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We narrowed to 215 results for: crispr cas9 plasmids

Showing: 41 - 60 of 215 results

  1. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...for us, CRISPR is an incredibly versatile system that can be tweaked to target ncRNA. CRISPR/Cas9 techniques...1A-C). Find CRISPR plasmids at Addgene! Knock-in: Introducing tags or stop signals Once Cas9 generates ...2021.    Alternative CRISPR systems for targeting ncRNAs While CRISPR/Cas9 has proven to be an extremely...If the CRISPR/Cas9 system is not effective for your experiments, there are plenty of other CRISPR-based...Resources on addgene.org Addgene's CRISPR Guide Addgene's CRISPR 101 eBook CRISPR resources More resources on... DNA, CRISPR interference (CRISPRi) and CRISPR activation (CRISPRa) are powerful tools. CRISPRi uses a... protein-coding genes thanks to systems like CRISPR/Cas9 that allow them to manipulate full genes and ...

  2. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ... Check out our CRISPR Guide Find Plasmids for Cas9 Purification Find CRISPR Plasmids for Mammalian Expression...editing. How to use Addgene plasmids for Easi-CRISPR You can use Addgene plasmids to create a repair template...how you can make CRISPR mouse model generation easi-er! Generating mice before CRISPR The process of generating...generating knock-in mice pre-CRISPR was very arduous. First, you’d construct a plasmid with your desired changes... process takes ~8-10 months. With CRISPR (B), you can inject CRISPR components directly into a zygote ...template and CRISPR machinery, Quadros et al. recommend ribonucleoprotein (RNP) complexes of Cas9 protein ...crRNA and tracrRNA) for maximum Easi-CRISPR efficiency. Easi-CRISPR is compatible with standard microinjection...

  3. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type
    Blog Post
    Published
    July 28, 2015, 2:30 p.m.
    ...with us! Find the Plasmids: Plasmids for protein tagging with CRISPR Additional CRISPR Posts from the ...and efficiently, they’ve recently adapted the CRISPR/Cas9 system for protein tagging. We spoke with Mendenhall...to www.addgene.org/crispr/tagging. Addgene: What motivated you to adapt CRISPR for use in protein tagging.... In adapting the CRISPR/Cas system, what we are doing is adding in a second plasmid to target an epitope...context? Mendenhall: The benefits are two-fold: CRISPR/Cas9 offers high efficiency of inducing double strand...of the second plasmid can be done in one day in one assembly reaction. This second plasmid saves you a ...alerts when plasmids become available.  We’ve also had some requests for variations on the plasmids we’ve developed...

  4. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ... Find CRISPR plasmids by function Find recently deposited plasmids Topics: CRISPR, Other CRISPR Tools... of “What’s New in CRISPR” - where we highlight a few of the newest CRISPR plasmids available at Addgene... Miniature CRISPR-Cas systems SEND plasmids Prime editing improvements Miniature CRISPR-Cas systems ... enzyme with a fully active Cas9 enzyme. PE-Cas9 comprises an active Cas9 fused to the reverse transcriptase... our CRISPR 101 blog posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide... read more content related to CRISPR technology, subscribe to our CRISPR blog content.   In this post ...One drawback to the commonly used S. pyogenes CRISPR/Cas9 system is that it is too large to be effectively...

  5. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ...hybridization (FISH) or fluorescently labeled TALEs, CRISPR/Cas9’s ease of use and ability to label live cells... of chromatin organization. This fluorescent CRISPR/Cas9-based method is a great step forward in live .... As this study joins the pantheon of useful CRISPR/Cas9 techniques, we at Addgene are excited to see ... crispr/cas9... a colorful Cas9 toolbox, Ma et al. turned to SpCas9 and its orthologs NmCas9 and St1Cas9. Each ortholog...states. dCas9 gets a colorful makeover Even without its nuclease activity, catalytically dead Cas9 (dCas9...It seems that there’s a new CRISPR advance or technique published every week! One of the newest applications...

  6. CRISPR Guide Update

    Type
    Blog Post
    Published
    Oct. 10, 2024, 1:15 p.m.
    ...updated CRISPR Guide! The CRISPR Guide helps scientists understand how to use CRISPR plasmids in the ... PRIME Editing CRISPR 101: Cas9 vs the Other Cas(s) Progress Towards a PAM-free CRISPR...engineered Cas9s with more flexible requirements and enhanced editing, as well as alternatives to Cas9. All ...great introduction to what CRISPR technologies have to offer. Happy CRISPR-ing! More resources on the...sections to share and describe some incredible new CRISPR technologies developed and widely adopted in the... as refreshing the existing content. Access the CRISPR Guide here! Highlights include a much-anticipated...prime editing section and additions describing CRISPR’s ability to make large genome edits using transposases...

  7. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...to increase CRISPR editing efficiency across multiple CRISPR enzymes. Plasmids for CRISPR coselection ...podcast segment on CRISPR tools for plants CRISPR inhibitors Bacteria utilize CRISPR Cas9 systems to defend...Ouabain New CRISPR base editors The popular CRISPR base editing technique uses dCas9/Cas9 nickase fused...modification. Plasmid ID Plasmid Name Cas9 Variant Speacial Features 85169  pJL-SaBE3  SaCas9 (NNGRRT)   ...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These brief articles...articles provide a synopsis of a plasmid or group of plasmids' functions and applications. We hope that... you to find and use the plasmids you need. You can find all the hot plasmids from 2017 below. With over...

  8. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...Staphylococcus aureus Cas9 (Sa-Cas) and deposited plasmid IF311: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-LSD1. LSD1 ...types. They have also fused LSD1 to x-Cas9(3.7). x-Cas9(3.7) is a SpCas9 mutant that exhibits increased PAM...deacetylases. CRISPR and epigenetics As with many TALEN-based technologies, the advent of CRISPR has made .... For an up-to-date list of CRISPR epigenetic tools, check out our CRISPR Epigenetics resource. Transcriptional...potential advantage of CRISPR epigenetic tools is their persistence and inheritance. CRISPR activators and repressors...TE, Gersbach CA (2015) Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from ...IB, Crawford GE, Reddy TE, Gersbach CA (2017) CRISPR–Cas9 epigenome editing enables high-throughput screening...

  9. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...Resources on Addgene.org CRISPR Plasmids and Resources CRISPR 101 eBook CRISPR History and Development ...involving CRISPR Cas9 for these diseases was sponsored by Vertex Pharmaceuticals and CRISPR Therapeutics...initiated a CRISPR therapy for hATTR with the first-ever systemic CRISPR therapy. They engineered a CRISPR-Cas...the Addgene blog CRISPR Antimicrobials Treating Muscular Dystrophy with CRISPR CRISPR 101: Drug Discovery...There can be no doubt that CRISPR/Cas9 technology has been a breakthrough for the genome-editing field...post on CRISPR’s potential for correcting monogenetic diseases. Now, almost 10 years later, CRISPR’s potential...and editing strategies for CRISPR therapies. Systemic therapies involve CRISPR-Cas delivery directly to ...

  10. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    Published
    March 19, 2019, 1:31 p.m.
    ...control of CRISPR-Cas9 by CASANOVA Article contributed by Cary Valley  Listen to the anti-CRISPR podcast ...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need... recent plasmid deposit please sign up here.          Listen to this episode of Hot Plasmids! Optogenetic...podcast segment Anti-CRISPR (Acr) proteins potently inhibit type II CRISPR systems including the popular S. ...domains, they have developed a system to control CRISPR-Cas9 activity using blue light (Bubeck et al., 2018...thus, inhibiting function of Acr. This allows CRISPR-Cas9 to function on its target.  They name their most...

  11. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...other CRISPR plasmids are from the Zhang lab. In fact, the Zhang lab has shared over 40,000 CRISPR-Cas9...applications. This plasmid was the top requested plasmid of 2017. Find pMD2.G. pSpCas9(BB)-2A-GFP (PX458...and a gRNA. But instead of Cas9, this plasmid expresses Cas9n (a D10A nickase mutant) to nick, rather than...requested plasmids over the last 15 years - the majority of these being lentiviral or CRISPR tools. How...and an envelope expressing plasmid, such as our second most requested plasmid of all time, pMD2.G. Find...PX458) - This plasmid from the Zhang lab expresses Cas9 from Streptococcus pyogenes with an invariant sgRNA...deliver Cas9 and your gRNA of interest into mammalian cells. This vector was the top requested plasmid of ...

  12. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ... Overview of the dual-plasmid CRISPR/Cas9 workflow in M. abscessus. A) Sth1Cas9 mediates double strand... in Mycobacterium abscessus using a dual-plasmid CRISPR/Cas9 system By Aryana Khosravani Deborah Hung’...Hung’s lab has developed a novel dual-plasmid CRISPR/Cas9 system for gene disruption in Mycobacterium abscessus...first use of CRISPR/Cas9 in M. abscessus. The simplicity and efficiency of this dual-plasmid approach makes..., 2024 under CC-BY license.   Find plasmids for CRISPR/Cas9 in M. abscessus here! Neo D, Clatworthy...Clatworthy A, and Hung, D. (2024). A dual-plasmid CRISPR/Cas9-based method for rapid and efficient genetic disruption...of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.  ...

  13. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    Published
    Jan. 16, 2024, 6:13 p.m.
    ...Editing CRISPR Cheat Sheat Resources from Addgene.org CRISPR Guide CRISPR: Protocol for Genome Deletions in... CHIME works through transduction of Cas9-expressing (Rosa26-Cas9) hematopoietic stem cells with a lentiviral...., Haining, W. N., & Sharpe, A. H. (2019). A CRISPR-Cas9 delivery system for in vivo screening of genes... Addgene blog CRISPR101: Validating Your Genome Edit How to Design Your gRNA for CRISPR Genome Editing...time-consuming, as it only changes one gene at a time. CRISPR-based systems enable gene knockout in immune cells...and in combination with different genes. Having CRISPR systems for assessing genes in defined contexts...Table 1). The four systems Name Function Plasmid Transduction Marker Mouse Strain C-CHIME...

  14. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ...combine CRISPR and AAVs. 9. CRISPR 101: Validating Your Genome Edit Once you’ve used CRISPR/Cas9 to make...: A New Tool for CRISPR Genome Engineering While the vast majority of CRISPR plasmids in our repository...validation process for CRISPR mediated genome engineering. 10. Plasmids 101: Control Plasmids Need help thinking...eBook Download the Plasmids 101 3rd Ed. eBook Read the Most Recent Plasmids 101, CRISPR 101, and Career ...popular posts continue to be those from our Plasmids 101 and CRISPR 101 series. These posts aim to give researchers... they need to get started using plasmids and the revolutionary CRISPR technology in their own labs. We...repair (HDR) and how it’s used in CRISPR genome editing.   2. Plasmids 101: Cre-lox Cre recombinase can...

  15. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    Published
    Jan. 11, 2017, 8:47 p.m.
    ...Deliver CRISPR Plasmids to Mammalian Cells Resources on the Addgene Website Visit our CRISPR Guide Pages...the top requested plasmid from 2016 is an expression vector for CRISPR nuclease, SpCas9 from the Zhang Lab...of mammalian cell lines. Cas9, of course, is the key component of most CRISPR systems and generates double...F. Ann, et al. "Genome engineering using the CRISPR-Cas9 system." Nature protocols 8.11 (2013): 2281-2308...on the Addgene Blog Browse Our CRISPR Posts Learn about Using CRISPR to Generate Knockouts Read Some ...express both SpCas9 and your gRNA in a variety of mammalian cell types. Purpose  SpCas9 This plasmid expresses...sure, as is obvious from our most requested plasmid, SpCas9 is still going strong as the basis for many...

  16. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...Wang X (2015) WU-CRISPR: characteristics of functional guide RNAs for the CRISPR/Cas9 system. Genome Biol...Best Cas9 Variant for Your Next Experiment Find out More about Genome-wide Screens with CRISPR/Cas9 Learn...Learn How CRISPR Has Been Used with AAV Resources on Addgene.org Find Cas9 Activators Find Cas9 Repressors...required for Cas9-based activation. CRISPRa for genome-wide studies One of the best uses for Cas9 activators...Institute for Biologically Inspired Engineering. CRISPR/Cas9 is an enormously plastic tool and has taken the...context of the gene, Cas9 activators efficiently solve these problems.   Which Cas9 activator should I ...Prioritization of cancer therapeutic targets using CRISPR–Cas9 screens. Nature 568:511–516 . https://doi.org...

  17. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ...Addgene.org Read our CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid...blog Download the CRISPR 101 eBook Find our CRISPR 101 blog posts Read our CRISPR cheat sheet Resources... lower average Cas9-independent off-target DNA editing, ~5–50-fold lower levels of Cas9-dependent off-... paper in Scientific Reports Find the plasmids Enhanced CRISPR-based DNA demethylation Figure...Nature Communications Find Casilio-ME plasmids If you have a new CRISPR tool you’ve recently deposited to...editor plasmids CRISPRi in Caulobacter crescentus Figure 1: Serial dilutions of CRISPRi in Caulobacter...

  18. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    Published
    March 21, 2019, 12:47 p.m.
    ...anti-CRISPR expression plasmids CRISPR-based method for cell lineage tracing Valcyrie is a CRISPR-based...Addgene.org Read our CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid...blog Download the CRISPR 101 eBook Find our CRISPR 101 blog posts Read our CRISPR cheat sheet Resources...series will help you find new CRISPR tools for your research.     CRISPR-Cas12b for human genome editing...Communications Find the Cas12b plasmids  Plant base editing with a Cas9-adenosine deaminase fusion Caixia... Development Find the Valcyrie plasmids If you have a new CRISPR tool you’ve recently deposited to...

  19. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ...rough approximations of the total number of CRISPR plasmids for that particular organism available at Addgene...ready-to-use CRISPR lentiviral preps to researchers across the globe. Why provide CRISPR as virus? “Like...receive transfer plasmid DNA that you can use along with lentiviral packaging plasmids to create your own... size of the SpCas9 nuclease (~4.2 kb) this is a particularly prominent problem in CRISPR genome editing...Institute to provide lentiviruses for delivery of SpCas9 and Cas9 nickase for genome editing. You can also enhance...total with the indicated function. One huge reason CRISPR has become such a popular genome editing tool is... is its developers’ willingness to make their CRISPR technologies available to the academic research community...

  20. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ... blog CRISPR 101: Cas9 vs. The Other Cas(s) CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems...methods rely on Cas9 or a derivative of Cas9, but the ever-expanding repertoire of CRISPR has brought many...Using CRISPR-Transposons Additional resources on Addgene.org CRISPR guide Cascade-Cas3 Plasmid collection...Cascade-Cas3 plasmids in Addgene's collection! A “complex” system Cas3 is part of the Class 1 CRISPR family,..., these types of CRISPR systems work using a multi-Cas complex called Cascade (CRISPR-associated complex...mechanical work for editing. Class 1 CRISPR systems make up the majority of CRISPR-based immune systems in bacteria...by anti-CRISPR proteins or running into other proteins naturally bound to the DNA. Cas3 or Cas9? Large ...
Showing: 41 - 60 of 215 results