We narrowed to 89 results for: NOG;

1. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

   Type

   Blog Post

   Published

   June 6, 2019, 12:41 p.m.

   ...tracing the engineered cells in vivo, the non-immunogenic violet-excited GFP variant Vex. To understand...

2. Hot Plasmids - February 2022

   Type

   Blog Post

   Published

   Feb. 22, 2022, 2:15 p.m.

   ...be detected on Western blots via engineered immunoglobulin binding domains.  Santilli, R.T., et al. Sci...

3. Antibodies 101: Secondary Antibodies

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   Blog Post

   Published

   Dec. 21, 2021, 2:15 p.m.

   ... antibody generated in goat against rabbit immunoglobulins. References and Resources More resources on...

4. Antibodies 101: Fab Fragments

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   Blog Post

   Published

   Sept. 5, 2024, 1:15 p.m.

   ...when used in structural studies and reduces immunogenicity when used in vivo.     Of course, the lack ...

5. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

   Type

   Blog Post

   Published

   May 11, 2021, 1:15 p.m.

   ...against mCherry, and secondary antibodies coupled to nanogold particles, that allow a high-spatial resolution...

6. Plasmids 101: Cre-lox

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   Blog Post

   Published

   Jan. 13, 2015, 3:47 p.m.

   ... PMCID: PMC49604. 4. Independent control of immunoglobulin switch recombination at individual switch regions...

7. Hot Plasmids - October 2020

   Type

   Blog Post

   Published

   Oct. 13, 2020, 1:15 p.m.

   ...targeting Cas system that can be used in Drosophila melanogaster. Get the plasmids at Addgene The Cas Hybrid...

8. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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   Blog Post

   Published

   July 23, 2020, 1:20 p.m.

   ... Jiang F, Liu J-J, Bray NL, Rauch BJ, Baik SH, Nogales E, Bondy-Denomy J, Corn JE, Doudna JA (2017) Disabling...

9. Popular Retroviral Vectors and Their Uses in Scientific Research

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   Blog Post

   Published

   July 24, 2018, 12:51 p.m.

   ...stem cells, and developing gene therapies for monogenic diseases. Additionally, retroviral vectors can...

10. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type

    Blog Post

    Published

    March 8, 2017, 3:15 p.m.

    ..., X. laevis (frog), D. rerio (zebrafish), D. melanogaster (fly), C. elegans (nematode), S. cerevisiae ...

11. Corralling and Cornering the Goldilocks Antibody

    Type

    Blog Post

    Published

    Aug. 9, 2022, 1:15 p.m.

    ... than polyclonal antibodies because of their immunoglobin component that can be produced in large “copies...

12. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type

    Blog Post

    Published

    Dec. 14, 2021, 2:15 p.m.

    ...doi.org/10.1016/j.neuron.2013.08.032.  Morita S, Noguchi H, Horii T et al. (2016) Targeted DNA demethylation...

13. CRISPR in the Clinic

    Type

    Blog Post

    Published

    Nov. 2, 2023, 1:15 p.m.

    ...blog post on CRISPR’s potential for correcting monogenetic diseases. Now, almost 10 years later, CRISPR’...

14. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type

    Blog Post

    Published

    July 14, 2016, 2:30 p.m.

    ... for in vivo gene delivery due to their low immunogenicity and range of serotypes allowing preferential...

15. Common Injection Routes in Mice

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    Blog Post

    Published

    Jan. 22, 2025, 9:42 p.m.

    ...used when injecting a cell suspension to create xenograft models. Restraint: back scruffing or mechanical...

16. Pooled Library Amplifications

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    Blog Post

    Published

    May 30, 2024, 1:30 p.m.

    ...For larger libraries that require hundreds of nanograms of DNA, you will likely need to perform several...

17. Of Myc and Men

    Type

    Blog Post

    Published

    Jan. 19, 2023, 2:15 p.m.

    ...with the 78 kd glucose-regulated protein and immunoglobulin heavy chain binding protein. Cell 46:291–300...

18. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type

    Blog Post

    Published

    Oct. 12, 2017, 2:26 p.m.

    ...Toolbox for Genome Editing in Cultured Drosophila melanogaster Cells.” G3 (Bethesda) 6(6) (2016):1777-85. PubMed...

19. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type

    Blog Post

    Published

    May 22, 2015, 4:58 p.m.

    ...Replacement by Compound Autosomes in Drosophila melanogaster. Genetics 74, 461–475 (1973). PubMed. More ...

20. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type

    Blog Post

    Published

    Sept. 6, 2018, 12:02 p.m.

    ... in vivo studies, little is known about the immunogenicity and stability of the Cas9 protein in the host...