We narrowed to 151 results for: SPEN;
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TypeBlog PostPublishedOct. 25, 2018, 1:23 p.m....agrobacterial cultures are ready, spin them down and resuspend them in transformation media. If you are dipping...transformant. I like to spin down 100 mL of culture and resuspend in 25 mL of transformation medium per Agrobacterium...
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Plasmids 101: Colony PCR
TypeBlog PostPublishedMay 12, 2016, 2:30 p.m....day, you can save the leftover bacteria-water suspension and use them to start cultures of your positive...up PCR reactions: The remaining bacteria-water suspension will serve as the template for your PCR reaction... -
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog PostPublishedOct. 1, 2015, 2:30 p.m....circuit is reinforcing, the animal will prefer to spend most of its time in the chamber in which it receives...conversely, if the circuit is aversive, it will spend most of its times in the opposite chamber) [2]. ... -
Lab to Office Culture Shock
TypeBlog PostPublishedFeb. 5, 2019, 1:04 p.m....think about how much of that time you’re actually spending doing work. Yes, you may legitimately need to ...interact with...non-scientists! Maybe you’ve just spent 5-10 years learning to “speak science,” and virtually... -
10 Steps to a Perfect Science Talk
TypeBlog PostPublishedAug. 23, 2016, 2:30 p.m.... below “Tell a story”) and note how they build suspense. Notice the simplicity of their slides and how..., you just need to figure out how to make it suspenseful. 4. Start preparing your talk early - like today... -
Technique: Probe Phage Genomes for Host Binding Proteins
TypeBlog PostPublishedSept. 29, 2016, 2:30 p.m.... your membranes with PBST to remove salt, then suspend a freshly harvested dose of cells (preferably in...enjoys pondering how phages get through the day, spending time with her horse, and wondering what her cat... -
Is this the right place for me? 8 tactics for choosing a lab
TypeBlog PostPublishedOct. 2, 2018, 12:56 p.m....logic - even choosing a training lab where we will spend the next 6 years. Know yourself I rotated in 3... lab if at all possible. If not, see if you can spend a day or two hanging out around the lab and especially... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog PostPublishedJune 24, 2020, 5:45 p.m....Lysine-specific Demethylase 1 (LSD1) Tatjana Sauka-Spengler's lab has deposited pX330a dCas9-LSD1 for targeted...Artibani M, Taylor G, Wells D, Ahmed AA, Sauka-Spengler T (2017). Genome and epigenome engineering CRISPR... -
9 tips for a successful postdoctoral experience
TypeBlog PostPublishedOct. 9, 2018, 12:21 p.m....depending on the revisions and the amount of time spent shopping a manuscript. Manuscript writing can take...fit into a future manuscript? You could easily spend a day or a week planning an experiment, even a relatively... -
Addgene's 20th Anniversary Party
TypeBlog PostPublishedJune 11, 2024, 1:15 p.m....committee. However, the majority of the time was spent with Addgenies meeting each other's friends and ... -
Participate in the Pets of Addgene Scavenger Hunt
TypeBlog PostPublishedApril 1, 2021, 1:15 p.m....enough space at HQ and for some of us, that means spending more time with our pets. These new “co-workers... -
#Deckthelab Contest Winners 2017
TypeBlog PostPublishedJan. 31, 2018, 2:44 p.m....was so moved by our deck the lab contest that she spent a year hoarding used tip boxes for a lab supply ... -
Congrats to the 2019 Deck The Lab Winners
TypeBlog PostPublishedJan. 17, 2020, 3 p.m....unseasonably warm weather we had last weekend). We’ve spent the last week perusing the Deck The Lab contest ... -
The Codon Guide to Synthetic Biology
TypeBlog PostPublishedSept. 14, 2023, 1:15 p.m....I spent about seven years working in research labs, and then pivoted to writing full-time in mid-2020... -
Deck the Lab 2022!
TypeBlog PostPublishedJan. 17, 2023, 2:15 p.m....Heng Chooi (@YitHengChooi) December 4, 2021 I spent a year hoarding used tip boxes for a lab supply ... -
Activate Addgene's "Find Plasmids" Badge on Pubmed Abstracts
TypeBlog PostPublishedMay 12, 2017, 12:58 p.m.... an easy way to find published plasmids? Do you spend a lot of time in PubMed searching for research articles... -
Our New Antibody Mascot is....
TypeBlog PostPublishedFeb. 24, 2022, 2:15 p.m....from Affie to Yncredibody. After an agonizing week spent in cloistered consideration, and with much debate... -
Streaking for Single Colonies: The Streak Plate Challenge
TypeBlog PostPublishedMarch 2, 2021, 2:15 p.m....challenging, potentially by using a thicker bacterial suspension,” says Westenberg. In addition to streaking for... -
Kwabena Duedu on Public Health, Biofuels, and Doing Science in Ghana
TypeBlog PostPublishedDec. 20, 2016, 3:30 p.m....’s Newsletter and recently visited Cambridge to spend some time learning and doing research at Novartis... -
Addgene’s Newest Way to Speed Your Research: The Cloning Grade DNA Service
TypeBlog PostPublishedJuly 28, 2020, 1:15 p.m.... what you want to do with your plasmid without spending time and resources in preparative experiments....