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We narrowed to 109 results for: crispr library

Showing: 41 - 60 of 109 results

  1. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...Addgene blog: Browse CRISPR articles from our CRISPR topic overview Find other CRISPR methods for bacteria...integration using CRISPR-associated transposases” or MUCICAT. Here, we’ll describe each of these CRISPR systems....N-acetylglucosamine strain library construction in 8 days. To build an equivalent strain library, our pEcCas/pEcgRNA... Resources on Addgene.org: Visit the CRISPR Guide Get CRISPR plasmids from Addgene ...Yang lab, we’re continually optimizing the use of CRISPR editing systems in bacteria. Our pCas/pTargetF ...editing), our MUCICAT system which is based on the CRISPR-associated transposase system, INTEGRATE, is best...N-acetylglucosamine biosynthesis, we constructed a strain library with 1-11 copies of integrated genes. We obtained...

  2. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ...functional score based on its library frequency before selection compared to its library frequency after selection...mutational scanning libraries. While each of these methods works well to generate libraries, they all have ...in Your Plasmids Learn How You Can Use CRISPR Pooled Libraries to Generate Mutant Populations Use REPLACER... Use CRISPR Pooled Libraries to Generate Mutants ...technique relies on the generation of a plasmid library that expresses all desired variants of a protein... this process is the ability to generate full libraries of mutant proteins. Researchers from the Whitehead...technique that can be used to generate complex libraries of mutant plasmids ready for deep mutational scanning...

  3. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...Check out our CRISPR Guide Find CRISPR Plasmids for Mammalian Systems Learn about CRISPR History ...In order to bind DNA, Cas9 and other CRISPR enzymes require a short PAM sequence adjacent to the targeted...encoding phage gene III. The accessory plasmid library contained all 64 possible NNN PAM sequences adjacent...to a protospacer. If ω-dCas9 could recognize a library PAM, it would induce transcription of gene III,...final round of evolution, they used a no-G PAM library to enrich for Cas9 variants with activity on non-NGG...cleavage, they tested the clones against an NNN PAM library in bacteria where cleavage leads to the loss of...Learn about Engineered Cas9 PAM Variants Read about CRISPR Base Editing Learn about Homology Directed Repair...

  4. Hot Plasmids: Summer 2025

    Type
    Blog Post
    Published
    Aug. 28, 2025, 1:15 p.m.
    ...Addgene has pooled libraries for any summer project. While many barcoding libraries are available for ...RNA Recovery (LARRYv2) barcoding libraries. The team used these libraries to analyze pre-existing heterogeneity... TnpB for plant genome editing LARRYv2 pooled libraries for cell barcoding Addgene’s own AAV6 and AAV11... TnpB, so hot right now By Alyssa Shepard While CRISPR has become a household name, the search for smaller...more in our recent blog post on TnpB). Similar to CRISPR-Cas, TnpB is directed to genomic targets using ...9.   Make a splash with new LARRY barcoding libraries By Mike Lacy Whether you’re planning on lounging...(Figure 3). Figure 3: Using LARRYv2 libraries for state-fate analysis in ex vivo HSC expansion...

  5. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...article: CRISPR-based editing in organoid Biosensors for detecting strigolactones The CRISPR Corner New...10.1021/acssynbio.0c00192   The CRISPR corner By Jennifer Tsang New CRISPR plasmids are always being added...To find all of the CRISPR plasmids available from Addgene, head over to our CRISPR Plasmids and Resources...from the past couple months: A new lentiviral CRISPR library from Alec Kimmelman targets mouse metabolic...relative to wild type. A new genome-wide CRISPR knockout library from Xiaole Shirley Liu's lab targets all...system, showing the workflow for inducible CRISPR inhibition (CRISPRi). A) Lentiviral vector design. B) Strategy...New ready-to-use viral preps New toolkit for CRISPR-based genome editing in organoids By Ashley Waldron...

  6. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    Published
    May 13, 2019, 12:01 p.m.
    ...too! The CRISPR field is always changing so we’ve been busy updating our CRISPR guide. Our CRISPR guide ...for planning your CRISPR experiment. For a quick guide to CRISPR, download our CRISPR Cheat Sheet....you use CRISPR. Hope you will join us this week at #AddgeneCRISPRweek. We updated the CRISPR guide! If..., we received the first CRISPR plasmid deposit. Now we have over 8,000 CRISPR plasmids in the repository...That’s a lot of CRISPR! As we’ve shared these tools over the years, the amount of CRISPR educational resources...about CRISPR. We’ll have a different theme for each day. Beginning today, we’ll start with a CRISPR overview...using CRISPR and Wednesday will highlight some Cas9 variants. Next, we’ll look at various CRISPR applications...

  7. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    Published
    March 22, 2016, 2:30 p.m.
    ...How the Clon Tracer Library Has Been Used in Cancer Lineage Tracing Read How CRISPR Can Be Used in Genome-Wide...studies in the literature to curate the perfect library, obtained the appropriate cDNAs from other researchers...and attached a DNA barcode to each to make the library usable for pooled screens. Applications of the ... over the world who are interested to use our libraries to do things - sometimes drug screens and sometimes...possible.” The researchers have plans to expand the library to include activators for more cancer-related pathways...Find Other Kits for Your Research Find Pooled Libraries for Your Research View our Hot Plasmid Pages to...

  8. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...Biology Learn about CRISPR Pooled Libraries Resources at Addgene.org Find pooled libraries at Addgene Find...advancement easier. NEBNext Ultra FS DNA Library Prep Kit To create a DNA library, one must first fragment a DNA...NEBNext Ultra II DNA Library Prep Kit for Illumina, one was only able to extract libraries from previously ...by trying out NEB’s new NEBNext Ultra II FS DNA Library Prep Kit for Illumina sequencing and took a class...previously fragmented DNA.The general process for library preparation using this kit is: Fragmentation and end...sizes and also produced high yields of quality libraries from small amounts of DNA i.e 100 pg. At the start...the end of the workshop, all our newly created libraries were analysed to see how well the kit worked. ...

  9. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...you’re getting started with them. CRISPR Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening...how you can use CombiGEM to barcode small libraries of CRISPR gRNAs to use for screening experiments. Get...Optimizing Donor DNA for Enhanced CRISPR Genome Editing CRISPR has revolutionized how researchers make ... Get the full details on how to make these libraries from the post and the associated publication. Optimizing...and other types of genome edits, but, even with CRISPR, it isn’t always easy to insert a section of DNA...biophysics! Casilio: An Adaptive, Multitasking “CRISPR-OS” In this post, Albert Cheng and Mark Wanner ...

  10. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    Published
    Aug. 13, 2015, 2:30 p.m.
    ...Working with CRISPR Recently Doench has been working on developing CRISPR pooled libraries for use in ...  Resources at Addgene Find CRISPR resources Find CRISPR pooled libraries Learn about other members of...technology at the start of the CRISPR craze, but he says, “In the case of CRISPR, the hype was completely justified...biological process X, you can do with CRISPR technology...” and “with CRISPR, just the results are so much cleaner...understanding, curation, and explanation of our CRISPR resources. We recently sat down with Dr. Doench...and has contributed much of his knowledge to the CRISPR resources at Addgene. His background in history... technology.” Doench thinks that the future of CRISPR will be in high throughput functional characterization...

  11. CRISPR Cheat Sheet

    Type
    Blog Post
    Published
    Jan. 27, 2021, 2 p.m.
    ...Applications of CRISPR: Cut: CRISPR can be used to cut the DNA sequence. With a repair template, CRISPR can be...Jennifer Tsang. We have some crisp new CRISPR material for you!  The CRISPR field moves fast. That’s why we’...Addgene’s CRISPR 101 eBook. This new edition of the eBook includes the details on advances in CRISPR including... Prime editing Anti-CRISPRs … you name it! So get excited and download the CRISPR eBook today! P.S - ...refresher before you dive into the CRISPR eBook, check out the CRISPR Cheat Sheet. Download the PDF using...same information. Download a PDF of CRISPR Cheat Sheet here!   CRISPR Cheat Sheet Clustered Regularly Interspaced...applied goal. Components of CRISPR: gRNAs: Sequences of RNA that direct the CRISPR system to cut other DNA...

  12. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...this method. Step 1: Prepare a random fragment library of phage DNA This first step involves digesting...  Ligate and transform your resultant plasmid libraries into chemically competent E. coli and analyze ...for appropriately sized inserts. We consider a library worth using if at least 40% of colonies tested ... liquid culture, dilute and plate the complete library of transformants for single colonies (this usually...competent strain that you can use for long-term library storage. After overnight growth, scrape up, pool...well, the proteins encoded by the plasmids in the library will remain attached to the membrane at the same... or to any “sticky” proteins expressed by your library. After blocking for 1 h at room temperature with...

  13. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...       The CRISPR corner By Jennifer Tsang Here are a few highlights from recent CRISPR plasmids. To...To find all of the CRISPR plasmids available from Addgene, head over to our CRISPR Plasmids and Resources... very fast CRISPR, is a light-inducible CRISPR system developed by Taekjip Ha’s lab where Cas9-gRNA complex...different antibiotic resistance markers. New CRISPR pooled library from Keith Joung’s and Benjamin Kleinstiver...auxin inducible degron system for C. elegans New CRISPR plasmids New items from the viral service Epigenetic...Saptrap cloning systems to generate AID* tagged CRISPR knock-in repair constructs. These repair constructs...tagged AID* into genomic sites of interests via CRISPR techniques. This tool-kit includes an array of ...

  14. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...PMC4273606. 4. Improved vectors and genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F...Plasmid FAQs Answered Learn How Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening Read...Lentiviral vectors are the vector of choice for many CRISPR applications, and they’ve also had success in clinical...difficult-to-transfect cells. Lentiviral barcoding libraries have been used for complex applications like lineage...transgene-expressing or knockout cell lines. Many CRISPR plasmids are designed for lentiviral use, including...Read about Genome-Wide Screening Using CRISPR/Cas9 Additional Resources on Addgene.org Check out Our...including Addgene’s top requested plasmid in 2015: lentiCRISPR v2, used both for targeted modification and genome-wide...

  15. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Jan. 23, 2024, 2:15 p.m.
    ...the human transcriptome. The MORF library is only one of 22 libraries, created from 14 deposits, that became...countries around the world. That included 2008 pooled libraries, 290 antibodies, 18,359 viral items, and 166,247...collection, a comprehensive transcription factor library, in lentiviral vectors. At 3500 plasmids, this ...the recent release of the Spanish version of our CRISPR 101 eBook and the upcoming release of the second...

  16. Introducing an All-in-One CRISPR/Cas9 Vector System for Multiplex Genome Engineering

    Type
    Blog Post
    Published
    Nov. 12, 2014, 3:54 p.m.
    ...gRNA for CRISPR Genome Editing CRISPR-Cas9: Tips for Optimizing sgRNA Activity Lentiviral CRISPR Libraries...tandem. They went on to show that their CRISPR/Cas9-nuclease and CRISPR/Cas9-nickase vectors work successfully...Find the Mulitplex CRISPR/Cas9 Assembly System Kit at Addgene here. Other Popular CRISPR Posts: How to ...established all-in-one vector construction system for CRISPR/Cas9-mediated multiplex genome engineering is now...multiplexity is one of the most advantageous properties of CRISPR/Cas9 compared to ZFNs and TALENs,” said Tetsushi...system offers ease and efficiency for all-in-one CRISPR/Cas9 vectors expressing up to seven guide RNAs ... Figure 1: Schematic overview of the all-in-one CRISPR/Cas9 vector construction system for multiplex genome...

  17. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...information on these new CRISPR screening tools, see our CRISPR/Cas Plasmids: Pooled Libraries webpage or read ...Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening. Also, check out our updated CRISPR-Cas ...Microbiology 2011 Feb 22;11:38. Lentiviral CRISPR libraries for knockout screening New systems have been...knockout screens. These vectors and sgRNA libraries expand upon the CRISPR family of plasmids by offering a lentivirus-based...CRISPR-Cas resources at www.addgene.org/CRISPR/ to browse CRISPR plasmids, watch informational videos, download...for background information on CRISPR technology? See our improved CRISPR-Cas Guide. Wang et al., Science...Tet-inducible CRISPR activators and repressor plasmids. (Kearns et al., Development. 2014..) CRISPRs for Xenopus...

  18. 8 Great Depositor Research Stories

    Type
    Blog Post
    Published
    Dec. 15, 2022, 2:15 p.m.
    ...their new CRISPR tools, Cas7-11, a low-toxicity RNA editing tool, and PASTE, a drag-and-drop CRISPR editing...their paper uncovering ancestral bias in CRISPR guide library design.  dCas9-directed DNA Demethylation...minimal payloads!) Uncovering Ancestral Bias in CRISPR Research: We spoke to researchers from the Boehm... how to use dCas9 (with no modifications!) as a CRISPR/dCas9-based tool for DNA demethylation. AAV Q&A...

  19. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type
    Blog Post
    Published
    June 6, 2019, 12:41 p.m.
    ...using the CRISPR-Cas9 system CHIME Article contributed by Aliyah Weinstein  Listen to the CRISPR podcast...human miRNAs. Weiss’s collection consists of a library of reporter plasmids, each containing two fluorescent...require custom nucleases for each gene of interest. CRISPR-Cas9 tools have been used in immunology for in ...vitro screens of immune cell activity. However, a CRISPR-based tool for in vivo functional analysis of immune...Harvard Medical School developed a solution: CHIME, a CRISPR-Cas9 system that can edit genes in any immune lineage...used for high throughput screens using pooled libraries. Find the CHIME plasmids at Addgene. LaFleur et...

  20. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...lentiviral inventory is based on CRISPR tools, though we do provide a few non-CRISPR related viral preps. Cas9...be used both for screens (using pooled CRISPR knockout libraries) and individual gene knockouts (using ...Zhang F. Improved vectors and genome-wide libraries for CRISPR screening. Nat Methods. 2014 Aug;11(8):783...Cas9 is the headliner of most CRISPR applications and can be expressed from your cell line of choice after...
Showing: 41 - 60 of 109 results