISPRa can also be used to map molecular ...nmeth.3871 Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead EH, Guimaraes C, Panning...

  2. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...overnight revelation, rather it was the result of consultations between researchers and publishers. Nonetheless...encouraging authors to integrate datasets with their resultant figures. Instead of providing a “dead end” figure...

  3. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...channelrhodopsins and tools based on light-oxygen-voltage (LOV) domain. They can also be used in crosstalk-free...directed evolution approach applied to fluorescent voltage reporters. Nat Chem Biol 14, 352-360 (2018). Yu...

  4. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ...gel purification. In gel purification, you use a voltage difference across a gel matrix (usually agarose...positioned toward the cathode (- charge). When the voltage is applied across the gel, the DNA migrates toward...

  5. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ... tend to show their limits when it comes to simultaneously modifying multiple loci (multiplexing). Currently...(MAGE) (1). MAGE uses recombineering (2) to simultaneously incorporate multiple single-strand DNA (ssDNA...in a broad range of strains. Thus, pORTMAGE simultaneously allows genome editing and mutant library generation...

  6. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ... With this technique, multiple genes can be simultaneously silenced if a consensus sequence is used (~... tissue for downstream characterization. To simultaneously address both limitations of the traditional...

  7. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...proteins. A type VII is already on the horizon (Altae-Tran et al., 2023)! References and Resources References...), 280–284. https://doi.org/10.1038/nature24049 Altae-Tran, H., Kannan, S., Suberski, A. J., Mears, K....

  8. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...protein that enables imaging of two biosensors simultaneously   Researchers in the labs of Michael Lin ...mCyFP1 and EGFP as FRET donors in experiments simultaneously measuring the activities of CaMKII and RhoA...

  9. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    Published
    April 11, 2023, 4 p.m.
    ...a way to show different aspects of the data simultaneously. That’s why I turned to dashboards. Dashboards...

  11. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    Published
    March 11, 2014, 5:55 p.m.
    ...study shows that the CRISPR/Cas9 system enables simultaneous disruption of two target genes in one step without...

  13. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    Published
    Dec. 31, 2019, 2:26 p.m.
    ... 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins by Cary...

  14. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ...also developed one of these systems further for simultaneous gene activation and editing. Read the paper...

  15. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ...our AAV vector inventory. References Darvesh, Altaf S., et al. "In vivo brain microdialysis: advances...

  19. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...CRISPR-Act3.0, developed by Yiping Qi's lab, allows simultaneous gene activation in plants.  FnCas12a, unlike...
Showing: 41 - 60 of 134 results