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Showing: 41 - 60 of 186 results

  1. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ...successful outcome. How do I find a coach? Start with referrals. Are there scientists you know who have had a ...offices or professional organizations can provide referrals. There are bad coaches out there, so finding a...

  3. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...into two categories: probes and editors. A probe array may consist of at least two sensor modules targeting...via CRISPR techniques. This tool-kit includes an array of FPs as well as additional useful tags, including...

  4. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ... the (1) binding of calcium, (2) allosteric rearrangements of the sensor, and (3) subsequent change in... with an extra apoCaM-binding motif to prevent aberrant binding of the biosensor to endogenous calmodulin...

  5. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...bright and stable fluorescence of the original (Ivorra-Molla et al., 2023). They also named their variant...: https://doi.org/10.21203/rs.3.rs-2941917/v1.  Ivorra-Molla, E., et al. (2023). A monomeric StayGold ...

  6. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...post was contributed by guest blogger Sean Mac Fhearraigh, co-founder of www.assaygenie.com. No matter ...  Many thanks to our Guest Blogger, Sean Mac Fhearraigh! Seán was a PhD student at University College...

  7. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...-020-00361-z Grimm, D., Kern, A., Pawlita, M., Ferrari, F., Samulski, R., & Kleinschmidt, J. (1999). Titration...https://doi.org/10.1089/hgtb.2013.131 Lock, M., McGorray, S., Auricchio, A., Ayuso, E., Beecham, E. J.,...

  8. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...Pyhtila et al., 2023). Our thanks to Rodolphe Barrangou for his authorial contributions to, and support...References Pyhtila, B., Kasowitz, S., Leeson, R., & Barrangou, R. (2023). The Expanding Dissemination and Distribution...

  10. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...later modified by others to produce Murray’s clear solution.  Murray's clear (11) was mostly used to study...

  12. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...single plasmid containing a multi-spacer CRISPR array. Workflow and technical considerations Making...simple workflow: Clone spacer(s) into the CRISPR array Clone desired DNA cargo into the mini-transposon...

  13. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...contain a deletion in the 3’ LTR that prevents aberrant activation of nearby genes. These safer vectors..., Cavallesco R, Gillet-Legrand B, Caccavelli L, Sgarra R, Maouche-Chrétien L, Bernaudin F, Girot R, Dorazio...

  14. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...Cpf1 multiplexing approach using a single crRNA array to express up to 4 crRNAs. Zetsche et al. also suggest...Gene Editing by CRISPR–Cpf1 Using a Single crRNA Array." Nat Biotechnol. 35.1 (2016): 31-34. PubMed PMID...

  15. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ...makers have moved away from long written proposal narratives. Now they require grant seekers to use online...demand for stronger budgets with clear budget narratives, logic models, established partnerships and collaborations...

  16. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ... the lab. GFP is used in research across a vast array of biological disciplines and scientists employ ...maturation, and fluorescence. Biosensors: A wide array of GFP-based fluorescent biosensors has been designed...

  17. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ..., Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F (2013) Multiplex Genome Engineering...Garneau JE, Dupuis M-È, Villion M, Romero DA, Barrangou R, Boyaval P, Fremaux C, Horvath P, Magadán AH...

  18. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...and portability. This method can be used for an array of applications including clinical diagnostics (...detection of nucleic acids in a mixed population for an array of molecular and clinical diagnostic applications...

  19. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...vesicle-expressing species. The gas vesicle genes from Serratia sp. 39006 produced the highest levels of nonlinear... this gene set bARGSer, for bacterial ARG from Serratia sp. 39006. We next tested bARGSer with several...

  20. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...for the indicated ssAAV5s. Figure adapted from Serrano et al. 2023 under a CC-BY-NC-ND license.  Integrating...  Find microglia-targeting AAV plasmids here! Serrano, C., et al. (2023). Simple and Highly Specific ...
Showing: 41 - 60 of 186 results