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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 130 results for: NAP

Showing: 41 - 60 of 130 results

  1. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...400,000 cells were stained with the indicated Synaptotagmin-1 antibodies and corresponding Multi-rAb CoraLite...You can see an example of their work on their synaptotagmin 1 page. The data produced is available on the...to selecting high-performing antibodies for Synaptotagmin-1 (Uniprot ID P21579) for use in western blot...

  3. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    Published
    Dec. 31, 2019, 2:26 p.m.
    ...Academic Job Interview by Erik Snapp Addgene depositor and guest blogger Erik Snapp breaks down how to prepare...

  4. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    Published
    Jan. 17, 2018, 2:57 p.m.
    ...encoding Cre recombinase under the control of the synapsin promoter. It can be used to drive Cre expression...AAV. Because Cre expression is controlled by the synapsin promoter, it and therefore mCherry will only be...

  5. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ...and we’ve been using "plasmid" ever since! From napkins to notebooks Although discovered in the early 1950s...initiated at a Hawaiian deli in 1972. Using a deli napkin for paper, a small group of scientists including...

  6. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    Published
    Sept. 12, 2019, 1 p.m.
    ...controls: pAAV-hSyn-DIO-EGFP (50457-AAV5): The Synapsin promoter directs broad, neuronal expression. AAV...all our GABA sensor AAV inventory here. pAAV.hSynap.iGABASnFR (112159-AAV1) Optogenetics Optogenetics...

  8. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...turnover within a neuron? For my PhD I studied a synaptic protein that is linked to neurodegeneration. The...normal cell function, and, because I was studying a synaptic protein, I wanted to measure it’s levels in its...

  9. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...expression of cytoplasmic-tdTomato and Synaptophysin-GFP. Synapse targeting GCaMP6s from the Rylan Larsen...

  11. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...the presence of a specific protease inhibitor, asunaprevir, the viral protease can no longer self-cleave...components are 1) a SMASh-tagged protein and 2) asunaprevir (small molecule). dTAG The degradation tag (dTAG...

  12. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    Published
    Feb. 25, 2016, 3:30 p.m.
    ... myidp.sciencecareers.org mentioned museums. Indianapolis is home to one of "The world’s biggest and best...children’s museum", The Children's Museum of Indianapolis. I drove passed these dinosaurs every day. They...

  13. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...a sequence manager A sequence manager, such as SnapGene, can be very helpful to confirm primer design....currently investigating the molecular biology of synaptic transmission and plasticity.     Javier García-Nafría...

  14. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...is a scaffolding protein known for its roles in synapse development, transmission, and plasticity and is... is a popular marker of excitatory synapses in assays like IHC. In fact, Anti-PSD-95 [K28/43R] has been...

  15. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    Published
    April 19, 2018, 1:25 p.m.
    ...expertise to our mutual benefit.  Our partnership with SnapGene, for example, allows us to display high-quality... into it. At the same time, this project gives Snapgene great exposure to the community of biological ...

  17. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ... neurons (retrograde transport) or towards the synapse (anterograde transport), which is a possible mechanism... storage, make > 25 μl aliquots in 0.5 ml tubes, snap freeze in liquid nitrogen or a dry ice/ethanol bath...

  20. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    Published
    Dec. 2, 2016, 3:30 p.m.
    ... Why #DecktheLab? Help us spread some cheer by snapping a photo of you and your labmates celebrating the...
Showing: 41 - 60 of 130 results