We narrowed to 63 results for: SHR

1. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

   Type

   Blog Post

   Published

   March 3, 2020, 2:15 p.m.

   ...encoded voltage indicator Article contributed by Shreya Vedantam Genetically encoded voltage indicators...

2. New Tool for Lineage Tracing: The ClonTracer Library

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   Blog Post

   Published

   Sept. 22, 2015, 2:30 p.m.

   ...protocols for in vivo screening with lentiviral pooled shRNA libraries. Later, Bhang says, “When we developed...

3. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

   Type

   Blog Post

   Published

   March 8, 2017, 3:15 p.m.

   ... Choose from: Encodes one insert, encodes gRNA/shRNA, or empty backbone. Purpose Answer “What does...

4. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

   Type

   Blog Post

   Published

   Aug. 20, 2019, 12:31 p.m.

   ...sequence targeting the gene is cloned into a viral shRNA expression vector and the vector particles are delivered...

5. Progress Towards a PAM-Free CRISPR

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   Blog Post

   Published

   March 7, 2024, 2:39 p.m.

   ...location or efficiency. If you are just trying to shred a long non-coding RNA, cutting most anywhere within...

6. CRISPR in the Clinic

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   Blog Post

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   Nov. 2, 2023, 1:15 p.m.

   ...currently in clinical trials includes Cas3 (the shredder member of the Cas family) as part of a cocktail...

7. Viral Vectors 101: Virus Safety

   Type

   Blog Post

   Published

   June 22, 2023, 1:15 p.m.

   ... GFP. Similarly, if the viral vector carries an shRNA to a gene essential for cellular function, this ...

8. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

   Type

   Blog Post

   Published

   Dec. 3, 2019, 11:38 a.m.

   ...matching your search terms with gene and guide RNA / shRNA inserts Resistance Marker: This indicates the selectable...

9. Your Lentiviral Plasmid FAQs Answered

   Type

   Blog Post

   Published

   April 23, 2014, 1:08 p.m.

   ...variety of applications including cDNA expression, shRNA-mediated knockdown, Tet and Cre-regulated expression...

10. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type

    Blog Post

    Published

    Sept. 13, 2018, 1:55 p.m.

    ...identified essential genes while previous Cas9 and shRNA library screens only identified  60% of the essential...

11. Deciphering the Mysteries of Behavior with Viral Vectors

    Type

    Blog Post

    Published

    Sept. 17, 2019, 12:40 p.m.

    ...paper, the authors used lentivirus to introduce an shRNA to downregulate expression of miR124a, a regulatory...

12. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type

    Blog Post

    Published

    May 2, 2019, 12:41 p.m.

    ... less well known fungi (they are not yeast or mushrooms) but there are over 1,000 species. No transformation...

13. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type

    Blog Post

    Published

    Sept. 16, 2020, 1 p.m.

    ...Cas9 must be made smaller. Previous attempts to “shrink” Cas9 include the use of St1Cas9 (~3.3 kb) from...

14. New CRISPR Tools: Cas7-11 and PASTE

    Type

    Blog Post

    Published

    Feb. 17, 2022, 2 p.m.

    ... were reported as up to 85%, broadly similar to shRNA and Cas13 systems in a range of mammalian cell lines...

15. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type

    Blog Post

    Published

    May 13, 2025, 1:15 p.m.

    ...., 2008). 2. Transient expression of transgenes/shRNAs If you want your cells to express a protein or ...

16. CRISPR 101: Homology Directed Repair

    Type

    Blog Post

    Published

    Jan. 26, 2023, 2:15 p.m.

    ...DNA double-strand break repair pathway choice. Shrivastav M, et al Cell Research (2008). PubMed. Mechanisms...

17. A Control for All Seasons

    Type

    Blog Post

    Published

    Oct. 5, 2023, 1:15 p.m.

    ...substituting with knockdown approaches using siRNA or shRNA. If this alternative is not possible due to a lack...

18. Plasmids 101: Restriction Cloning

    Type

    Blog Post

    Published

    Feb. 18, 2016, 3:42 p.m.

    ...choose. CIP (calf alkaline phosphatase) or SAP (shrimp alkaline phosphatase) are commonly used. Follow...

19. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type

    Blog Post

    Published

    Nov. 6, 2015, 3:30 p.m.

    ...always significant. Doubts, fears, insecurities… all shrink when shared with a mentor who has experienced them...

20. Important Considerations When Using AAVs

    Type

    Blog Post

    Published

    June 13, 2017, 2:30 p.m.

    ...such as microRNAs (miRNAs), short hairpin RNAs (shRNAs), antisense oligonucleotides (ASOs), zinc finger...