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Showing: 41 - 60 of 103 results

  1. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ... a protospacer adjacent motif (PAM). They're used to determine the PAM requirements of Cas enzymes. CRISPR-ARE...

  4. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    Published
    Aug. 4, 2016, 2:30 p.m.
    ...NgAgo First, unlike Cas9, NgAgo does not require a PAM sequence for target recognition, which gives a researcher...multiple sites in a single experiment. Also, the NGG PAM sequence might not always be available at an optimal...

  5. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ... consider following these suggestions: Edit the PAM along with your intended edit. This prevents the ...Anzalone et al., 2019). Figure 4: Editing the PAM prevents the prime editor from re-engaging with DNA...

  6. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...Cas enzymes can continue to cleave DNA if the gRNA/PAM site remains intact. In other words, Cas9 will keep...mutations after an HDR event, ensure the gRNA or PAM site is disrupted in the donor. Choosing a type ...

  9. What's New in CRISPR - May 2019

    Type
    Blog Post
    Published
    May 28, 2019, 12:49 p.m.
    ... Nme2Cas9, that recognizes a simple dinucleotide PAM (N4CC), lending to high target site density (average...

  11. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...often be proximal to a PAM site for DNA cutting to occur. The RNA sequence and PAM site confer specificity...

  12. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    Published
    Jan. 11, 2017, 8:47 p.m.
    ...strange breaks at target sites 5' of the 5'NGG3' PAM sequence as directed by a gRNA. 2A-Puro In this plasmid...

  13. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ...positions along the E. coli MG1655 genome with an NGG PAM motif, averaging 19 targets per gene. The lab has...

  15. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ...these orthologs is key: due to differences in the PAM sequences required by each ortholog, a gRNA designed...

  20. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ... scan along the double-stranded DNA, recognize a PAM sequence, allow the complementary base pairing between...
Showing: 41 - 60 of 103 results