We narrowed to 99 results for: MYC

...80S; inhibits chain elongation 100-800 ug/mL Hygromycin B hygB HeLa, NIH3T3, CHO, Jurkat T cells Blocks...80S; inhibits chain elongation. 50-500 ug/mL Puromycin pac HeLa, 293HEK, Jurkat T cells Inhibits protein...do not confuse this with G418 (aka Geneticin). Neomycin should not be used for mammalian expression – ...confers G418 resistance; however, like gentamicin, neomycin is typically used to suppress bacterial growth...

...10.1093/genetics/iyae126.   Gene disruption in Mycobacterium abscessus using a dual-plasmid CRISPR/Cas9 system...dual-plasmid CRISPR/Cas9 system for gene disruption in Mycobacterium abscessus, an increasingly common human pathogen...-based systems have been developed for other mycobacteria, but such systems don’t always work across species...for rapid and efficient genetic disruption in Mycobacterium abscessus. J Bacteriol., 206(3), e0033523. https...

...Gluconacetobacter xylinus, Haemophilus influenzae, Hypomycrobium X, Legionella pneumophila, Methylophilus methyltrophus...calcoaceticus, Actinobacillus pleuropneumoniae, Actinomyces naeslundii, A. viscosus, Aerobacter aerogenes...Methylophilus methylotrophus, Moraxella spp., Mycobacterium aurum, M. smegmatis, Paracoccus denitrificans...spheroides, Salmonella spp., Serratia marcescens, Streptomyces lividans, Synechococcus spp., Thiobacillus ferrooxidans...

...shared by bacteria and eukaryotes. For example, puromycin inhibits protein synthesis across kingdoms by ...targeting a shared aspect of ribosomal function. A puromycin resistance gene on your vector can protect both...antibiotics also block protein synthesis, and the neomycin resistance gene protects against any of them. ...aminoglycosides work on different host species: kanamycin targets the prokaryotic ribosome, while G418 (...

...backbone contains a kanamycin resistance cassette, you would plate the cells on kanamycin-containing agar..... Only cells carrying plasmids that contain a kanamycin resistance cassette will grow. However, often ...

...different selection markers (blasticidin, neomycin, puromycin, hygromycin) and fluorescent protein reporters ...

...Understanding drug resistance mechanisms of Mycobacterium tuberculosis Dr. Patricia Sheen, Universidad...interested in understanding the mechanisms behind Mycobacterium tuberculosis resistance to pyrazinamide (PZA...methods and improved therapies for PZA-resistant Mycobacteria tuberculosis. Investigation of multispecies ...

...but was found to have a point mutation in the puromycin cassette that makes it less efficient in some ...with customizable sgRNA from the U6 promoter and puromycin resistance from the EF-1a promoter. It’s a 3rd...PX459) V2.0. It contains a point mutation in the puromycin cassette that makes it less efficient in some ...

...Nathans, Daniel. "Puromycin inhibition of protein synthesis: incorporation of puromycin into peptide chains...Yarmolinsky, Michael B., and L. Gabriel. "Inhibition by puromycin of amino acid incorporation into protein." Proceedings...

... plant selection markers (basta, hygromycin, kananamycin, tunicamycin) (Nakagawa et al., 2007). Alternatively...

...cells; however, Mycoplasma species do not possess cell walls. As a result, Mycoplasma are intrinsically...fantastic example of intrinsic resistance would be Mycoplasma species’ resistance to beta-lactam antibiotics...

... ideal number. First you generate four unique kanamycin-resistant plasmids, each containing a different...construct, you clone all but one of your gRNAs into spectinomycin-resistant plasmids pX330S-2 to pX330S-(last ...monocot and dicot plants, with a choice of either hygromycin or Basta selection. Yang Lab Single Transcript...

...marker such as puromycin, you can (also) select for the positive colonies by puromycin selection. You ...transfected cells (for example, plasmid with a puromycin resistance cassette, such as PX459). After you...

...uses a selection cassette containing genes for hygromycin resistance and the Roller movement phenotype....young adult worms. Once the worms have laid eggs, hygromycin is added to the plates to select for candidate...

... The strategy relies on several observations: Rapamycin is a membrane-permeable pharmacological agent ...induction heterodimerization of proteins that contain rapamycin-binding domains, such as FKBP and FRB. The cell...membrane protein with a FRB domain (MitoTRAP). Rapamycin binds your recombinant protein (via FKBP) and ...

...stably integrate a reporter gene that contains a puromycin resistance gene followed by the target site (the...genome edit:  A donor plasmid that contains a puromycin resistance gene linked to an in-frame H2B-GFP....

...primarily pertains to ORIs in budding yeast, Saccharomyces cerevisiae; however, we’ve also noted some features...required for the replication of fission yeast, Schizosaccharomyces pombe, vectors at the end.) In a previous...

...- and locus-specific gRNAs Step 4: Select for puromycin resistant cells Step 5: PCR amplify and sequence...cassette into the FBL locus. Upon sequencing of puromycin resistant clones, they found that 80% and 50% ...

...plasmids contain a hygromycin resistance gene as the plant-selectable marker and a kanamycin resistance gene...

...toxic or insoluble proteins Origami2 (DE3)** Streptomycin and Tetracycline Contains highly active thioredoxin... General protein expression  m15 pREP4*, ** Kanamycin (pREP4) Cis-repression of the E. coli T5 promoter...