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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 57 results for: dcas9, grna

Showing: 41 - 57 of 57 results

  1. Editor's Choice, August 2016

    Type
    Blog Post
    Published
    Sept. 2, 2016, 1:32 p.m.
    ...endonuclease (dCas9) fused to a deaminase to make the transitions at target sequences specified by gRNAs. Caveats...

  2. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...CRISPRi uses a nuclease-dead Cas9 protein (dCas9) and a sgRNA to temporarily block the transcription machinery...loss (Ho et al. 2014). To overcome this, paired gRNAs can be used to target specific sizes of a DNA fragment..., removing large lncRNA fragments with multiple gRNAs poses the risk of disrupting adjacent genes, especially...Cas12a variant can cleave single-stranded RNA using gRNAs to specifically edit or degrade targeted lncRNAs...around 20 base pairs long. Using a single guide RNA (sgRNA) to create a double-strand break works well because...locus (Ho et al. 2012). For large-scale screening, sgRNA libraries are useful for targeting thousands of ...

  3. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...scarless insertion of the payload (teal). The Cas9 gRNA targeting guide and the transposon RNA homology ...TurboCas provides the genomic targeting power of dCas9 with the dynamic labeling power of miniTurboID, ...proximity ligation tool (Cenik et al., 2024). While dCas9 has been used in combination with proximity labeling...background labeling. By fusing miniTurboID with dCas9 to create TurboCas, the Shilatifard Lab created ...

  4. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...the gene into a gRNA finder such as WU-CRISPR (Wong et al., 2015)) or our lab’s sgRNA scorer 1.0 (Now ...environmental triggers. This is done by creating a split dCas9 protein that can reassemble when exposed to specific...uses for Cas9 activators is in genetic screening. gRNAs targeting every gene in the human genome for example...targeted by the activator by simply providing a new gRNA rather than engineering an entirely new protein....difficult to construct and deliver when compared to gRNAs. Additionaly, cDNAs cannot be used to study in cis...

  5. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...multiplexing; expressing 5 gRNAs simultaneously produces comparable gene knockdown to single gRNA expression. Although... target RNA without cleaving the molecule (like dCas9) suggests that dCas13a may be useful for isolating....org Browse All CRISPR Plasmids Find Validated gRNAs for Your Next Experiment Check Out Our CRISPR Guide...

  6. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...nickase) fused to a deaminase that makes the edit A gRNA targeting Cas to a specific locus A target base ...coupling a cytidine deaminase with the inactive dCas9 (Komor et al., 2016). These fusions convert cytosine...an inhibitor of uracil DNA glycosylase (UGI) to dCas9 prevents base excision repair, which would change.... To do so, they used a Cas nickase, instead of dCas9. The resulting editor, BE3, nicks the unmodified...CRISPR plasmids for base editing Find validated gRNAs ...

  9. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type
    Blog Post
    Published
    June 27, 2018, 1:16 p.m.
    ...genomic loci. He made use of catalytically inactive dCas9 by fusing it to an engineered peroxidase, (APEX)... used this technology, which they call C-BERST (dCas9–APEX2 biotinylation at genomic elements by restricted...This ‘Caspex’ construct is guided by a pre-defined sgRNA sequence to a specific genomic locus and labels ... and targeted to a sequence of interest with an sgRNA expressed from a separate plasmid. When cells are...

  10. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...coated beads. Fluorescently labelled dCas9 complexes containing sgRNA that have no mismatches bind to the... Cas13, upon finding complementarity between the gRNA and a given fluorescently labelled substrate, gets... E and the N2 gene of SARS-CoV-2. If the Cas12a-sgRNA complex binds to the target sequences, Cas12a activity...

  11. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...tRNA adenosine deaminases (ecTadA), fused it with dCas9, and transformed it into E. coli harboring the defective...expression system. We use a single polycistronic tRNA-gRNA (PTG) gene system to clone multiple protospacers...

  12. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ... Acronym Full name Notes CRISPR terms gRNA/sgRNA Guide RNA RNA used by Cas9 to locate a matching...Base editors use either catalytically dead Cas9 (dCas9) or a Cas9 nickase (nCas9) to avoid introducing ... the DNA strand that does not base pair with the gRNA. Base editors target this “non-target strand.” To... predicting CGBE editing outcomes and assisting sgRNA selection is available at http://www.sunlab.fun:...

  13. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...1143-8.  A new tool for CRISPR gRNA validation Selecting a gRNA sequence that effectively targets...DNA break. Next, the gRNA being tested is expressed along with Cas9. If the gRNA effectively cuts the ...for scientists to validate the efficacy of their gRNAs. The first step is to clone the target sequence ...CRISPRS. Developed by David Liu and colleagues, FokI-dCas9 expresses Fok1 nuclease domain fused to catalytically...genome-wide knockout screens. These vectors and sgRNA libraries expand upon the CRISPR family of plasmids...plasmids by offering a lentivirus-based mechanism for sgRNA delivery and providing a means for large scale functional...

  15. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...of applications, including CRISPR activation by dCas9-VPR, prime editing, and delivery of disease-relevant...engineered pegRNAs (epegRNAs), which add a structural motif to stabilize the 3’ end of the pegRNA. As research...The team designed sgRNAs that include an internal barcode and separated multiple sgRNAs with tRNAs, which...BFP2.     Figure 1: CROPseq-multi uses two sgRNAs with internal barcodes (iBARs), multiplexed using...lentiviral integration, producing a second copy of the sgRNAs. Image reused from Walton et al. 2024 under a CC-BY-NC-ND...occurs on the RNA polymerase III transcript, the sgRNAs were encoded on the lentiviral minus strand in ...of host cell proteins, and modifications to the pegRNA. The Britt Adamson Lab recently used a CRISPRi ...

  16. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ...lab. The Bikard library contains ~93,000 unique gRNAs that target random positions along the E. coli MG1655...targeting requires two sgRNAS: one that targets the knock-in site and a universal sgRNA that targets the donor... the genomic loci of interest. As the universal sgRNA is not predicted to target sites in the human, pig.... The lab has previously used the library with SpdCas9 to perform pooled CRISPRi screens to identify essential...

  17. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ... the Zhang lab expressing S. pyogenes Cas9 and a gRNA cassette. It can be used to produce a ~10-fold higher...and selection marker removal. CRISPR technology pdCas9-humanized (Plasmid #44246) Expression of a catalytically...
Showing: 41 - 57 of 57 results