CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
Type
Blog Post
Published
Sept. 28, 2020, noon
...targeted repression of gene expression. They successfully knock down expression of essential and non essential... of Gene Expression. Cell 152:1173–1183 . https://doi.org/10.1016/j.cell.2013.02.022
Ronda C, Pedersen...) technology (Ronda et al., 2016). pMA7CR_2.0 expresses lambda Red and Cas9, which are separately inducible...function in bacteria, most efforts to regulate gene expression were limited to inducible promoters or direct... much more user-friendly way to modulate gene expression. The Marraffini lab and Qi lab developed early...repression. Using a Tet responsive promoter to drive expression of a catalytically dead Cas9 enzyme allows reversible...the gRNA cassette, one can reliably knockdown expression of multiple target transcripts simultaneously...