Skip to main content
Addgene
Showing: 41 - 60 of 225 results

  1. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ...was recently deposited with Addgene by Dr. Mark Prescott. This system was developed for monitoring and ... Rosado, C., Mijaljica, D., Hatzinisiriou, I., Prescott, M., & Devenish, R. J. (2008). Rosella: A fluorescent...

  3. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ...might want to consult the Cheeky Scientist. Their motto is “We Turn Academics into Confident and Successful...English and Spanish. She is based in Spain. The bottom line – there are lots of people out there who want...

  4. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...between the identical loxP or lox511 sites (Figure 1, bottom). Since only one loxP and lox511 site will remain...Sabatini Wenbiao Chen Liquin Luo Massimo Scanziani Scott Sternson Roger Tsien Naoshige Uchida Ian Wickersham...

  6. Antibodies 101: Flow Compensation

    Type
    Blog Post
    Published
    May 16, 2024, 1:15 p.m.
    ... spillover look like and how do I fix it? When plotting two channels against each other, you should be...single-color APC control, with APC and APC-CY7 plotted against each other. Since it is a single-color ...

  7. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ... R, Turner R, Sellick C, Varley P, Rockberg J, Chotteau V. Small-scale bioreactor supports high density... Schwarz H, Eriksson PO, Eriksson K, Shokri A, Chotteau V. Antibody capture process based on magnetic ...

  8. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...meeting educational goals (Kowalski et al., 2016; Scott, 2021). In fact, completion of Provost’s CURE increased...Academy of Sciences, U.S.A. 117 (25):14077-14083. Scott, K. 2021. Classroom undergraduate research experiences...

  9. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...Plasmid #141386-LV [+M], negative control) were immunoblotted against Anti-SARS-CoV-2 Nucleocapsid Protein...or #211757 (middle panel). Samples were also immunoblotted against vinculin as a loading control (right...

  10. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    Published
    March 12, 2019, 1:37 p.m.
    ...eating one before your chalk talk. Bring your own bottle of water. If possible, write down your short aims...did his postdoctoral fellowship with Jennifer Lippincott-Schwartz at the National Institutes of Health...

  11. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ...quantitative DNA hybridization method (DNA dot blotting) had been used to titrate AAV, but this method...8610.4. Hindson CM, Chevillet JR, Briggs HA, Gallichotte EN, Ruf IK, Hindson BJ, Vessella RL, Tewari M...

  12. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ...caterpillar once, vomit, and never do it again. Cichlids (Scott Juntti) Cichlids are 2,000 fish species that evolved...Animal husbandry of diverse species is a real bottleneck. New gif for #MolluscMonday: #Crepidula atrasolea...

  13. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...more about Western blotting check out our blog post The Basics of Western Immunoblotting.) For mass spectrometry...

  14. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...BL, Ma E, Spinner HB, Baney KLM, Chuck J, Tan D, Knott GJ, Harrington LB, Al-Shayeb B, Wagner A, Brötzmann...10.1016/j.cell.2014.02.001  Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O,...

  15. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...detection system, as is commonly used in western blotting or ELISAs.  A popular method for indirect labeling...host species reactivity. Just like for western blotting, a primary epitope-targeting antibody can originate...

  16. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...quizzically and ask why I didn't just say that? Bottom line is to appreciate that your audience needs ...did his postdoctoral fellowship with Jennifer Lippincott-Schwartz at the National Institutes of Health...

  17. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...antibody-based assays, like an ELISA or Western Blotting, but it allows you to visualize a target of interest... antibody application in The Basics of Western Blotting Resources on Addgene.org Find plasmids encoding...

  18. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ... faculty candidate. Sweaty palms. Heart racing. Cotton mouth.The candidate may find it difficult to answer...region you’ll be working. At this point, your budget bottom line costs may be rather high. That’s normal, because...

  19. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...controls, which we will briefly touch on.  Western blotting When comparing samples in different lanes in a... P. H., et al. “Goals and Practicalities of Immunoblotting and Immunohistochemistry: A Guide for Submission...

  20. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...recently deposited with Addgene by Dr. Mark Prescott. Dr. Prescott and Dr. Rod Devenish (both at Monash University...use. The plasmids were validated by both Western blotting and immunohistochemistry, and are summarized in...
Showing: 41 - 60 of 225 results